48 research outputs found

    Expression and Characterization of Chandipura Virus Proteins

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    Chandipura virus (CHPV) has recently emerged as an extremely lethal human pathogen in the family Rhabdoviridae and is linked to significant encephalitis outbreaks in different parts of India. The biology of CHPV remains less studied to date and the availability of reagents such as purified proteins can enhance research in this direction. In this study, we have overexpressed four of the CHPV proteins namely Nucleoprotein (N), Phosphoprotein (P), Matrix protein (M) and Glycoprotein (G) using three  distinct tags in bacterial system and with changes in inducer concentration, growth and solubilisation conditions successfully purified M and G proteins for the first time along with N and P. Furthermore, the interactions of CHPV M protein with other viral proteins (G, N and P) was investigated using ELISA and GST pull down assays to show the utility of olubilised proteins. The results of both the assays demonstrated that M protein interacts with both G and N proteins, while it does not interact with P protein, in a similar  manner as reported for Vesicular Stomatitis Virus

    Anti-tuberculosis drug induced hepatotoxicity: a study from Himalayan region

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    Background: Tuberculosis (TB) is the infection of global health concern. The management of TB is a 6-month course of anti-TB drugs. Compliance is crucial for curing TB. Adverse effects often affect the compliance negatively. One of the adverse effects affecting TB treatment outcome is anti-TB drug induced hepatotoxicity (DIH). Therefore, the purpose of this study was to assess the incidence of anti-TB DIH and its associated factors among newly diagnosed TB patients.Methods: A single centre prospective study was conducted from January-December 2020. All patients who were newly-diagnosed for TB, started anti-TB medication and diagnosed with drug-induced liver injury during anti-tubercular treatment included in the study.Results: Total of four hundred and ninety-two (492) TB patients taking anti-TB drugs were involved in this study with male predominance and maximum in the age group of 30-45 years. Smear-positive pulmonary TB accounted for 66.9% of all cases. During the study period, 9.3% TB patients developed anti-TB DIH. Among the cases of anti-TB DIH, female patients account for 52%. Patients with extra-pulmonary TB (n=23), low BMI (n=16), alcohol consumption (n=21) had developed anti-TB DIH. The time interval from the initiation of treatment to the onset of hepatotoxicity was 16-45 days.Conclusion: The chances of hepatotoxicity among TB patients taking anti-TB drugs are always there. Thus, it is necessary to monitor liver function in patients receiving anti-TB drugs routinely

    Antioxidant capacity and combinatorial antimicrobial effects of Nardostachys jatamansi essential oil with conventional antibiotics against some drug resistant bacteria

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    The antibacterial and antioxidant properties of essential oils (EOs) have long been recognized. The present study was conducted to investigate the antioxidant capability of Nardostachys jatamansi essential oil and to see if it has a synergistic antimicrobial effect with antibiotics against two Gram negative (Klebsiella pneumoniae and Escherichia coli) and three Gram positive (Bacillus subtilis, Micrococcus luteus and Staphylococcus aureus) bacterial strains. Guaia-6,9-diene (11.96 %), calarene (10.44 %), jatamansone (8.11 %), α-gurjunene (7.42 %), valencene (6.46 %), α-maaliene (5.24 %), sprojatamol (5.06 %), and caratol (5.06 %) were found to be the primary components of N. jatamansi EO. According to antioxidant studies, N. jatamansi EO has moderate DPPH radical scavenging activity, reducing power, and ferric reducing antioxidant power. Similarly, N. jatamansi EO also showed significant antibacterial activity, with inhibition zones, MIC, and MBC values ranging from 10.5 ± 0.5 to 14.0 ± 0.4 mm, 1.5 to 3.1 mg/mL, and 1.8 to 3.5 mg/mL respectively. The results of N. jatamansi EO interactions with conventional antibiotics revealed that amoxicillin, erythromycin, chloramphenicol, and ampicillin MICs were reduced by 5 to 10 fold, 4 to 9.09 fold, 4 to 10.5 fold, and 4 to 8.0 fold, respectively. The findings of this study are noteworthy because no previous reports of N. jatamansi EO's synergistic interaction with conventional antibiotics have been published, and therefore may constitute an important strategy for addressing problem of drug resistant bacteria

    Antioxidant and Antimicrobial Properties of the Essential Oil and Extracts of Zanthoxylum alatum

