Description of a new species, Pintomyia dissimilis nov. sp., a phlebotomine fossil from Dominican Republic amber (Diptera: Psychodidae: Phlebotominae)

<p>Abstract</p> <p>Background</p> <p>Phlebotomine sandflies are the vectors of etiological agents of leishmaniases in several areas of the world. In the Neotropical Region, the biodiversity of these insects is more than other regions, probably due the long evolutionary period of this group. Miocene amber from Dominican Republic, currently, has a record of 14 extinct species of Phlebotomine sandflies.</p> <p>Results</p> <p>This paper describes a new fossil species of phlebotomine sandfly from amber found in Dominican Republic. This new species is based on morphological characters of a male such as 5° palpomere longer than 3° + 4°, three well-developed spines in the gonostyle, lateral lobe longer than gonocoxite and permit inclusion of the new species in the genus <it>Pintomyia</it>, series <it>serrana</it>. The paramere, with a curvature in the ventral margin, of the middle of the structure, separates the new species from the others fossils or extant species.</p> <p>Conclusion</p> <p>The new species described in the present study named <it>Pintomyia dissimilis </it>nov. sp. is well differenciated from all known species in this genus.</p

Leishmaniasis in the municipality of Palma Lowland, Minas Gerais, Brazil: A study of canine leishmaniasis and sandflies

