Particle size distributions of chitosan-LHRH (A) and chitosan-gold-LHRH nanoparticles (B) and their respective TEM Images (a, b).

<p>Particle size distributions of chitosan-LHRH (A) and chitosan-gold-LHRH nanoparticles (B) and their respective TEM Images (a, b).</p

Formation of chitosan gold nanoparticles. Varying the ratio of gold salt to chitosan in the medium helps in achieving size controlled synthesis of nanoparticles.

<p>Nanoparticles formed in the last beaker were used for animal experimentation.</p

Schematic diagram showing the functional structure of chitosan encapsulated gold nanoparticles and subsequent conjugation of LHRH on chitosan encapsulated gold nanoparticles.

<p>Schematic diagram showing the functional structure of chitosan encapsulated gold nanoparticles and subsequent conjugation of LHRH on chitosan encapsulated gold nanoparticles.</p

Histology of ovary from <i>Cyprinus carpio</i> injected with different preparations of salmon luteinising hormone- releasing hormone (LHRH).

<p>A]. from control fishes: 1.Ovigerous lamellae with immature oocyte; 2. mature oocyte; 3. maturing oocyte; 4. atretic oocyte. B] from fish injected with bare LHRH. 1. mature/ripe oocyte; 2. Partially spent oocyte; 3. cortical alveoli stage oocyte; 4. spent follicle; 5. immature oocytes in nest C] from fish injected with chitosan LHRH nanoparticles. 1. Oocytes nested in lamellae 2. spent follicle 3. spent oocytes 4. ovigerous lamellae with immature oocytes D] from fish injected with chitosan gold LHRH nanoparticles 1. mature oocytes 2. spent oocytes 3. immature oocytes 4. discharged follicle.</p

Schematic diagram of the functional structure of chitosan nanoparticles formed during ionic gelation process.

<p>(A) Sodium tripolyphosphate, (B) chitosan, (C) chitosan nanoparticles.</p

Reproductive output of the <i>Cyprinus carpio</i> injected with different preparations of salmon luteinising hormone-releasing hormone (LHRH).

<p>Percent relative eggs and fertilised eggs are expressed as relative to 100% in bare LHRH preparation. Fishes were injected with bare LHRH, chitosan LHRH nanoparticles (ChLHRHNPS) and chitosan-gold LHRH (ChAuLHRHNPs).</p

Morphological and physico-chemical characteristics of salmon luteinizing-releasing hormone (LHRH) nanoparticles at 25°C in diluent water.

†<p>Size and zeta potential are expressed as mean± SE of n = 3.</p>*<p>P<0.05 (by student t test).</p

Serum levels of various reproductive hormones in female <i>Cyprinus carpio</i> following injection of different preparations of salmon luteinising hormone-releasing hormone (LHRH) during 24 hr period.

<p>Fishes were injected with either no active compounds (control) or with bare LHRH, chitosan LHRH nanoparticles (ChLHRHNPs) and chitosan-gold LHRH (ChAuLHRHNPs). Means bearing different superscript letters (a, b, c, d) differ significantly (P<0.01) at that hour point (vertical comparison). Each point is mean ±SE of three observations.</p