Additional file 1: of Suicide prevention gatekeeper training in the Netherlands improves gatekeepers’ knowledge of suicide prevention and their confidence to discuss suicidality, an observational study

Questionnaire Gatekeeper training. Data identifies people at risk of suicide, referral to GP, referral to 113 Suicide Prevention, knowledge and confidence. Personal data of participants and ordinal data on a 5 point scale. (DOCX 15 kb

Reduced chemokine production in the lung of Myd88^-/- mice during infection with D39 or D39Δ<i>cps</i>.

<p>KC/CXCL1 (A and B) and MIP2/CCL2 (C and D) of wild type (WT, black bars) and Myd88^-/- mice (MyD88 KO, white bars) at the indicated time points after inoculation with D39 (A/C) or at 6 or 24 hours post inoculation D39Δ<i>cps</i> (B/D). Data are mean ± SEM (N = 6–8 mice per group at each time point). ***P<0.0005 vs WT mice.</p

Reduced neutrophil infiltration in the lung in Myd88 ^-/- mice during infection with D39 or D39Δ<i>cps</i>.

<p>Representative Ly6-C/G stainings of lung sections obtained 6 h after induction of pneumococcal pneumonia with D39 in WT and Myd88^-/- mice (A and B) and with D39Δ<i>cps</i> in WT mice and Myd88^-/- mice (E and F). Original magnification 20x. Percentage lung area with Ly6-C/G-positive neutrophils was quantified by digital image analysis as described in methods section for wild type (WT, black bars) and Myd88^-/- mice (MyD88 KO, white bars)(C and G). Levels of MPO in lung homogenates of wild type (WT, black bars) and Myd88^-/- mice (MyD88 KO, white bars) inoculated with D39 at 6 and 48 hours after challenge, or inoculated with D39Δcps (D and H) at 6 and 24 hours after challenge were determined by ELISA. Data are mean ± SEM (N = 5–8 mice per group at each time point). *P<0.05, ***P<0.0005 vs WT mice.</p