Submitted by Repositório Arca ([email protected]) on 2019-05-07T13:26:25Z No. of bitstreams: 2 license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) cristiani_castilho.pdf: 1486413 bytes, checksum: c013a7bd4c83b4837986dd5334ff0946 (MD5)Approved for entry into archive by Nuzia Santos ([email protected]) on 2019-07-31T16:57:32Z (GMT) No. of bitstreams: 2 cristiani_castilho.pdf: 1486413 bytes, checksum: c013a7bd4c83b4837986dd5334ff0946 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5)Made available in DSpace on 2019-07-31T16:57:32Z (GMT). No. of bitstreams: 2 cristiani_castilho.pdf: 1486413 bytes, checksum: c013a7bd4c83b4837986dd5334ff0946 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2011Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Belo Horizonte, MG, Brasil.A importância das leishmanioses reside na alta incidência, ampla distribuição geográfica, possibilidade de assumir formas graves, com altas taxas de mortalidade nos casos não tratados de leishmaniose visceral (LV) e alta morbidade nos casos de leishmaniose tegumentar (LT). A principal forma de transmissão do parasito ocorre pela picada de fêmeas de flebotomíneos infectadas e o cão é considerado o principal reservatório doméstico. O objetivo deste trabalho foi estudar aspectos epidemiológicos das leishmanioses relacionados à infecção canina e aos flebotomíneos no município de Várzea da Palma, MG. Foram realizadas coletas sistematizadas mensais para captura de flebotomíneos durante um ano, utilizando armadilhas luminosas HP e não sistematizadas, com capturas manuais utilizando capturador de Castro. As fêmeas coletadas foram identificadas e submetidas à verificação de infecção natural pela dissecção e detecção de DNA do parasito (LnPCR) em amostras específicas contendo até 20 fêmeas cada. A densidade total de flebotomíneos foi correlacionada com dados climatológicos. No estudo da infecção canina foi verificada a soroprevalência utilizando as técnicas sorológicas RIFI e ELISA. Ainda como método diagnóstico amostras clínicas de medula óssea, pele (de 41 cães sorologicamente positivos) e sangue periférico (207 cães) foram utilizadas na técnica da LnPCR. O isolamento e caracterização do parasito foram realizados utilizando amostras positivas da cultura de aspirado de medula óssea e amostras clínicas positivas dos 41 cães reativos na sorologia. Foram coletados 18.154 machos e 7.180 fêmeas de flebotomíneos, pertencentes a quinze espécies, sendo a mais prevalente Nyssomyia intermedia (52%) seguida por Lutzomyia longipalpis (41%), importantes vetoras de LT e LV respectivamente. Neste estudo foram identificados seis tipos de anomalias unilaterais e duas bilaterais, todas no parâmero de machos de Lu. longipalpis. Foi observada correlação estatisticamente significativa entre densidade total de flebotomíneos e a temperatura média durante o período de estudo. Nenhuma forma flagelada foi encontrada pela técnica da dissecção. Foi detectada a infecção natural nas seguintes espécies: Evandromyia evandroi (10,0%), Evandromyia lenti (5,0%), Evandromyia sallesi (2,8%), Evandromyia termitophila (16,7%), Lu. longipalpis (2,14%), Ny. intermedia (1,0%) todas infectadas com Leishmania chagasi. Ev. lenti e Lu. longipalpis apresentaram a taxa de infecção para Leishmania braziliensis de 5,0% e 0,6% respectivamente e Nyssomyia neivai, Psathyromyia shannoni e Micropygomyia quinquefer mostraram positividade para Leishmania sp. A soroprevalência canina foi de 24,6% sendo a maioria dos cães de raça indefinida e idade variando entre dois e cinco anos. Os testes sorológicos mostram-se mais eficazes em detectar os animais sintomáticos. Quanto às amostras clínicas, o sangue foi o menos eficiente em detectar a infecção no grupo dos 41 cães com sorologia positiva. A amostra de medula óssea foi a mais eficaz na detecção de animais positivos independentemente da sintomatologia e também foi capaz de identificar dentre o grupo de cães assintomáticos o maior número de animais positivos em relação as demais amostras biológicas utilizadas na LnPCR. Diante do encontro de cães infectados exclusivamente por Le. chagasi vivendo em áreas onde foram registrados casos humanos de LV e flebotomíneos infectados por este parasito fica demonstrado um ciclo ativo da Le. chagasi por todo o território urbano do município de Várzea da Palma, devendo as autoridades responsáveis manter uma constante realização de inquéritos caninos e vigilância entomológica para um melhor direcionamento e eficácia das medidas de controle.The importance of leishmaniasis is the high incidence, the wide geographical distribution and the ability to take severe forms with high mortality rates in untreated cases of visceral leishmaniasis (VL) and high morbidity in cases of cutaneous leishmaniasis (CL). The main form of parasite transmission occurs through the bite of infected sandflies females and the dog is the main domestic reservoir. This paper aimed to study epidemiological aspects of leishmaniasis related to canine infection and sandflies in the city of Várzea da Palma, Minas Gerais State. Systematic collections were monthly performed to capture sand flies over one year, using HP light traps, and unsystematic collections were performed as manual captures using Castro catcher. Collected females were identified and submitted to natural infection verification by dissection and detection of parasite’s DNA (LnPCR) in specific samples containing up to 20 females each. The total density of sandflies was correlated with climatological data. In the canine infection study, the seroprevalence was verified using the serological techniques IFA and ELISA. Even as a diagnostic method, clinical samples of bone marrow, skin (of 41 serologically positive dogs) and peripheral blood (207 dogs) were used in LnPCR technique. Isolation and characterization of parasite were performed using positive samples from the culture of aspirated bone marrow and positive clinical samples from 41 reactive dogs in serology. We collected 18.154 males and 7.180 females sand flies, belonging to 15 species of sandflies, which the most prevalent is Nyssomyia intermedia (52%) followed by Lutzomyia longipalpis (41%), both important vectors of CL and VL, respectively. This study identified 6 types of unilateral abnormalities and 2 types of bilateral, all of them in the paramere of Lu. longipalpis males. We observed statistically significant correlation between total density of sandflies and the average of temperature during the period of study. No flagellate form was found by the technique of dissection. Natural infection was detected in the following species: Evandromyia evandroi (10,0%), Evandromyia lenti (5,0%), Evandromyia sallesi (2.8%), Evandromyia termitophila (16.7%), Lu. longipalpis (2.1%), Ny. intermedia (1,0%), all infected by Leishmania chagasi. Ev. lenti and Lu. longipalpis showed infection rate for Leishmania braziliensis of 5,0% and 0.6%, respectively, and Nyssomyia neivai, Psathyromyia shannoni and Micropygomyia quinquefer showed positivity for Leishmania sp. Canine seroprevalence was 24.6% with most of them mongrel dogs and ages ranging from two to five years. Serological tests were more effective in detecting symptomatic animals. As for clinical samples, blood was the least effective in detecting infection in the group of 41 dogs with positive serology. The bone marrow sample was most effective in detecting positive animals regardless of symptoms and was also able to identify among the group of asymptomatic dogs the highest number of positive animals when compared with other biological samples used in LnPCR. Against the finding of infected dogs exclusively by Le. chagasi living in areas where human cases of VL were recorded and infected sandflies by this parasite, is demonstrated an active cycle of Le. chagasi in the entire urban territory of Várzea da Palma, about what the authorities should maintain a constant canine surveys and entomological surveillance for better targeting and effectiveness of control measures

