Additional file 2: Figure S2. of Relaxin deficiency results in increased expression of angiogenesis- and remodelling-related genes in the uterus of early pregnant mice but does not affect endometrial angiogenesis prior to implantation

Expression of (a) 18 s, (b) PpiA, (c) Sdha and (d) Tbp in the uterus of Rln +/+ mice on days 1 to 4 of pregnancy (n = 6–8). Horizontal bars indicate mean values. Groups that do not share a letter are significantly different from one another (p < 0.05). (PDF 37 kb

Additional file 3: Table S1. of Relaxin deficiency results in increased expression of angiogenesis- and remodelling-related genes in the uterus of early pregnant mice but does not affect endometrial angiogenesis prior to implantation

Primer and probe sequences for the quantitative amplification of murine genes. Table S2. Mean CT values and the range of CT values for each gene analyzed by qPCR in the uterus of wildtype (Rln +/+ ) and relaxin deficient (Rln -/- ) mice (n = 6–8). Table S3. Primer sequences for the RT-PCR amplification of murine genes. (DOC 111 kb

Charged residues on the side of the nucleosome contribute to normal Spt16-gene interactions in budding yeast

<p>Previous work in <i>Saccharomyces cerevisiae</i> identified three residues located in close proximity to each other on the side of the nucleosome whose integrity is required for proper association of the Spt16 component of the FACT complex across transcribed genes. In an effort to gain further insights into the parameters that control Spt16 interactions with genes <i>in vivo</i>, we tested the effects of additional histone mutants on Spt16 occupancy across two constitutively transcribed genes. These studies revealed that mutations in several charged residues in the vicinity of the three residues originally identified as important for Spt16-gene interactions also significantly perturb normal association of Spt16 across genes. Based on these and our previous findings, we propose that the charge landscape across the region encompassed by these residues, which we refer to as the Influences Spt16-Gene Interactions or ISGI region, is an important contributor to proper Spt16-gene interactions <i>in vivo.</i></p