2 research outputs found
Optical Voltage Sensing Using DNA Origami
No full textWe explore the potential
of DNA nanotechnology for developing novel
optical voltage sensing nanodevices that convert a local change of
electric potential into optical signals. As a proof-of-concept of
the sensing mechanism, we assembled voltage responsive DNA origami
structures labeled with a single pair of FRET dyes. The DNA structures
were reversibly immobilized on a nanocapillary tip and underwent controlled
structural changes upon application of an electric field. The applied
field was monitored through a change in FRET efficiency. By exchanging
the position of a single dye, we could tune the voltage sensitivity
of our DNA origami structure, demonstrating the flexibility and versatility
of our approach. The experimental studies were complemented by coarse-grained
simulations that characterized voltage-dependent elastic deformation
of the DNA nanostructures and the associated change in the distance
between the FRET pair. Our work opens a novel pathway for determining
the mechanical properties of DNA origami structures and highlights
potential applications of dynamic DNA nanostructures as voltage sensors
Optical Voltage Sensing Using DNA Origami
No full textWe explore the potential
of DNA nanotechnology for developing novel
optical voltage sensing nanodevices that convert a local change of
electric potential into optical signals. As a proof-of-concept of
the sensing mechanism, we assembled voltage responsive DNA origami
structures labeled with a single pair of FRET dyes. The DNA structures
were reversibly immobilized on a nanocapillary tip and underwent controlled
structural changes upon application of an electric field. The applied
field was monitored through a change in FRET efficiency. By exchanging
the position of a single dye, we could tune the voltage sensitivity
of our DNA origami structure, demonstrating the flexibility and versatility
of our approach. The experimental studies were complemented by coarse-grained
simulations that characterized voltage-dependent elastic deformation
of the DNA nanostructures and the associated change in the distance
between the FRET pair. Our work opens a novel pathway for determining
the mechanical properties of DNA origami structures and highlights
potential applications of dynamic DNA nanostructures as voltage sensors
