Investigating the association between intestinal parasite infection and cribra orbitalia in the medieval population of Cambridge, UK

ObjectiveCribra orbitalia is believed to be a skeletal indicator of chronic anaemia, scurvy, rickets or related metabolic diseases. It has been suggested that it may be used as a proxy indicator for intestinal parasite infection, as parasites often cause anaemia today. Our aim is to investigate this association in the medieval population of Cambridge, UK.MaterialsIndividuals excavated from the cemeteries of the Augustinian friary and All Saints by the Castle parish church, and aged from 7 to adulthood.MethodsWe undertook parasite analysis of the pelvic sediment and control samples of 46 burials with intact orbital roofs.ResultsHuman roundworm (Ascaris lumbricoides) and/or whipworm (Trichuris trichiura) were identified in the pelvic sediment of 22 individuals, and cribra orbitalia noted in 11 individuals. Barnards test showed no association between parasite infection and cribra orbitalia (p = .882).ConclusionWe found no association between infection and cribra orbitalia infection in this medieval adult population, calling into question this hypothesis, at least for adults.SignificanceHigh or low cribra orbitalia prevalence in adults should not be used to infer rates of intestinal parasite infection.LimitationsThe individuals in the study were over the age of 7, with no younger children. It is possible that only parasites which cause marked anaemia (such as hookworm, schistosomiasis or malaria) may cause cribra orbitalia, while less marked anaemia from roundworm and whipworm may not do so.Suggestions for further researchRepeating this study in younger children, when most cribra orbitalia appears to form

Test of the lateral angle method of sex estimation on Anglo‐Saxon and Medieval archaeological populations with genetically estimated sex

The lateral angle method of sex estimation is tested on an archaeological population with genetic sex estimates. Casts of the internal auditory canal were made using a quick drying impression material on 90 individuals (76 adults and 14 nonadults) from Anglo-Saxon and Medieval Cambridgeshire. The anterior and posterior angles of the internal auditory canal were measured, and the relationship of the angle to genetic sex was tested. The posterior angle failed intra-observer error tests, and only the anterior angle could be analysed. Using the previously published sectioning point for unburnt remains (45°), the method did not adequately distinguish between the sexes. Furthermore, the difference between male and female was insufficient to create population-specific discriminant functions. The anterior angle does not meet the requirements for an osteological method of sex estimation, exhibiting no statistical correlation with genetic sex in this population.</p

Ancient Mycobacterium leprae genome reveals medieval English red squirrels as animal leprosy host

Leprosy, one of the oldest recorded diseases in human history, remains prevalent in Asia, Africa, and South America, with over 200,000 cases every year.1,2 Although ancient DNA (aDNA) approaches on the major causative agent, Mycobacterium leprae, have elucidated the disease’s evolutionary history,3,4,5 the role of animal hosts and interspecies transmission in the past remains unexplored. Research has uncovered relationships between medieval strains isolated from archaeological human remains and modern animal hosts such as the red squirrel in England.6,7 However, the time frame, distribution, and direction of transmissions remains unknown. Here, we studied 25 human and 12 squirrel samples from two archaeological sites in Winchester, a medieval English city well known for its leprosarium and connections to the fur trade. We reconstructed four medieval M. leprae genomes, including one from a red squirrel, at a 2.2-fold average coverage. Our analysis revealed a phylogenetic placement of all strains on branch 3 as well as a close relationship between the squirrel strain and one newly reconstructed medieval human strain. In particular, the medieval squirrel strain is more closely related to some medieval human strains from Winchester than to modern red squirrel strains from England, indicating a yet-undetected circulation of M. leprae in non-human hosts in the Middle Ages. Our study represents the first One Health approach for M. leprae in archaeology, which is centered around a medieval animal host strain, and highlights the future capability of such approaches to understand the disease’s zoonotic past and current potential.</p