Morphological characterization of coriander genotypes

Poucas cultivares de coentro (Coriandrum sativum L.) estão disponíveis aos produtores e, em algumas regiões, cultivam-se genótipos de origem desconhecida, mantidos pelos próprios hortelãos. Para conservar as cultivares tradicionais é necessário não só coletá-las e conservá-las, mas também caracterizá-las. Dessa forma, este trabalho teve por objetivo determinar caracteres morfológicos para diferenciar genótipos de coentro no processo de registro de cultivares. Foram realizados dois experimentos, ambos em blocos casualizados, com cinco repetições. As cultivares Americano, Asteca, Palmeira, Português, Santo, Supéria, Tabocas, Tapacurá e Verdão, além da linhagem HTV-9299 foram analisadas para caracteres de fruto, plântula, folhas, folíolos e flores. Os dados obtidos foram submetidos à análise de variância univariada. A cultivar Palmeira apresentou frutos de maior tamanho e massa. Já para antocianina a cultivar Americano apresentou menor intensidade, ou praticamente ausência do pigmento tanto nas plântulas quanto nas plantas, ao contrário da cultivar Tabocas que apresentou maior intensidade. As cultivares Americano, Tabocas, Palmeira, Asteca, Verdão e Tabocas e a linhagem HTV-9299 possuem maior número de folhas basais, no entanto, as cultivares que produziram maior quantidade de massa por planta foram Tabocas, Palmeira, Verdão, HTV-9299 e Tapacurá. A cultivar Americano possui folíolo verde claro pouco amarelado, a linhagem HTV-9299 folíolo verde e a cultivar Tabocas folíolo verde escuro. Os descritores morfológicos com maior poder discriminatório para o grupo de dez cultivares avaliadas foram tamanho e massa de fruto; antocianina na plântula e planta; número de folhas basais; comprimento da quinta folha; tamanho e coloração de folíolo; número de dias para pendoamento e abertura da primeira flor e massa da planta fresca.Few coriander cultivars (Coriandrum sativum L.) are available to producers and, in some regions, are grown genotypes of unknown origin, kept by the growers themselves. To conserve such traditional crops it is necessary to collect, save, and characterize them. Thus, this study was to determine morphological characters to differentiate coriander genotypes aiming the registration of cultivars. Two trials were carried out, and the experimental design used in both was in completely randomized blocks with five replications. Americano, Asteca, Palmeira, Português, Santo, Superia, Tabocas, Tapacurá, and Verdão cultivars, and the line HTV-9299 were characterized for seedling, leaflets, leaves, fruits, and flowers. The data were subjected to a univariate ANOVA. The fruits of cv. Palmeira exhibited greater size and mass. As regards the presence of anthocyanins in seedlings and plants, cv. Americano showed the lowest intensity or slight pigmentation while cv. Tabocas had the highest pigmentation. The cultivars Americano, Palmeira, Asteca, Verdão, and Tabocas and the line HTV-9299 displayed higher number of basal leaves, however, the genotypes that produced larger amount of mass per plant were Tabocas, Palmeira, Verdão, HTV-9299, and Tapacurá. The green tone of leaflets showed differences among cultivars. So, cv. Americanos leaflets exhibited a light green, slightly yellowish color, HTV-9299 green, and Tabocas dark green. The morphological traits with higher discriminatory power for the group of the ten evaluated cultivars were: size and weight of fruits; presence or absence of anthocyanin in seedlings and plants; number of basal leaves; length of the fifth leaf; size and color of leaflets; number of days to bolting initiation, and opening of the first flower, and plant fresh mass