Epidemiologia das Leishmanioses no Distrito de Barra do Guaicui, Município de Várzea da Palma, Minas Gerais, Brasil

Submitted by Nuzia Santos ([email protected]) on 2016-09-08T13:19:05Z No. of bitstreams: 1 Tese_DIP_CristianideCastilhoSanguinette - T_97.pdf: 18450256 bytes, checksum: 4cda2f86042db8ee71128cabc8d9436b (MD5)Approved for entry into archive by Nuzia Santos ([email protected]) on 2016-09-08T18:05:56Z (GMT) No. of bitstreams: 1 Tese_DIP_CristianideCastilhoSanguinette - T_97.pdf: 18450256 bytes, checksum: 4cda2f86042db8ee71128cabc8d9436b (MD5)Made available in DSpace on 2016-09-08T18:05:56Z (GMT). No. of bitstreams: 1 Tese_DIP_CristianideCastilhoSanguinette - T_97.pdf: 18450256 bytes, checksum: 4cda2f86042db8ee71128cabc8d9436b (MD5) Previous issue date: 2015Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.As leishmanioses são um complexo de doenças e sua epidemiologia somente pode ser compreendida pelo conhecimento de todos os elos que compõem seu ciclo de transmissão, como reservatórios, vetores e espécies de parasitos envolvidos e suas relações ecológicas. O objetivo deste trabalho foi estudar a epidemiologia das leishmanioses no distrito de Barra do Guaicui, município de Várzea da Palma, Minas Gerais. Foram realizadas no ano de 2012 cinco coletas de flebotomíneos em 24 casas da área urbana e doze coletas durante os anos de 2013 e 2014 em três ambientes distintos (urbano, transição e mata), utilizando armadilhas luminosas HP. As fêmeas coletadas foram identificadas e submetidas à verificação de infecção natural pela dissecção do tubo digestório e a detecção de DNA do parasito (LnPCR) em amostras individuais. Para o segundo período de estudo foram verificados e comparados os padrões de distribuição, a riqueza, uniformidade, e abundância dos flebotomíneos nas diferentes áreas. Para o estudo dos hospedeiros silvestres e sinantrópicos de Leishmania foram instaladas duas armadilhas, uma do tipo Sherman e outra do tipo gaiola, em cada uma das casas amostradas para o ano de 2012 e, além destes pontos foram definidas três trilhas, com 15 armadilhas tipo Sherman e 15 tipo gaiola por trilha, em área limítrofe ao perímetro urbano. No ano de 2013 as coletas de pequenos mamíferos foram conduzidasnos mesmos pontos de coleta dos flebotomíneos e utilizando a mesma metodologia. O estudo da infecção dos pequenos mamíferos foi realizado utilizando técnicas moleculares. Um total de 5.831 flebotomíneos pertencentes a quinze espécies e oito gêneros foi coletado e identificado. A espécie mais prevalente foi Nyssomyia intermedia, seguida de Lutzomyia longipalpis, importantes vetoras dos agentes etiológicos da forma tegumentar e visceral em humanos, respectivamente. A área urbana teve a maior abundância enquanto a área de transição teve a maior diversidade e uniformidade de espécies. Nyssomyia intermedia foi a espécie mais abundante na área urbana, enquanto Evandromyia evandroi foi a mais abundante na área de transição e Ev. lenti na área silvestre. Nenhuma forma flagelada foi encontrada pela técnica da dissecção. Foi detectada a presença de DNA de Leishmania nas seguintes espécies: Ny. intermedia (0,9%), Lu. longipalpis (2,9%), Ev. termitophila (3,0%), Ev. sallesi (1,8%), Ev. evandroi (1,5%), Ev. neivai (1,1%), Ev lenti (0,9%) e Ev. walkeri (9,0%) todas com DNA de parasitos do complexo Le. braziliensis. Nyssomyia intermedia (0,3%), Ev. evandroi (1,5%), Ev. lenti (1,8%), Ev. sallesi (1,2%), Lu. longipalpis (0,58%), Ny. neivai (1,1%) e Psathyromyia lutziana (33,3%) apresentaram positividade para parasitos do complexo Le. donovani. Das 47 amostras positivas para a presença de DNA de Leishmania, 34 (72,3%) eram provenientes da área urbana, três (6,4%) da área de transição e dez (21,3%) da área de mata. A fauna de pequenos mamíferos foi composta pelas espécies Rattus rattus (19,4%), Didelphis albiventris (44,4%) e Thricomys apereoides (36,1%). Rattus rattus foi capturado apenas na área