Ascertainment of surfactin concentration in bubbles and foam column operated in semi-batch

This paper describes a mathematical model for the convection, diffusion, and balance phenomena for predicting the depletion curves, i.e., variations in the timed surface-active molecule concentration for fractionation processes in bubbles and foam column, operated in semi-batch. The model was applied for the purification of the surfactin solution and the results were compared with experimental data. Gibbs adsorption curves were obtained for the biosurfactant at different temperatures, and then adjusted with estimated parameters, according to the Langmuir adsorption model. The gas bubble sizes were optically determined. The isotherm adsorption parameters and bubble average diameter are crucial for the attainment of the depletion curves, generated by the model described. The results demonstrate that the process is most effective when operating a column with reduced gas flow and low initial concentration. A top product with two or thirty times greater concentration than the initial one was achieved and the highest biosurfactant concentrations were attained for higher operating temperatures. It was also observed that bubble diameter increased with a higher gas flow. The adjustment obtained for the adsorption curves of Gibbs was satisfactory. Therefore, there was evidence that surfactin molecules adsorb in monolayers in the liquid-gas interface.73COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESSem informaçã

Simulated Moving-bed Adsorption For Separation Of Racemic Mixtures

The two enantiomers that constitute a racemate have different activities when employed as pharmaceuticals. Due to this fact, fully recognized today, the pharmaceutical industry has been forced to market pure enantiomers instead of the racemic mixture whenever a chiral compound is involved. The simulated moving bed (SMB) is a chromatographic process that, unlike traditional HPLC systems, operates continuously without losing the enantiomeric purity of the outlet streams. The present work describes the enantioseparation of the anesthetic ketamine in a semipreparative-scale SMB unit. The chiral stationary phase employed was the microcrystalline cellulose triacetate. The outlet streams were analyzed by an on-line system, composed by an UV/VIS meter and a polarimeter, and also by HPLC. The analysis indicated purity values up to 100% for the stream of interest and up to 97.7% for the other stream.211127136Blaschke, G., Chromatographic resolution of chiral drugs on polyamides and cellulose triacetate (1986) Journal of Liquid Chromatography, 9 (2), pp. 341-368Cass, Q.B., Degani, A.L.G., Tiritan, E., Matlin, S.E., Curran, D.P., Balog, A., Enantiomeric resolution by HPLC of axial chiral amides using amylose tris((S)-1-phenylethylcarbamate) (1997) Chirality, 9, pp. 109-112Francotte, E.R., Wolf, R.M., Lohmann, D., Muller, R., Chromatographic resolution of racemates on chiral stationary phases. I. Influence of the supramolecular structure of cellulose triacetate (1985) Journal of Chromatography, 345, pp. 25-37Juza, M., Mazzotti, M., Morbidelli, M., Simulated moving bed and its application to chiraltechnology (2000) Trends in Biotechnology, 18, pp. 108-118Lodevico, R.G., Bobbit, D.R., Edkins, T.J., Determination of enantiomeric excess using a chiral selective separation mode and polarimetric detection (1997) Talanta, 44, pp. 1353-1363Mazzotti, M., Storti, G., Morbidelli, M., Optimal operation of simulated moving beds for nonlinear chromatographic separations (1997) Journal of Chromatography A, 769, pp. 3-24McCoy, M., SMB emerges as a chiral technique (2000) Chemical and Engineering News, 78 (25), pp. 1-3Nicoud, R.M., A packing procedure for high flow rate and high stability columns using cellulose triacetate (1993) LC - GC Int., 6, pp. 636-637Nicoud, R.M., The separation of optical isomers by simulated moving bed chromatography (Part II) (1999) Pharmaceutical Technology Europe, 11, pp. 28-34Pedeferri, M.P., Zenoni, G., Mazzotti, M., Morbidelli, M., Experimental analysis of a chiral separation through simulated moving bed chromatography (1999) Chemical Engineering Science, 54, pp. 3735-374