Selective Cytotoxic Activity of Anredera Cordifolia Leaves Extract Towards Hela Cells

Cervical cancer is second rank cancer in female cancer incidence all over the world and the strategy therapy against this disease is addressing cancer cells without endanger normal cells. Discovering potentially selective anticancer agent from plants for the treatment of cervical cancer has become a very challenging area of research worldwide. Our previous study Anredera cordifolia, commonly named as binahong in Indonesia, revealed cytotoxic activity on HeLa cervical cancer cells with IC50 75 µg/mL. However, the selectivity of the chemical agent and its molecular target has still remained a question. The current study was aimed at investigating the selectivity of ethanolic extract of A. cordifolia leaf (EAC) on Vero cells and its molecular target on HeLa cells. The extracts were prepared by macerating Anredera cordifolia leaf powder in 70% ethanol. The assessment of the viability of Vero cells was carried out using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; while the cell cycle analysis of HeLa cells was probed by using flow cytometry. Based on the cell cycle analysis, the molecular target of the extract was investigated by immunocytochemical staining. The present study exhibited the selective cytotoxicity of EAC on HeLa cells compared to Vero cells with a Selectivity Index (SI) of 17.36. It arrested cell cycle on G1/S phase and suppressed Bcl–2 expression, anti-apoptotic protein, which also regulates cell cycle. Therefore, the current piece of work endorses the use of EAC as a promising anticancer agent in the treatment of cervical cancer. EAC may be used as a selective anticancer agent on HeLa cells

Desain Dan Implementasi Sistem Kendali CNC Router Menggunakan PC Untuk Flame Cutting Machine

This paper focuses on design of router control systems based on computer numerical control (CNC) using personal computer (PC) implemented in flame cutting machine (FCM). NC-Code entered into the computer translated to be a command signal sent by the PC to a microcontroller to control the end effector's movement alongthe X and Y axis simultaneously based on linear and circular interpolations calculation on the PC. This control system is implemented on FCM by connecting the output control of the microcontroller with the driver actuator of the FCM in the form of a DC motor. The obtained result is in the form of a CNC router control system prototype to be implemented in the FCM which is capable to perform linear interpolation and circular interpolation