Ntr1p overabundance affects NHEJ of linearized plasmid DNA transformed into yeast, or of chromosomal DNA double-strand breaks in different genetic backgrounds

<p><b>Copyright information:</b></p><p>Taken from "Conserved interactions of the splicing factor Ntr1/Spp382 with proteins involved in DNA double-strand break repair and telomere metabolism"</p><p></p><p>Nucleic Acids Research 2007;35(7):2321-2332.</p><p>Published online 27 Mar 2007</p><p>PMCID:PMC1874655.</p><p>© 2007 The Author(s)</p> () Wild-type (wt) or NHEJ-deficient Δ yeast strains constitutively producing full-length EGFP-tagged-Ntr1p from plasmid pUG36 (vector control) were transformed with equal amounts of digested or undigested plasmid pBTM116, which is a substrate for NHEJ (). Results are presented as relative transformation efficiencies (ratios of cut:uncut plasmid). EcoRI cut indicates 5′-overhangs, PstI cut indicates 3′-overhangs. Error bars represent one standard deviation, statistical significance (-values) by a two-tailed students -test is indicated. () Wild-type (wt), HR-deficient Δ, or NHEJ-deficient Δ strains constitutively producing full-length Ntr1p from plasmid pAS2–1. Chromosomal breaks were induced by additional expression of EcoRI or HO (GAL1-inducible) upon transformation of the respective expression vectors (). Results are presented as percentage of survival of cells carrying an NTR1-expressing vector or the respective control vector (pAS2-1) when grown on galactose containing medium. Error bars represent one standard deviation, -values of a two-tailed students -test are indicated

Yeast and human NTR1 co-localize with nucleolus- and telomere-associated proteins

<p><b>Copyright information:</b></p><p>Taken from "Conserved interactions of the splicing factor Ntr1/Spp382 with proteins involved in DNA double-strand break repair and telomere metabolism"</p><p></p><p>Nucleic Acids Research 2007;35(7):2321-2332.</p><p>Published online 27 Mar 2007</p><p>PMCID:PMC1874655.</p><p>© 2007 The Author(s)</p> () Intracellular localization of Ntr1p and co-localization with other proteins. Upper panel: EGFP-Ntr1p (green) localizes to the nucleus (DAPI, light blue) and forms foci. Middle panel: live cell images of CFP-Nop1p (red, false color) and EGFP-Ntr1p (green). Co-localizing signals are shown in yellow in the merge panel. Lower panel: confocal images of EGFP-Ntr1p (green) in fixed cells immunostained for Rap1p (red). DAPI staining of DNA is shown in blue. Co-localization between the two proteins is shown in yellow on the merge panel. () Intracellular localization of human NTR1 and TRF1. WI26 VA4 cells were co-transfected with expression constructs of ECFP-NTR1 (amino acids 289–580) and RFP-TRF1 (upper three panels). eCFP was used as a control (lower panel). Co-localization in confocal images is shown in yellow on the merge panel and telomeric co-localization is indicated by arrows