Selective flotation of sphalerite and galena in the presence of unfractionated or thermolysed and fractionated components of <i>B.circulans.</i>

<p>a - normal cells; b - thermolysed cells; c - thermolysed cell-free supernatant; d - thermolysed cell pellet; e – DNase 1 treated thermolysed cell-free supernatant. The numbers above the bar chart indicates the selectivity index (S.I) values.</p

Schematic diagram of the amphipathic nature of ssDNA.

<p>(A) Amphipathic structure of ssDNA indicating the hydrophobic and the hydrophilic faces; (B) - Effect of spermidine concentration on the ssDNA mediated flotation of sphalerite; (C) - Effect of depurination of DNA on the flotation of sphalerite a - dsDNA; b - depurinated dsDNA; c - ssDNA; d - depurinated ssDNA.</p

Effect of the strandedness of DNA on the flotation of sphalerite.

<p>a - thermolysed cell-free supernatant; b - genomic DNA (2 mg of dsDNA); c – DNase 1 treated genomic DNA; d - ssDNA (2 mg); e - DNase 1 treated ssDNA.</p

Flotation recovery of sphalerite and galena as a function of the ssDNA concentration.

<p>Flotation recovery of sphalerite and galena as a function of the ssDNA concentration.</p

Effect of buffering of cells on the flotation recovery of sphalerite and galena.

<p>Effect of buffering of cells on the flotation recovery of sphalerite and galena.</p

Flotation recovery of sphalerite and galena during reconstitution of the selective flotation of sphalerite with a fixed amount (2

<p> <b>mg) of ssDNA and varying amounts of DNase 1 treated non-DNA components of cells.</b></p

Effect of rapamycin on SCC tumor growth and mTOR signaling pathway.

<p>(A) Tumor sizes of the SCC tumors in the mice leg treated with vehicle (control, •), 5 mg/kg bw/day (▴), and 10 mg/kg bw/day (▪) rapamycin. (B) Western blot analysis of S6 protein expression and the abundance of its phosphorylated form in SCC tumor cells treated with rapamycin (100 nM). (C, D) Immunostaining of pS6 in SCC xenograft of control and rapamycin treated (10 mg/kg bw/day, 2 days).</p

Effect of combination of rapamycin and X-irradiation on tumor growth.

<p>Growth kinetics of SCC tumors implanted in mice leg, treated with vehicle (control, •), X-irradiation (5 Gy/day, 3 days, □), rapamycin (10 mg/kg/day, 5 days, ▴), and rapamycin and X-irradiation (◊).</p

Anatomy, pO₂, and blood volume images of SCC tumor.

<p>T₂-weighted anatomical image (top) of a SCC tumor-bearing mouse, and the corresponding pO₂ maps (middle) and blood volume images (bottom) measured by EPRI and MRI. The adjacent center six slices of the 3D images were displayed, and the every slice has 2 mm thickness.</p

Changes in tumor oxygenation and blood volume.

<p>Median pO₂ values (A), percentage of hypoxic fraction (B), and mean blood volume (C) in the control and rapamycin treated SCC tumors. The values are average of 5 or 6 mice and error bars represent standard deviations. Oxygen delivery per unit blood volume (D) was calculated by dividing tumor pO₂ by blood volume. * p<0.05 as compared with control, ** p<0.01 as compared with control, † p<0.05 as compared with rapamycin day 8, †† p<0.005 as compared with rapamycin day 8.</p