Amyloid precursor protein 770 is specifically expressed and released from platelets

博士（医学）福島県立医科大

Epigenetic Regulation of Intronic Transgenes in Arabidopsis

Defense mechanisms of plant genomes can epigenetically inactivate repetitive sequences and exogenous transgenes. Loss of mutant phenotypes in intronic T-DNA insertion lines by interaction with another T-DNA locus, termed T-DNA suppression, has been observed in Arabidopsis thaliana, although the molecular basis of establishment and maintenance of T-DNA suppression is poorly understood. Here we show that maintenance of T-DNA suppression requires heterochromatinisation of T-DNA sequences and the nuclear proteins, INCREASED IN BONSAI METHYLATION 2 (IBM2) and ENHANCED DOWNY MILDEW 2 (EDM2), which prevent ectopic 3′ end processing of mRNA in atypically long introns containing T-DNA sequences. Initiation of T-DNA suppression is mediated by the canonical RdDM pathway after hybridisation of two T-DNA strains, accompanied by DNA hypermethylation of T-DNA sequences in the F1 generation. Our results reveal the presence of a genome surveillance mechanism through genome hybridisation that masks repetitive DNAs intruding into transcription units

El marketing relacional y la generación de valor en el cliente de la empresa Viettel Perú S.A.C., Lima Metropolitana 2020

Esta investigación tuvo como objetivo general analizar de qué manera el marketing relacional contribuye en la generación de valor de los clientes de la empresa Viettel Perú S.A.C., Lima Metropolitana 2020. Este estudio fue de enfoque cuantitativo, tipo básica, nivel correlacional y de diseño no experimental de corte transversal. La población la conformaron 389045 personas que son la totalidad de la población comprendida por los distritos Jesús María, Pueblo Libre, Magdalena del Mar, San Miguel y Lince, según datos del INEI; del cual se utilizó un muestreo estratificado con afijación proporcional, el cual con la muestra de 228 se obtuvo un número de encuestados determinado para cada distrito. La técnica que se empleó fue la encuesta, y el instrumento fue el cuestionario, del cual se obtuvo un estadístico global de fiabilidad de alfa de Cronbach de 0,970, lo que establece una fiabilidad muy alta, a su vez se obtuvo una validación de contenido por juicio de expertos de 98%, siendo una excelente calificación. Finalmente, se concluyó que, existe una relación entre el marketing relacional y la generación de valor del cliente, ya que se obtuvo coeficiente de Rho de Spearman de 0,378, correspondiente a una correlación positiva media. El marketing relacional ayuda a incrementar la generación de valor en la empresa Viettel Perú S.A.C

SAF-A promotes origin licensing and replication fork progression to ensure robust DNA replication

Funding CC was supported by a BBSRC EASTBIO Doctoral Training programme PhD studentship. SH was supported by Daiwa Anglo-Japanese Foundation 812 (12928/13746). Work in the Hiraga-Donaldson lab supported by Cancer Research UK awards C1445/A19059 and DRCPGM\100013. NG is supported by Medical Research Council (MC_UU_00007/13) Acknowledgements Information for SAF-A expression was obtained at The Cancer Genome Atlas TCGA) Research Network (https://www.cancer.gov/tcga). We thank Dr Ryu-suke Nozawa for help in the early stage of the project, and Professor Julian Blow for advice on the 3D licensing assay. Thanks to the staff of the Iain Fraser Cytometry Centre, and Microscopy and Histology facility at the University of Aberdeen.Peer reviewedPostprin

Absence of Tc-Pinning Phenomenon Under High Pressure in High-Entropy REO0.5F0.5BiS2 Layered Superconductor

Recently, robustness of superconductivity (transition temperature, Tc) under high pressures has been observed in high-entropy alloy (HEA), bcc-type Ti-Zr-Hf-Nb-Ta, and HEA-type compounds (Ag,In,Sn,Pb,Bi)Te with a NaCl-type structure. Since those materials have three-dimensional crystal structure, investigation on the pressure dependence of Tc of low-dimensional materials is needed to understand the phenomena. Here, we investigated the superconducting properties and the crystal structure of BiS2-based layered system REO0.5F0.5BiS2. Although the robustness of Tc was induced in MTe with increasing M-site configurational entropy, the increase in RE-site configurational entropy does not induce robustness of Tc under high pressures in REO0.5F0.5BiS2. The crystal structure of HEA-type REO0.5F0.5BiS2 was confirmed as monoclinic P21/m, which is the same space group as the zero-entropy counterpart LaO0.5F0.5BiS2. The results suggest that an increase in configurational entropy at blocking layers do not affect crystal structure and superconducting properties under high pressures in the BiS2-based layered system.Comment: 9 pages, 4 figure

