Antiproliferative Activity and Constituents of Aspidosperma macrocarpon (Apocynaceae) Leaves

Aspidosperma macrocarpon belongs to the family Apocynaceae and is endemic to Americas and mainly found from Mexico to Argentina. It is known in Brazil as 'guatambu'or 'peroba'. Crude extracts and their fractions from leaves were assayed against human cancer cells lines: glioma (U251), melanoma (UACC-62), mammary (MCF-7), ovarian expressing the multidrug resistance phenotype (NCI-ADR/RES), lung (NCI-H460), prostate (PC-3), kidney (786-0), ovarian (OVCAR-3), colon (HT-29) and leukemia (K-562). The crude extract (EAM), hexane (HA) and chloroform (CA) fractions were the most active fractions against K-562 with GI50 values low than 1 mu g/mL. Also, CA was moderate active against OVCAR-3 and NCI-ADR/RES cells lines. This phytochemical study allowed to identify the known kopsanone, kopsinine, ursolic acid, rutin, 5-O-caffeoylquinic acid, and 3,5-O-dicaffeoylquinic acid. The kopsanone was also evaluated against human cancer cell lines and showed activity to the U251 and K-562 cell lines, with GI50 values of 20.6 mu g/mL and 8.7 mu g/mL, respectively.7213714

Cytotoxic activity of Guettarda pohliana Mull. Arg. (Rubiaceae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)The cytotoxic activity of crude extracts and their fractions from leaves and roots of G. pohliana was assessed against nine human cancer cell lines: melanoma (UACC-62), breast (MCF-7), breast expressing the multidrug resistance phenotype (NCI-ADR), lung (NCI-460), prostate (PCO-3), kidney (786-0), ovarian (OVCAR), colon (HT-29) and leukaemia (K-562). The hexane fraction from leaves (HL) and ethyl acetate (EAR), chloroform (CR) and hydromethanolic (HMR) fractions from roots were the most active fractions against K-562 with GI(50) values being lower than 1gmL(-1). Also, CR and HMR fractions were active against UACC-62 cell line in the same order of magnitude. The phytochemical study of the CR fraction allowed identifying the known iridoids secoxyloganin, sweroside and loganin.271816771681Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES