Statistical analysis of IL-1β −511 SNP.

<p>Statistical analysis of IL-1β −511 SNP.</p

Immunohistochemistry for IL-1β and <i>Leishmania mexicana</i> staining in lesions of patients with cutaneous leishmaniasis.

<p>(A) IL-1β staining on cells of LCL patient with single small lesion; (B) small clusters of <i>Leishmania</i> parasites in LCL patient with small ulcer; (C) diffuse distribution of IL-1β in LCL patient with abundant ulcers; (D) disintegrated <i>Leishmania</i> in LCL patient with abundant ulcers; (E) diffuse distribution of IL-1β in DCL patient; (F) clusters with abundant intact <i>Leishmania</i> parasites in DCL patient. Red arrows show IL-1β⁺ staining and black arrows show <i>L. mexicana</i> staining. (G) Normal skin was used as negative control for IL-1β immunostaining. (H) Control staining with secondary antibody. All sections were counterstained with haematoxylin. (A–H) scale bar = 50 µm. Immunostaining in tissue sections was visualized at a magnification of 400×. We show a representative result of different types of lesions within each group: LCL patients with one small ulcer: (n = 8 for IL-1β staining and n = 17 for <i>L. mexicana</i> staining) (A and B); LCL patients with various ulcers (n = 3) (C and D); DCL patients (n = 6) (E and F).</p

IL-1β expression in serum of patients with cutaneous leishmaniasis.

<p>(A) Western blot of mature IL-1β (17 kDa) in 4 controls (1–4), 9 LCL patients (5–13) and 7 DCL patients (14–20). (B) Graph of percentage intensity of IL-1β bands with statistically significant differences between controls <i>vs</i> LCL patients, controls <i>vs</i> DCL patients and LCL <i>vs</i> DCL patients. (Identical symbols above different bars are statistically significant: p<0.05).</p

Analysis of IL-1β cytokine production by non-stimulated monocytes purified with anti-CD14 magnetic beads from PBMC of control subjects and patients with cutaneous leishmaniasis after 18 hours <i>in vitro</i> culture.

<p>Mean values obtained from 7 healthy control subjects and 5 patients with leishmaniasis. (Identical symbols above two different bars are statistically significant: p<0.05).</p