Additional file 1: Table S1. of An ELISA assay using a combination of recombinant proteins from multiple strains of Orientia tsutsugamushi offers an accurate diagnosis for scrub typhus

Correlation of IFA titer and ELISA in acute samples. Table S2. Distribution of IgG or IgM ELISA positive but diagnosed as scrub typhus negative. Figure S1. Correlation of IgG and IgM ELISA OD with single IFA titers at 400. One of the criteria to consider a patient as ST positive was based on IgG or IgM titer > = 400 of a single serum. The ELISA OD of patients was plotted against the IgG or IgM titer <400 (negative, open symbols) or > = 400 (positive, closed symbols). The mean OD of each group was plotted in red. The mean and standard deviation of ELISA OD of IgG (circles) or IgM (triangles). IFA positives (IFA titer > = 400) was significantly different from that of IFA negatives (t test, p < 0.0001). Figure S2. Correlation of IgG and IgM ELISA OD with ST cases. ST cases were determined by a combination of IFA and PCR results. The ELISA OD of patients who were determined as positives (closed symbols) and negatives (open symbols) were plotted. The mean and standard deviation of ELISA OD of each group was plotted in red. The mean OD of IgG (circles) or IgM (triangles) for determined positives and negatives was significantly different (t test, p < 0.0001). (DOCX 14 kb

Costs, clinical outcomes and antibiotic prescription at various prevalence rates and for patients receiving no treatment, empirical treatment, diagnosis using the latex test and diagnosis with a test with 95% sensitivity and specificity.

<p>Costs, clinical outcomes and antibiotic prescription at various prevalence rates and for patients receiving no treatment, empirical treatment, diagnosis using the latex test and diagnosis with a test with 95% sensitivity and specificity.</p

Test performance using acute sera [10],[14].

<p>Test performance using acute sera <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0000610#pntd.0000610-Kemapunmanus1" target="_blank">[10]</a>,<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0000610#pntd.0000610-Arimitsu1" target="_blank">[14]</a>.</p

Values and sources of inputs used in the decision model.

<p>Values and sources of inputs used in the decision model.</p

Costs (USD).

a<p>Cost of doxycycline (100 mg) 14 capsules in Thailand</p>b<p>Cost as of 2004 of lateral flow, MCAT, latex test.</p>c<p>Treatment of side effect symptoms such as antiemetic drug or antihistamine for rash.</p><p>With work loss, 1 day at minimum earning (5.25 USD) assumed.</p><p>There is no additional cost to patients not receiving antibiotics or to being cured.</p>d<p>Estimate of hospital costs, including intravenous antibiotics, other treatment.</p

Benefit-cost ratios for the different strategies.

*<p>The negative value is a result of the empirical treatment strategy being both less expensive and more effective than the <i>no –antibiotic</i>- treatment baseline</p