Selection of High-quality Sperms by the Nanotechnological Method of Magnetic Activation in Brazilian Cervids

Cervids show a high degree of abnormalities in their sperm cells. Thus, this study aimed to select high-quality spermatozoa using magnetic-activated sperm sorting (MASS) compared to density gradient centrifugation (DGC) by assessing the post-selection cell quality. Semen from six Mazama deer was collected by electroejaculation after chemical restraint. The semen was analyzed in four samples: Fresh, DGC, SEMgood - non-apoptotic fraction,and SEMpoor - apoptotic fraction. The material was analyzed for motility and vigor (light microscopy), concentration (Neubauer chamber), semen morphology (phase contrast), and supravital staining test (eosin/ nigrosine).The DGC method used 20 x 106 cells in 90% and 45% percoll® gradient.The MASS used 10 x 106 cells with 20 μl of iron nanoparticles attached to Annexin V and filtration in a magnetic separation column. Both processing methods (DGC and MASS) were effective in producing high-quality spermsamples, with a marked reduction in abnormalities from 41.83 ± 10.25 (fresh) to 14.83 ± 3.17(DGC) and 12 ± 3.01 (SEMgood), with 80.3% ± 2.06 livings cells. These findings suggest that this nanotechnological method,using nanoparticles, effectively produces high-quality semen samples in cervids for use in assisted reproduction

ANATOMIA ÓSSEA E MUSCULAR DO CÍNGULO ESCAPULAR E BRAÇO DE Tapirus terrestris (PERISSODACTYLA: TAPIRIDAE)

Tapirus terrestris (Linneaus, 1758) is a mammal found in South America and in almost all Brazilian biomes. The aim of this study was to describe the anatomy of bone and muscle of the scapular cingulum and arm of Tapirus terrestris and compare it with other species of mammals, especially equines. We used four animals donated to the Laboratory of Education and Research of Wild Animals of the Federal University of Uberlândia, after their death with no trauma. The bones were carefully analyzed, described and the muscles were dissected, analyzed and described in accordance with the usual techniques of gross anatomy. The skeleton of the scapular cingulum and arm of Tapirus terrestris is formed by scapula and humerus bones, the lateral muscles of the scapula are subclavian m., deltoid m. supraspinatus m. and infraspinatus, teres minor m., subscapularis m., teres major m., coracobrachialis m., shoulder joint m., biceps brachii m., brachial m. triceps, forearm tensor fasciae m., anconeus m. The muscular and bone standard found is similar to the horse (Equus caballus) T. terrestris has bone and muscle characteristics of a suitable animal to displacement and eventual swimming with obvious bone accidents and developed muscles