urbana, T. apereoides apenas na área silvestre e D. albiventris nos três ambientes amostrados. As duas últimas espécies foram encontradas infectadas por Le. braziliensis na área de mata e na de transição, respectivamente. Nossos resultados contribuem para a compreensão do processo gradual de sinantropização das espécies de flebotomíneos encontradas no estado de Minas Gerais, demostrando que o município de Várzea da Palma apresenta todas as características necessárias para a expansão das leishmanioses, visceral e tegumentar.Leishmaniasis are a complex of diseases and their epidemiology is understood only if all the links that compound its transmission cycle are known, such as reservoirs, vectors and parasites species and their ecological relationships. The aim of this research was to study the epidemiology of leishmaniasis in the District of Barra do Guaicui, municipality of Varzea da Palma, Minas Gerais State, Brazil. Five collections of phlebotomines were performed in 2012 in 24 houses in the urban area and another twelve collections from 2013 to 2014 in three distinct environments (urban, transition and wild forest), utilizing HP light traps. The collected females were identified and submitted to natural infection assessment through dissection of the digestive tract and parasite DNA detection (LnPCR) in individual samples. For the second period of study it was verified and compared the distribution richness, uniformity and abundance of sand flies in the different areas. For the study of wild and synanthropic hosts of Leishmania two traps were installed in each house sampled during 2012 and three tracks in a boundary area of urban perimeter were set, with 15 Sherman traps and 15 “cage” traps per track. Collections of small mammals were performed in 2013, in the same collections points of sand flies and with the same methodology used in 2012. The detection of Leishmania infection in small mammals was carried out through molecular techniques. A total of 5.831 phlebotomines belonging to fifteen species and eight genera were collected and identified. The most frequent species was Nyssomyia intermedia followed by Lutzomyia longipalpis, which are important vectors of the etiologic agents of cutaneous and visceral leishmaniasis in humans, respectively. The urban area had the highest abundance while the transition area has the highest diversity and uniformity of species. Nyssomyia intermedia was the most abundant species of the urban area, while Evandromyia evandroi was the most abundant in the transition area and Ev. lenti in the wild area. No flagellate forms were found by the dissection technique. Leishmania DNA was detected in the following species: Ny. intermedia (0,9%), Lu. longipalpis (2,9%), Ev. termitophila (3,0%), Ev. sallesi (1,8%), Ev. evandroi (1,5%), Ev. neivai (1,1%), Ev lenti (0,9%) and Ev. walkeri (9,0%), all of them with DNA of Le. braziliensis complex. Nyssomyia intermedia (0,3%), Ev. evandroi (1,5%), Ev. lenti (1,8%), Ev. sallesi (1,2%), Lu. longipalpis (0,58%), Ny. neivai (1,1%) and Psathyromyia lutziana (33,3%) presented positivity for the Le. donovani complex. Out of 47 Leishmania positive samples, 34 (72,3%) were from the urban area, three (6,4%) from the transition area and ten (21,3%) from the wild area. The small mammals fauna was composed by the species Rattus rattus (19,4%), Thricomys apereoides (36,1%) and Didelphis albiventris (44,4%) . Rattus rattus was captured exclusively in urban area, T. apereoides only in the forest area and D. albiventris in the three environments. The last two animals were found infected by Le. braziliensis in the wild and transitional areas, respectively. Our results contributes to understand the gradual process of synanthropism of the sand flies species found in the state of Minas Gerais, demonstrating that the municipality of Varzea da Palma has all the necessary characteristics for the expansion of leishmaniasis, visceral and cutaneous

