<i>Mycobacterium avium</i> subspecies <i>paratuberculosis</i> bacteremia in type 1 diabetes mellitus: an infectious trigger?

Mycobacterium avium subspecies paratuberculosis (MAP) is the established cause of paratuberculosis in ruminants (i.e., Johne disease). The bacterium is shed in the milk of infected cows and survives pasteurization. Recently, an association between MAP and Crohn disease has been suggested, wherein MAP has been found to persist in a cell wall–deficient form, escaping clearance by the host immune system

Sperimentazione di un nuovo training per cani co-terapeuti e per supporto disabili secondo un approccio neuropsicologico

The aim of this work was to evaluate the neuro-psychological method based on the Cognitive Behavioural Method modified, in the training of assistance and co-therapist dogs working in Animal-Assisted Interventions (AAI). This approach is based on the relationship between dog and handler and dog’s training is oriented to increase its experiences, its adaptability and its cognitive skills whereas classic dog handling forecasts creation and management of automatisms, with the dog kept always under control. Twenty-tree dogs of different breeds were involved in this study. The esteemed time to train a dog for AAI is resulted to be 1 year but the complete behavioural development is reached around the 2 years. The dogs learned almost 50 words, they were able to answer simple and complex requests, to generalize starting from a noun-object association and/or from a given action. During both everyday’s life and AAI, they showed a spontaneous collaborative attitude. A relevant result was that the dogs participated to 1 to 15 AAI session a week, lasting 30 minutes each, never showing any behavioural sign related to stress. An important feature of the training was the relationship between dog and handler, built through correct communication and attention to the cognitive characteristics of the dogs, as the constitution of the dog-handler couple is one of the prerequisites to participate to AAI. This method permits to train dogs respecting their ethological and behavioural characteristics

Flood damage risk assessment optimizing a flood mitigation system

Flood risk management plan for potentially damaged areas is a main requirement from the European Directive 2007/60. Therefore, assessment and evaluation of the potential flood damages in quantitative terms need to be defined in mapped flood risk areas. Quantitative evaluation should be in agreement with the required cost-benefit analysis as a rational decision-making tool optimizing the flood mitigation system. In the first part of the paper, analyses are reported, based on recent flood events that had taken place in the Europe, in order to obtain depth-damage functions to apply in the Sardinia region (Italy). Flood damage functions are not still available for every European country and the paper analysed previous studies to define depth-damage functions for this region. The paper focuses then on the validation of the proposed depth-damage functions. The available flood data and reported damages of the massive storm happened in South-Est Sardinia the 22nd of October 2008 are analysed. Data analysis allows to compute the total economic losses for different land uses conditions and to compare with the evaluated ones. In the last part of the paper, expected damages evaluated using depth-damages functions are used for the Coghinas river lowlands valley. The regional Water District Authority defining the flood risk management plan considers this river basin as a pilot area. Applying hydraulic models to floodplain river flow allows evaluation of the expected flood damages using the depth-damage functions. Moreover, the procedure allows developing the cost-benefit analysis in order to justify different design scenarios and gives a rational decision-making tool optimizing the flood mitigation syste

Social Withdrawal and Mental Health: An Interdisciplinary Approach

Social isolation may be considered as a risk factor for health. It may contribute to the development of a mental health disease. In this chapter, social withdrawal is defined as voluntary isolation prolonged in time that involves the cessation of any form of social relationship and contact with people and the outside. Clinical psychology, psycho-educational interventions, and social neuroscience research tries to understand what happens when social isolation is experienced. Therefore an interdisciplinary perspective can help to better understand this phenomenon. The deepening of these aspects can help to create new forms of theoretical perspective and of a clinical and psycho-educational intervention to better arrange for this new type of maladaptive condition

