Various features of PLC genes in rice genome.

<p>Various features of PLC genes in rice genome.</p

Co-localization of rice NPC proteins with chlorophyll auto-fluorescence of the cell.

<p>Confocal images of <i>Nicotiana benthamiana</i> cell expressing GFP-OsNPC1 (upper panel) and GFP-OsNPC3 (lower panel). Green GFP signal merges with red auto-fluorescence of chloroplast, as seen in the merged regions (yellowish orange) in the overlay. All the images were taken in 5 different sections in z direction and merges together. Scale bar = 40 µm.</p

Expression profile of rice PLC gene family during developmental stages.

<p>Average signal intensity (GCRMA) value of three replicates from microarray for all the developmental stages (three vegetative; L-mature leaf, R-root, SDL-seven day old seedling, six panicle stages; P1–P6 and five seed stages; S1–S5) has been plotted to show the differential expression. Standard error bars have been shown. Y-axis represents signal values from microarray and X-axis shows different developmental stages.</p

Phylogenetic relationship of rice and <b><i>Arabidopsis</i></b><b> PLC gene family.</b>

<p>An un-rooted neighbour joining phylogenetic tree was constructed from the protein sequences of rice and <i>Arabidopsis</i> PLCs, including both PI-PLCs and NPCs. Multiple sequence alignment was carried out using clustalX2.0 and the tree was generated using MEGA5. Rice and <i>Arabidopsis</i> PI-PLCs and NPCs are clustered together based on significant bootstrap value (>50%). Scale bar indicates 0.1 amino acid substitution per site.</p

Subcellular localization of OsPLC proteins in <b><i>Nicotiana benthamiana</i></b><b> cells.</b>

<p>Agrobacterium-infiltrated tobacco leaves expressing the GFP-PLC fusion protein driven by the 2XCaMV 35S promoter. Confocal images of fluorescence (green) for cell expressing OsPLC1 and OsPLC4 are showing their distribution throughout the cytoplasm and nucleus (first and second row). Expressed GFP-OsNPC1 fusion protein, mainly localized to cytoplasm and small spots like structures in the cell (third row), and GFP-OsNPC3 fusion protein showed preferential localization to small punctate structures in the cell (fourth row). Cells transformed with vector only (CaMV35S-GFP) are shown in the lowermost row. All the images were taken in 5 different sections in z direction and merges together. Scale bar = 40 µm.</p

MADS-box gene family in rice: genome-wide identification, organization and expression profiling during reproductive development and stress-2

<p><b>Copyright information:</b></p><p>Taken from "MADS-box gene family in rice: genome-wide identification, organization and expression profiling during reproductive development and stress"</p><p>http://www.biomedcentral.com/1471-2164/8/242</p><p>BMC Genomics 2007;8():242-242.</p><p>Published online 18 Jul 2007</p><p>PMCID:PMC1947970.</p><p></p>motif is represented by a number in colored box. Length of box does not correspond to length of motif. Order of the motifs corresponds to position of motifs in individual protein sequence. For detail of motifs refer to supplementary material

MADS-box gene family in rice: genome-wide identification, organization and expression profiling during reproductive development and stress-8

<p><b>Copyright information:</b></p><p>Taken from "MADS-box gene family in rice: genome-wide identification, organization and expression profiling during reproductive development and stress"</p><p>http://www.biomedcentral.com/1471-2164/8/242</p><p>BMC Genomics 2007;8():242-242.</p><p>Published online 18 Jul 2007</p><p>PMCID:PMC1947970.</p><p></p>Y-axis represents the raw expression values obtained using microarrays. Chromosome numbers on the left of each graph represent the duplicated segments on which the duplicated genes were identified. Homology for each duplicated gene pair is given in brackets

MADS-box gene family in rice: genome-wide identification, organization and expression profiling during reproductive development and stress-0

<p><b>Copyright information:</b></p><p>Taken from "MADS-box gene family in rice: genome-wide identification, organization and expression profiling during reproductive development and stress"</p><p>http://www.biomedcentral.com/1471-2164/8/242</p><p>BMC Genomics 2007;8():242-242.</p><p>Published online 18 Jul 2007</p><p>PMCID:PMC1947970.</p><p></p>are written at the top. Genes belonging to five groups have been marked by different colors (MIKC, red; MIKC*, yellow; Mα, purple; Mβ, green and Mγ, blue). Genes lying on duplicated segments of genome have been joined by dashed lines. Tandemly duplicated genes are joined with vertical lines. (* The duplicated segment between chromosome 1 and 9 containing and , respectively, could be detected only in segmental duplication database with 500 kb and not with 100 kb distance allowed between collinear genes in TIGR, for details experimental procedures)

MADS-box gene family in rice: genome-wide identification, organization and expression profiling during reproductive development and stress-6

<p><b>Copyright information:</b></p><p>Taken from "MADS-box gene family in rice: genome-wide identification, organization and expression profiling during reproductive development and stress"</p><p>http://www.biomedcentral.com/1471-2164/8/242</p><p>BMC Genomics 2007;8():242-242.</p><p>Published online 18 Jul 2007</p><p>PMCID:PMC1947970.</p><p></p>Differential expressions shown by seven MADS-box genes in response to various abiotic stress conditions. Left panel shows four genes up regulated and right panel shows down regulated genes more than 2 folds with p value less than 0.05 in response to three abiotic stress conditions. X-axis represents seedling followed by stress samples (CS, cold stress; DS, dehydration stress; SS, salt stress). Y-axis represents average expression values obtained using microarrays. Error bars represent standard error for data obtained in three biological replicates

Promoter analysis of rice PLC gene family.

<p><i>Cis</i>-regulatory elements in the 1 kb upstream region (from translation start site) of the promoter of all the rice PLC genes were analysed using PlantCARE database. Various elements such as ABRE, MBS, TC-rich repeat, C-repeat/DRE, RY-elements, Skn-1 and GCN4 are present in the forward and reverse strands and are indicated by the arrowhead mark above and below the line, respectively. Scale over the promoters indicates the specific location of the elements. Differential regulation (up- or down-regulation) of the genes under different abiotic stresses; S-salt, C-cold, D-drought and during reproductive developmental phases; P- panicle and Sd- seed, is indicated on the right side of each gene.</p