Description of Evandromyia (Aldamyia) orcyi; a new phlebotomine species (Diptera: Psychodidae: Phlebotominae) from the State of Mato Grosso do Sul; Brazil

Submitted by Nuzia Santos ([email protected]) on 2016-01-29T17:18:18Z No. of bitstreams: 1 Description of Evandromyia (Aldamyia) orcyi, a new phlebotomine species.pdf: 5088530 bytes, checksum: abf57195ffa4767806f9d63c8c6225e0 (MD5)Approved for entry into archive by Nuzia Santos ([email protected]) on 2016-01-29T17:20:55Z (GMT) No. of bitstreams: 1 Description of Evandromyia (Aldamyia) orcyi, a new phlebotomine species.pdf: 5088530 bytes, checksum: abf57195ffa4767806f9d63c8c6225e0 (MD5)Made available in DSpace on 2016-01-29T17:20:55Z (GMT). No. of bitstreams: 1 Description of Evandromyia (Aldamyia) orcyi, a new phlebotomine species.pdf: 5088530 bytes, checksum: abf57195ffa4767806f9d63c8c6225e0 (MD5) Previous issue date: 2015Universidade Federal de Mato Grosso do Sul. Centro de Ciências Biológicas e da Saúde. Laboratório de Parasitologia Humana. Campo Grande, MS, Brasil.Centro de Referência Nacional e Internacional para Flebotomíneos. Coleção de Flebotomíneos. Grupo de Estudos em Leishmanioses. Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Secretaria de Estado de Saúde. Núcleo Regional de Saúde. Laboratório Regional de Entomologia. Dourados, MS, Brasil.Centro de Referência Nacional e Internacional para Flebotomíneos. Coleção de Flebotomíneos. Grupo de Estudos em Leishmanioses. Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Background: The genus Evandromyia is widely found in Brazil, but occurs mainly in Brazilian savannah. To date 13 species have been described in the subgenus Aldamyia. Here we described a new species of Evandromyia (Aldamyia) collected in the State of Mato Grosso do Sul, Brazil. Methods: Measurements were made using a micrometer eyepiece on an Olympus CH-2 binocular microscope and drawings were executed with the aid of a camera lucida. Results: The new species, Evandromyia orcyi sp. nov., is closely related to Evandromyia lenti, Evandromyia carmelinoi and Evandromyia evandroi, however, characteristics of the male terminalia and female spermathecae distinguish it from other species of the genus Evandromyia. Conclusion: With the description of Evandromyia orcyi sp. nov., six species of the subgenus Aldamyia have been reported from the State of Mato Grosso do Sul

Description of a new phlebotomine species, Martinsmyia reginae sp. nov. (Diptera: Psychodidae: Phlebotominae) from a cave in the state of Tocantins Brazil

As inhabitants of forested areas, caves and anthropic environments, the phlebotomines deserve special attention because some species are able to transmit trypanosomatids, bacteria and viruses to vertebrates. Phlebotomines are also a nuisance because they cause painful bites, which may ultimately produce allergic manifestations. The lack of information about the presence and behaviours of sand flies in caves has aroused the curiosity of researchers for a long time. In the present paper, we describe a new species of sand fly that was captured in a cave located in the municipal district of Arraias in the southeastern region of the state of Tocantins. The morphological features of this new species permit it to be included in the alphabetica group of the Martinsmyia genus

Sandflies (Diptera: Psychodidae: Phlebotominae) collected on the banks of the Velhas River in the state of Minas Gerais, Brazil

Leishmaniases are endemic to several Brazilian states, including Minas Gerais. As many cases of these diseases are diagnosed in Minas Gerais every year, this paper aimed to determine the diversity of the species of sandflies vector of leishmaniases, in Lassance and Corinto on the banks of the Velhas River. Over two years, 21,014 specimens of sandflies, belonging to 19 species were captured with light traps. Of these specimens, Nyssomyia neivai (Pinto 1926) (84.18%), Nyssomyia intermedia (Lutz &amp; Neiva 1912) (13.41%), Evandromyia lenti(Mangabeira 1938) (0.99%) and Evandromyia sallesi (Galvão &amp; Coutinho 1939) (0.48%) were the most represented. The former two species could be involved in the epidemiological chain of leishmaniasis in this region. No significant difference was found between the species compositions on the two river banks

Hourly activity and natural infection of sandflies (Diptera: Psychodidae) captured from the aphotic zone of a cave, Minas Gerais State, Brazil