Genetic variability of half-sib progenies of coriander

Grande número de produtores envolve-se com o cultivo do coentro durante todo o ano, tornando a cultura importante social e economicamente. Praticamente em toda a região Nordeste utiliza-se a cultivar Verdão. Estudos da variabilidade genética do coentro são importantes, tendo em vista o melhor planejamento de programas de melhoramento genético. Desta forma, este trabalho teve como objetivo quantificar a variabilidade genética de características agronômicas do coentro cultivar Verdão, avaliando-se progênies de meios-irmãos potencialmente úteis no melhoramento genético. O trabalho foi desenvolvido na Universidade Federal Rural de Pernambuco, em casa de vegetação, em blocos casualizados, com cinco repetições e parcelas de 28 plantas, colocadas em dois vasos. Os tratamentos foram compostos por 55 progênies de meios-irmãos da cultivar Verdão. O teste t detectou significância a 1 e a 5% de probabilidade entre as correlações genotípicas, fenotípicas e ambientais. A herdabilidade no sentido amplo variou de 7,19 (peso médio de plantas) a 81,09 (número de plantas pendoadas). Para a razão entre os coeficientes de variação genético e ambiental (CVg /CVe), obteve-se de 0,27 (peso médio de plantas) a 2,07 (número de plantas pendoadas), indicando que a seleção contra o pendoamento apresenta as condições mais favoráveis em termos de ganhos genéticos imediatos. A correlação genotípica entre altura de plantas não pendoadas e número de plantas pendoadas foi alta e significativa.A large number of producers are involved in the cultivation of coriander throughout the year in Brazil, making it a crop of social and economic importance. Throughout nearly the entire Northeast Region, cultivar Verdão is used. Genetic variability studies on coriander are important for the proper planning of genetic improvement programs. The aim of the present study was to quantify the genetic variability for agronomic characteristics in cv. Verdão to contribute information toward genetic improvement. The study was developed at Universidade Federal Rural de Pernambuco, Pernambuco State, Brazil, in a greenhouse, using randomized blocks of 28 plants in two pots and five replications. The treatments were composed of 55 half-sib progenies from cultivar Verdão. The t test detected significance at 1 and 5% likelihood between the genotypic, phenotypic and environmental correlations. Broad sense heritability ranged from 7.19 for average plant weight to 81.09 for number of bolted plants. The ratio between genetic and environmental coefficients of variation (CVg/CVe) ranged from 0.27 (average weight) to 2.07 (number of bolted plants), indicating that selection against bolting presents more favorable conditions in terms of immediate genetic gains. The genotype correlation between height of non-bolted plants and the number of bolted plants was considered strong (0.86) and highly significant (t test), enabling simultaneous gains from selection

Early and Late Pathogenic Events of Newborn Mice Encephalitis Experimentally Induced by Itacaiunas and Curionópolis Bracorhabdoviruses Infection

In previous reports we proposed a new genus for Rhabdoviridae and described neurotropic preference and gross neuropathology in newborn albino Swiss mice after Curionopolis and Itacaiunas infections. In the present report a time-course study of experimental encephalitis induced by Itacaiunas and Curionopolis virus was conducted both in vivo and in vitro to investigate cellular targets and the sequence of neuroinvasion. We also investigate, after intranasal inoculation, clinical signs, histopathology and apoptosis in correlation with viral immunolabeling at different time points. Curionopolis and Itacaiunas viral antigens were first detected in the parenchyma of olfactory pathways at 2 and 3 days post-inoculation (dpi) and the first clinical signs were observed at 4 and 8 dpi, respectively. After Curionopolis infection, the mortality rate was 100% between 5 and 6 dpi, and 35% between 8 and 15 dpi after Itacaiunas infection. We identified CNS mice cell types both in vivo and in vitro and the temporal sequence of neuroanatomical olfactory areas infected by Itacaiunas and Curionopolis virus. Distinct virulences were reflected in the neuropathological changes including TUNEL immunolabeling and cytopathic effects, more intense and precocious after intracerebral or in vitro inoculations of Curionopolis than after Itacaiunas virus. In vitro studies revealed neuronal but not astrocyte or microglial cytopathic effects at 2 dpi, with monolayer destruction occurring at 5 and 7 dpi with Curionopolis and Itacaiunas virus, respectively. Ultrastructural changes included virus budding associated with interstitial and perivascular edema, endothelial hypertrophy, a reduced and/or collapsed small vessel luminal area, thickening of the capillary basement membrane, and presence of phagocytosed apoptotic bodies. Glial cells with viral budding similar to oligodendrocytes were infected with Itacaiunas virus but not with Curionopolis virus. Thus, Curionopolis and Itacaiunas viruses share many pathological and clinical features present in other rhabdoviruses but distinct virulence and glial targets in newborn albino Swiss mice brain