Epigenetic regulation of spurious transcription initiation in Arabidopsis

In plants, epigenetic regulation is critical for silencing transposons and maintaining proper gene expression. However, its impact on the genome-wide transcription initiation landscape remains elusive. By conducting a genome-wide analysis of transcription start sites (TSSs) using cap analysis of gene expression (CAGE) sequencing, we show that thousands of TSSs are exclusively activated in various epigenetic mutants of Arabidopsis thaliana and referred to as cryptic TSSs. Many have not been identified in previous studies, of which up to 65% are contributed by transposons. They possess similar genetic features to regular TSSs and their activation is strongly associated with the ectopic recruitment of RNAPII machinery. The activation of cryptic TSSs significantly alters transcription of nearby TSSs, including those of genes important for development and stress responses. Our study, therefore, sheds light on the role of epigenetic regulation in maintaining proper gene functions in plants by suppressing transcription from cryptic TSSs

The chromosome-scale genome assembly of the yellowtail clownfish Amphiprion clarkii provides insights into the melanic pigmentation of anemonefish

Anemonefish are an emerging group of model organisms for studying genetic, ecological, evolutionary, and developmental traits of coral reef fish. The yellowtail clownfish Amphiprion clarkii possesses species-specific characteristics such as inter-species co-habitation, high intra-species color variation, no anemone specificity, and a broad geographic distribution, that can increase our understanding of anemonefish evolutionary history, behavioral strategies, fish-anemone symbiosis, and color pattern evolution. Despite its position as an emerging model species, the genome of A. clarkii is yet to be published. Using PacBio long-read sequencing and Hi-C chromatin capture technology, we generated a high-quality chromosome-scale genome assembly initially comprised of 1, 840 contigs with an N50 of 1, 203, 211 bp. These contigs were successfully anchored into 24 chromosomes of 843, 582, 782 bp and annotated with 25, 050 protein-coding genes encompassing 97.0% of conserved actinopterygian genes, making the quality and completeness of this genome the highest among all published anemonefish genomes to date. Transcriptomic analysis identified tissue-specific gene expression patterns, with the brain and optic lobe having the largest number of expressed genes. Further analyses revealed higher copy numbers of erbb3b (a gene involved in melanocyte development) in A. clarkii compared with other anemonefish, thus suggesting a possible link between erbb3b and the natural melanism polymorphism observed in A. clarkii. The publication of this high-quality genome, along with A. clarkii's many unique traits, position this species as an ideal model organism for addressing scientific questions across a range of disciplines

Endoplasmic Reticulum Associated Degradation of Spinocerebellar Ataxia-Related CD10 Cysteine Mutant

Spinocerebellar ataxia (SCA) is one of the most severe neurodegenerative diseases and is often associated with misfolded protein aggregates derived from the genetic mutation of related genes. Recently, mutations in CD10 such as C143Y have been identified as SCA type 43. CD10, also known as neprilysin or neuroendopeptidase, digests functional neuropeptides, such as amyloid beta, in the extracellular region. In this study, we explored the cellular behavior of CD10 C143Y to gain an insight into the functional relationship of the mutation and SCA pathology. We found that wild-type CD10 is expressed on the plasma membrane and exhibits endopeptidase activity in a cultured cell line. CD10 C143Y, however, forms a disulfide bond-mediated oligomer that does not appear by the wild-type CD10. Furthermore, the CD10 C143Y mutant was retained in the endoplasmic reticulum (ER) by the molecular chaperone BiP and was degraded through the ER-associated degradation (ERAD) process, in which representative ERAD factors including EDEM1, SEL1L, and Hrd1 participate in the degradation. Suppression of CD10 C143Y ERAD recovers intracellular transport but not enzymatic activity. Our results indicate that the C143Y mutation in CD10 negatively a ects protein maturation and results in ER retention and following ERAD. These findings provide beneficial insight into SCA type 43 pathology