Comparison of the sensitivity, specificity and likelihood ratio of tests.

<p>p-ELISA = enzyme immunoassay with antigens of promastigotes of <i>Leishmania</i> sp. DAT = direct agglutination test, IFAT = indirect immunofluorescence; rK39-ELISA = enzyme immunoassay with rK39 antigen, n = number of studies included in the analysis, S = sensitivity, Sp = specificity; LR+ = positive likelihood ratio, LR− = negative likelihood ratio. CI = confidence interval.</p><p>*Analysis in which the study by Romero et al. was excluded because of discrepancies noted in the published data.</p

ROC plot comparing the performance of the rK39 strip test to serologic techniques utilizing promastigote antigens for diagnosis of Visceral Leishmaniasis.

<p>ROC plot comparing the performance of the rK39 strip test to serologic techniques utilizing promastigote antigens for diagnosis of Visceral Leishmaniasis.</p

ROC plot comparing the performance of the rK39-ELISA to the p-ELISA and IFAT for the diagnosis of Visceral Leishmaniasis.

<p>(<b>A</b>) Analysis including all studies that met our selection criteria. (<b>B</b>) Analysis after removal of the outlier study.</p

ROC plot showing the heterogeneity of strip test by continent.

<p>ROC plot showing the heterogeneity of strip test by continent.</p

Algorithm for selection of studies.

<p>Algorithm for selection of studies.</p

Time-scaled phylogeographic reconstruction of the 259 HIV-1 sequences from South-East Austria.

<p>Ancestral nodes are colored according to the inferred most probable location state with the color codes corresponding to the urban areas (Graz City and suburban Graz) in red and the rural areas in blue. Branch colors represent the most probable location of the parental node of each branch. Tips are colored according to the observed sampling location of each sequence. Each clade is highlighted in unique color: green (subtype B), red (subtype F), blue (CRF02_AG), gray (subtype D), orange (Subtype C) and purple (CRF01_AE).</p

HIV Subtype in the South-East Austrian Cohort.

<p><b>2A</b>. Comparison between clustering and non-clustering populations by HIV subtype. <b>2B</b>. HIV Subtype by Risk factors in the South-East Austrian Cohort. Legend: HSX, heterosexual contact; IDU, injection drug use; MSM, men who have sex with men; MTCT, mother to child transmission; OTH, other.</p

Baseline Demographic and Viral Characteristics.

<p>Baseline Demographic and Viral Characteristics.</p

Intersection between the Global HIV Type 1 Endemic and South East Austria.

<p>A total of 107 (41.3%) of 259 sequences obtained in South-East Austria shared a putative link with sequences from foreign countries/regions. In this heat map with a gradient from 1 to 40 linkages, countries are colored by the number of putative links from yellow to red (i.e. red is 40 or more linkages). Unlinked countries are colored in white.</p

Maximum likelihood phylogenetic trees (by subtype).

<p>All clusters identified by network analysis were supported by a bootstrap value above 80%. The bootstrap value of the larger clusters is indicated in blue at the root of each corresponding clusters and non-dyad clusters are highlighted in grey with the cluster size indicated for each cluster. Tips are colored by their reported risk factor in red (men who have sex with men, MSM), green (heterosexual, HSX), blue (injection drug use, IDU), purple (MSM plus IDU) and grey (unknown/others), respectively. All trees (Subtype B, CRF_01AE, CRF_02AG and subtype F) include a set of reference consensus sequences from the main subtypes and CRF forms, and a set of reference sequences from the same subtype.</p