Submitted by Nuzia Santos ([email protected]) on 2014-03-07T15:53:38Z No. of bitstreams: 1 Hourly activity and natural infection of sandflies .pdf: 1399060 bytes, checksum: 53bdcfbfdb50a2f9f57973781c760d59 (MD5)Made available in DSpace on 2014-03-07T15:53:38Z (GMT). No. of bitstreams: 1 Hourly activity and natural infection of sandflies .pdf: 1399060 bytes, checksum: 53bdcfbfdb50a2f9f57973781c760d59 (MD5) Previous issue date: 2012Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Bioquímica de Insetos. Rio de Janeiro, RJ, BrazilSandflies are holometabolous insects that are of great epidemiological importance in the neotropical region as vectors of leishmaniases. Caves are ecotopes that significantly differ from external environments and, among the insects that live or visit their internal area and adjacent environment, sandflies are commonly found. Based on this context, the objective of this work was to examine the period of activity of sandflies in the cave environment in the aphotic zone. Thus, four sandfly captures were conducted, one in each season of the year, in a cave where studies on the bioecological aspects of sandfly fauna have been conducted since 2008. In this same study, we have also noticed the presence of flagellates in some captured females. Catches were carried out for 24 hours using a Shannon trap, light bait, and cave walls were actively searched. We collected a total of 638 sandflies, representing 11 species. The most abundant species and with more intense period of activity were, in descending order: Lu. cavernicola (62%), Ev. spelunca (16%) and Ev. sallesi (14%). A total of 69 females were dissected to check for natural infection, and in five specimens we found living flagellated forms: two Ev. spelunca, two Ev. sallesi and one Sc. sordellii. This study shows that the activity of some species caught in the aphotic zone of the cave, especially Lu. cavernicola, differs from what has already been reported in previous sandfly captures, which are almost always conducted at night and during twilight. The existence of sandflies that were naturally infected with flagellates and the lack of awareness regarding the behaviour of sandflies in cave environments are strong indicators of the need for further study on this group of insects in this ecotope, as a safety measure to protect the visitors of such environment

Comparison of the phlebotomine (Diptera: Psychodidae) fauna of urban, transitional, and wild areas in northern Minas Gerais, Brazil

Submitted by Nuzia Santos ([email protected]) on 2016-04-06T12:32:52Z No. of bitstreams: 1 Comparison of the phlebotomine .pdf: 8475626 bytes, checksum: 793e507786977ec9a9454b256e06ac31 (MD5)Approved for entry into archive by Nuzia Santos ([email protected]) on 2016-04-06T13:32:59Z (GMT) No. of bitstreams: 1 Comparison of the phlebotomine .pdf: 8475626 bytes, checksum: 793e507786977ec9a9454b256e06ac31 (MD5)Made available in DSpace on 2016-04-06T13:32:59Z (GMT). No. of bitstreams: 1 Comparison of the phlebotomine .pdf: 8475626 bytes, checksum: 793e507786977ec9a9454b256e06ac31 (MD5) Previous issue date: 2015Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Centro de Referencia Nacional e Internacional para Flebotomineos. Coleção de Flebotomineos. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Centro de Referencia Nacional e Internacional para Flebotomineos. Coleção de Flebotomineos. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Centro de Referencia Nacional e Internacional para Flebotomineos. Coleção de Flebotomineos. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Centro de Referencia Nacional e Internacional para Flebotomineos. Coleção de Flebotomineos. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Centro de Referencia Nacional e Internacional para Flebotomineos. Coleção de Flebotomineos. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Centro de Referencia Nacional e Internacional para Flebotomineos. Coleção de Flebotomineos. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Centro de Referencia Nacional e Internacional para Flebotomineos. Coleção de Flebotomineos. Belo Horizonte, MG, Brasil.Background: Phlebotomines are directly related to the study of leishmaniases, and so the study of their distribution plays an important role in the epidemiology of these diseases. Collections of phlebotomines were made with the intent of comparing the distribution, richness, diversity, and abundance of species in three distinct environments in an area endemic for tegumentary and visceral leishmaniasis in Minas Gerais State, Brazil. Methods: Phlebotomines were collected with automatic light traps in urban, transitional, and wild areas from March 2013 to February 2014 in the district of Barra do Guaicuí, municipality of Várzea da Palma, Minas Gerais. The distribution patterns of these species of insects, as well as species richness, evenness, and abundance among the different areas, were analyzed. Results: A total of 3,365 phlebotomines belonging to 15 species were collected. The urban area had the greatest abundance whereas the transitional area had the greatest diversity and evenness of species. Nyssomyia intermedia was the most abundant species in the urban area, whereas Evandromyia evandroi was the most abundant in the transitional area and Ev. lenti in the wild area. Conclusion: The analysis of our results showed that the distribution of the collected species had distinct profiles between the environments studied. Furthermore our study indicates the potential risk of transmission of leishmaniasis in the urban environment where it was observed had the highest population density and abundance of important vector species of Leishmania