Genetic diversity of Sardinian goat population based on microsatellites

During the last century, the selection for production traits of the main livestock species has led to a reduction in number of local populations with consequent loss of genetic variability. In Sardinia, the genetic improvement strategy has been based on selection for the local pure breed in sheep, whereas in the other species (cattle, swine and goat), an often unplanned crossbreeding with improved breeds has been applied. In this context, several studies on genetic diversity of these populations have been started with the final aim of recovering the autochthonous genetic types. The global approach involves different steps: - characterization of the farming system and typical products; - morphological and productive evaluation of the animals; - genetic characterization of the populations through molecular DNA analyses (microsatellites, AFLP, mitochondrial DNA, etc.); - creation of nucleus flocks in public farms and germoplasm banks; - organization of herd-books involving farmers interested in the safeguard of local breeds and valorisation of traditional products. A far as the goat population is concerned, Sardinia is the Italian region with the largest goat stock, (209.000 heads, about 23% of the total national stock). Farms are located in mountains, low hills and even plans (Macciotta et al., 2002) resulting in a quite large variability (Brandano and Piras, 1978) in terms of feeding and management techniques; but extensive and semi extensive systems in marginal areas prevail (Carta et al., 2001; Usai et al., 2004). The current population has been constituted by crossbreeding the autochthonous animals with other improved Mediterranean breeds,mainly Maltese goat. The aim of this paper is to study the genetic variability of this population through the analysis of 17 microsatellite markers

Interaction between <i>Mycobacterium tuberculosis</i>, <i>Mycobacterium bovis</i>, <i>Mycobacterium avium</i> subspecies <i>paratuberculosis</i> with the enteric glia and microglial cells

Background We investigated the interaction of Mycobacterium avium subspecies paratuberculosis, M. bovis and M. tuberculosis and different glial cells (enteric glial and microglial cells) in order to evaluate the infecting ability of these microorganisms and the effects produced on these cells, such as the evaluation of cytokines expression. Results Our experiments demonstrated the adhesion of M. paratuberculosis to the enteroglial cells and the induction of IL-1A and IL-6 expression; M. tuberculosis and M. bovis showed a good adhesive capability to the enteric cell line with the expression of the following cytokines: IL-1A and IL-1B, TNF-α, G-CSF and GM-CSF; M. bovis induced the expression of IL-6 too. The experiment performed with the microglial cells confirmed the results obtained with the enteroglial cells after the infection with M. tuberculosis and M. bovis, whereas M. paratuberculosis stimulated the production of IL-1A and IL-1B. Conclusion Enteroglial and microglial cells, could be the target of pathogenic mycobacteria and, even if present in different locations (Enteric Nervous System and Central Nervous System), show to have similar mechanism of immunomodulation

Prime valutazioni degli effetti della protezione sull’ittiofauna di fondo roccioso nella costa occidentale dell’Isola dell’Asinara = Preliminary evaluation of the effects of protection on rocky fish assemblages in the Western coast of Asinara Island

Coastal fish fauna from a recently established Mediterranean MPA and from an adjacent nonprotected zone near its boundary were compared to evaluate the effects of protection. A remarkable “reserve effect” was detected for the whole assemblage in terms of both species richness and abundance. Moreover, a moderate spillover effect outside the MPA was observed for several target species

Estimation of (co)variance components of nematode parasites resistance and somatic cell count in dairy sheep

Nematode parasites and mastitis are the major animal health constraints in sheep. The aim of this study was estimating the genetic (co)variances of nematode parasites resistance and somatic cell count in dairy sheep. From 2000 to 2008, Somatic Cell Score (SCS) and Faecal Egg Count (FEC) records were available on an experimental population consisting of 949 backcross ewes and 806 their daughters. Data were processed independently for each subpopulation in order to adjust for specific environmental effects and to obtain lactation records for both traits to be used in the genetic analysis. Variance components estimation was performed by using the REML method applied to a bi-trait repeatability animal model. Heritabilities of lactation SCS (LSCS) and FEC were 0.19 and 0.16. Genetic correlation was 0.21, whereas phenotypic correlation was 0.01. The estimated heritabilities confirm that both traits could be selected by the classical quantitative approach. The genetic correlation estimate between LSCS and FEC suggests that selection for one of the two traits would not have any detrimental effect on the other one

"In vitro" activities of antimycobacterial agents against <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> linked to Crohn's disease and paratuberculosis

Crohn's disease, a human disease similar to paratuberculosis in animals is the most painful and devastating disease that may involve infection with M. avium subsp. paratuberculosis (MAP), different genetic polymorphisms and an immune dysregulation syndrome. Treatment of Crohn's disease is most commonly based on 5-aminosalicylic acid (5-ASA) compounds, corticosteroids, and immunosuppressive agents. Recently, biological therapies using monoclonal antibodies against inflammatory cytokines have shown some positive results. However, all these therapies treat the symptoms not the cause of the disease