Sensitivity of the PCR assay in confirmed VL cases.

<p>Sensitivity of the PCR assay in confirmed VL cases.</p

PCR result for spiked samples.

<p>Lane 1–3: 10,000 parasites, Lane 4–6: 1000 parasites, Lane 7–9: 100 parasites, Lane 10–12: 10 parasites, Lane 13–15: 1 parasite, Lane 16–18: No Template controls (NTC), M = 100 bp marker.</p

Sequence alignment and primer designing.

<p>Alignment of the DNA sequence within the 18S rRNA gene of <i>Leishmania donovani</i> (<i>L.d.</i>; GenBank accession no. X07773), <i>Trypanosoma brucei gambiense</i> (<i>T.b.</i>; GenBank accession no. AJ009141), and <i>Trypanosoma cruzi</i> (<i>T.c.</i>; GenBank accession no. AF303660). The forward (BHUL18SF) and reverse (BHUL18SR) primers are indicated by arrows.</p

PCR results in clinical samples.

<p>Lanes H1and H2: Non-endemic healthy controls, Lane N1 and N2: negative controls, Lanes P1 to P8: VL patients, M = 100 bp marker.</p

Comparative specificity in different control groups.

<p>Comparative specificity in different control groups.</p

Sanger sequencing chromatograms for validation of genotypes.

<p>A) Homozygous CC genotype B) Heterozygous CT genotype C) Homozygous TT genotype.</p

The genotype distribution of -1562C>T MMP9 gene polymorphisms in patients with open-angle glaucoma (POAG) and angle closure glaucoma (PACG) according to CDR and IOP.

<p>The genotype distribution of -1562C>T MMP9 gene polymorphisms in patients with open-angle glaucoma (POAG) and angle closure glaucoma (PACG) according to CDR and IOP.</p

Distribution of allele frequency, genotype frequency and genetic model of rs3918242 among PACG cases and controls.

<p>Distribution of allele frequency, genotype frequency and genetic model of rs3918242 among PACG cases and controls.</p

Gender-wise comparison of POAG cases and controls.

<p>Gender-wise comparison of POAG cases and controls.</p

Frequency distribution of males and females among POAG and PACG cases with respect to control subjects.

<p>Frequency distribution of males and females among POAG and PACG cases with respect to control subjects.</p