GS and GA get activated in cerebral cortex and cerebellum of the ALF rats.

<p>Activity profiles of GS (A) and GA (B) from control and ALF rats. The values represent mean ± SD where n = 4 *p<0.05 (control vs ALF rats).</p

ALF did not induce alterations in the active level of GR in cerebral cortex and cerebellum.

<p>GR activity measured in cell free extract (A) and non-denaturing PAGE pattern of active GR (B and C). The technical details are same as described in Fig. 5.</p

TAA induces severe hepatic cell damage in the rats.

<p>(A) Light micrographs of hematoxylin - eosin stained liver sections from control (a and b) and TAA treated (c and d) rats wherein, a and c are photomicrographs at 10× and b and d at 40× magnifications. Arrow heads show centrilobular necrosis in the liver cells. Cells with small nuclear stains indicate infiltration of neutrophils and mononuclear lymphocytes. In B, pooled serum from 4–5 rats containing 10 µg protein in each lane was electrophoresed on 7.5% non-denaturing PAGE followed by substrate specific development of LDH bands. The gel photograph is a representative of 4 PAGE repeats.</p

Changes in LFT markers and ammonia in serum of the ALF rats.

<p>The data represents Mean ± S.D, where n = 4, **p<0.01, ***p<0.001 (control vs ALF group).</p

Level of active catalase declines in cerebral cortex and cerebellum of the ALF rats.

<p>Catalase activity measured in cell free extract (A) and non-denaturing PAGE pattern of active catalase (B and C). The technical details are same as described in Fig. 3 except 8% PAGE was used in case of B. *p<0.05; **p<0.01 (control vs ALF rats).</p

Changes in the levels of oxidative stress markers in cerebral cortex and cerebellum of the ALF rats.

<p>The data presents Mean ± S.D, where n = 4, *p<0.05, **p<0.01, (control vs ALF group).</p

Levels of ammonia and glutamate in cerebral cortex and cerebellum of the control and ALF rats.

<p>The data represents Mean ± S.D, where n = 4.</p

SOD activity shows differential pattern in cerebral cortex and cerebellum of the ALF rats.

<p>A represents SOD activity measured in cell free extracts; values are mean ± SD where n = 4 and each experiment done twice. In case of B, pooled tissue extracts from 4 rats containing 60 µg protein in each lane was electrophoresed on 12% non-denaturing PAGE followed by substrate specific development of SOD bands. The gel photograph in B is a representative of 4 PAGE repeats. In panel C, relative densitometric values of SOD band from experimental group, as % of the control lane, have been presented as mean ± SD from 4 PAGE repeats. *p<0.05; **p<0.01, ***p<0.001 (control vs ALF rats).</p