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    The essential oil obtained from the fresh leaves of Zanthoxylum alatum was analysed by gas chromatography/mass spectrometry (GC/MS). Fourteen components were identified, and linalool (30.58%), 2-decanone (20.85%), β-fenchol (9.43%), 2-tridecanone (8.86%), β-phellandrene (5.99%), Sabinene (4.82%), and α-pinene (4.11%) were the main components. The EO and methanolic extract of Z. alatum exhibited potent antifungal activity against Alternaria alternata, Alternaria brassicae, and Curvularia lunata. The EO also showed significant antibacterial activity against Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus, and Escherichia coli. Further, antimicrobial constituents of the EO were isolated by bioautography and preparative thin layer chromatography (PTLC) and identified as β-fenchol and linalool using GC/MS analysis. In addition to this, the free radical scavenging activity and antioxidant potential of EO and methanolic extract/fractions of Z. alatum were also investigated using in vitro assays including scavenging ability against DPPH•, reducing power and chelating ability on Fe2+ ions. Our results demonstrate that Z. alatum could be used as a resource of antioxidant and antimicrobial compounds which may find applications in food and pesticide industries

    Nations within a nation: variations in epidemiological transition across the states of India, 1990–2016 in the Global Burden of Disease Study

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    18% of the world's population lives in India, and many states of India have populations similar to those of large countries. Action to effectively improve population health in India requires availability of reliable and comprehensive state-level estimates of disease burden and risk factors over time. Such comprehensive estimates have not been available so far for all major diseases and risk factors. Thus, we aimed to estimate the disease burden and risk factors in every state of India as part of the Global Burden of Disease (GBD) Study 2016

    Antimicrobial Activity of Some Essential Oils—Present Status and Future Perspectives

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    Extensive documentation on the antimicrobial properties of essential oils and their constituents has been carried out by several workers. Although the mechanism of action of a few essential oil components has been elucidated in many pioneering works in the past, detailed knowledge of most of the compounds and their mechanism of action is still lacking. This knowledge is particularly important for the determination of the effect of essential oils on different microorganisms, how they work in combination with other antimicrobial compounds, and their interaction with food matrix components. Also, recent studies have demonstrated that nanoparticles (NPs) functionalized with essential oils have significant antimicrobial potential against multidrug- resistant pathogens due to an increase in chemical stability and solubility, decreased rapid evaporation and minimized degradation of active essential oil components. The application of encapsulated essential oils also supports their controlled and sustained release, which enhances their bioavailability and efficacy against multidrug-resistant pathogens. In the recent years, due to increasingly negative consumer perceptions of synthetic preservatives, interest in essential oils and their application in food preservation has been amplified. Moreover, the development of resistance to different antimicrobial agents by bacteria, fungi, viruses, parasites, etc. is a great challenge to the medical field for treating the infections caused by them, and hence, there is a pressing need to look for new and novel antimicrobials. To overcome these problems, nano-encapsulation of essential oils and exploiting the synergies between essential oils, constituents of essential oils, and antibiotics along with essential oils have been recommended as an answer to this problem. However, less is known about the interactions that lead to additive, synergistic, or antagonistic effects. A contributing role of this knowledge could be the design of new and more potent antimicrobial blends, and understanding of the interplay between the components of crude essential oils. This review is written with the purpose of giving an overview of current knowledge about the antimicrobial properties of essential oils and their mechanisms of action, components of essential oils, nano-encapsulated essential oils, and synergistic combinations of essential oils so as to find research areas that can facilitate applications of essential oils to overcome the problem of multidrug-resistant micro-organisms

    Antioxidant activity of acetone extract/fractions of <i>Terminalia bellerica </i>Roxb. fruit

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    110-116Terminalia bellerica Roxb. (Family: Combretaceae) has been valued in Indian system of medicine for treatment of wide range of diseases and reported to have antioxidant properties. In the present study, the free radical scavenging activity and antioxidant potential of acetone extract/fractions of its fruit was investigated using in vitro assays, including scavenging ability against 2,2′-diphenyl-2-picrylhydrazyl (DPPH), β-carotene bleaching inhibition, reducing power and chelating ability on Fe2+ ions. The fruit powder was extracted at room temperature with different solvents in the order of increasing and decreasing polarity to obtain crude acetone extract which was further partitioned with ethyl acetate and water (1:1). It was found that ethyl acetate fraction was more effective than crude acetone extract in all antioxidant assays, except chelating power which was highest in water fraction. Maximum antioxidant activities (expressed as EC50 values) observed were 14.56 μg/ml, 27.81 μg/ml and 67.8 μg/ml in DPPH, β-carotene bleaching and reducing power assays, respectively. The antioxidant potential was compared with known antioxidant (butylated hydroxyl toluene) and correlated with total phenolic and flavonoid content in crude extract and fractions. Fractions rich in polyphenolic content were more effective than the crude extract
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