MOESM1 of Diversity of arsenite oxidase gene and arsenotrophic bacteria in arsenic affected Bangladesh soils

Additional file 1: Table S1. Primer sequences used for detection of bacterial 16S rRNA gene, arsenite resistance and oxidizing genes, and corresponding annealing temperature used for PCR. Fig. S1. PCR specific amplicon of 16S rRNA (a), arsenite resistance gene arsB (b) and arsenite oxidizing gene aoxB (c) of soil total DNA. Fig. S2. RFLP groups using AluI restriction digestion of aioA gene containing clones. Fig. S3. Different fragments obtained from Alu1 enzyme digestion of the 16S rRNA gene PCR product (approx. 1400–1450 bp) of arsenite resistant isolates. Representative groups are shown here. Uncut experimental DNA incubated under same condition was used as control. Marker used was 1 kb and 100 bp. Fig. S4. Amino acid sequence alignment (MEGA 5) of ten RFLP groups representative cloned arsenite oxidase genes from WFDSL-2 soil sample showing conserved catalytic motif. Fig. S5. Growth kinetics and corresponding oxidation of arsenite, As (III) to arsenate, As (V) by isolate (a) A1a (b) A1i

MOESM2 of Circulatory white spot syndrome virus in South-West region of Bangladesh from 2014 to 2017: molecular characterization and genetic variation

Additional file 2: Figure S1. Wu-Kabat protein variability index showed 13 unique mutations as observed from the protruded peaks’ positioned along with the amino acid residue of VP28 protein

MOESM1 of Circulatory white spot syndrome virus in South-West region of Bangladesh from 2014 to 2017: molecular characterization and genetic variation

Additional file 1: Table S1. qPCR profile of the studied samples by our designed and standardized method. Table S2. Accession no., sequence IDs, sources and collection time of the WSSVs sequences of dataset

MOESM4 of Circulatory white spot syndrome virus in South-West region of Bangladesh from 2014 to 2017: molecular characterization and genetic variation

Additional file 4: Figure S3. Ramachandran plot of modeled VP28 protein of WSSV. The number of residues in favored, allowed and outlier region are 96.4, 3.6 and 0%, respectively. On the X and Y axes, ϕ (phi) and ψ (psi) represent the torsion angles around Cα (alpha carbon) to amine and carboxyl group of different amino acids

MOESM3 of Circulatory white spot syndrome virus in South-West region of Bangladesh from 2014 to 2017: molecular characterization and genetic variation

Additional file 3: Figure S2. The aligned protein sequence data of VP28 taking into consideration 66 sequences generated by BioEdit Sequence Alignment Editor. The left portion of the frame contained the isolate ids of the sequences. The digit in the upper portion of the figure represented the residue number of protein. The red bar indicates the unique mutation position along with the mutated amino acid