Detection of polyomaviruses in gastrointestinal and lymphoid organs with generic PCR.

a<p>Detected with generic PCR.</p

Phylogenetic analysis of HPyV12.

<p>A Bayesian chronogram was deduced from the analysis of a 488 amino acid alignment of LTAg sequences. Polyomaviruses identified from human hosts are in red, from apes in blue. The human polyomaviruses MXPyV and the US strain of MWPyV have the same phylogenetic position as HPyV10 and are not shown. The sequence of the very recently discovered human STLPyV which is most closely related to HPyV10, MWPyV and MXPyV, became only available at the end of the revision process of this manuscript; it was therefore not included in the datasets put together for the present study. Statistical support for branches is given as posterior probability/bootstrap. For three branches defining potentially meaningful bipartitions (with respect to the question of the phylogenetic placement of HPyV12), statistical support observed for the corresponding bipartitions in the VP2 and VP1 analyses is also shown (grey panels). Hyphen indicates that bipartition was not observed in the ML or Bayesian tree. The scale axis is in amino acid substitution per site. This chronogram was rooted using a relaxed clock. A maximum likelihood analysis of the same dataset concluded to a similar topology and is thus not shown here.</p

Detection of HPyV12 with PCR.

a<p>Samples collected for BKPyV infection diagnosis.</p>b<p>From patients under natalizumab (Tysabri) therapy.</p>c<p>Samples collected for herpesvirus infection diagnosis.</p>d<p>Samples collected for JCPyV infection diagnosis.</p>e<p>Combined results of specific nested PCR and real-time PCR; samples were considered as positive if the result could be independently reproduced on different days.</p

Genome organization of HPyV12.

<p>Putative coding regions for VP1 to VP3, STAg antigen, and LTAg antigen are marked by arrows.</p

Reactivity of human sera to VP1 of HPyV12.

<p>The percentage of seroreactivity of pediatric sera (n = 74; 2–11 years) and sera of healthy adolescents and adults (n = 299; age: 16–72 years) in ELISA is shown. The results were stratified by age.</p