Análisis del Ciclo de Vida de una Unidad Prototipo de Vivienda de Emergencia. La búsqueda del impacto nulo

Prototype Unit of Emergency Housing (PUEH) is the result of the Applied Research Project VEM (Military Emergency Housing) developed in collaboration with Escuela Politécnica Superior (Universidad CEU) and the company Air-bus Defense & Space. It is designed as a modular and industrialized unit of basic habitability, with programmed and expandable growth, designed to provide shelter and protection in environments of humanitarian crises or contingencies of social vulnerability in order to ensure sustainable habitat for emergencies.The influence of the construction processes and materials involved in the manufacture of this PUEH have on the environment, analyzed using the methodology of life-cycle assessment (LCA), considered especially critical recycling the mate-rials used. Thus, in order to reduce the environmental impact environmental, each of the component parts of the developed prototype unit are quantified, evaluating the benefits resulting from the methodology DfMA (Design for Manufacturing and Assembly).La Unidad Prototipo de Vivienda de Emergencia (UPVE) es el resultado del Proyecto de Investigación Aplicada VEM (Vivienda de Emergencia Militar) desarrollado con la colaboración de la Escuela Politécnica Superior de la Universidad CEU y la empresa Airbus Defense & Space. Es concebido como una unidad modular e industrializable de habitabilidad básica, de crecimiento programado y extensible, diseñada para proporcionar refugio y protección en entornos de crisis humanitaria o en contingencias de vulnerabilidad social con el fin de asegurar un hábitat sostenible de emergencia.La influencia que los procesos de construcción y materiales involucrados en la fabricación de dicha UPVE tienen en el medio ambiente, analizados mediante la metodología de Análisis de Ciclo de Vida (ACV), consideran especialmente crítico el reciclado de los materiales utilizados. Así, con el objetivo de analizar y disminuir el impacto medioambiental, se cuantifican cada una de las partes que componen la Unidad Prototipo desarrollada, valorando los beneficios resultantes de los procesos de fabricación en la disposición final de los materiales según la metodología DfMA (Design for Manufacturing and Assembly)

Socioeconomic differences in caesarean section - Are they explained by medical need? An analysis of patient record data of a large Kenyan hospital

Background: Caesarean section (C-section) rates are often low among the poor and very high among the better-off in low- and middle-income countries. We examined to what extent these differences are explained by medical need in an African context. Methods: We analyzed electronic records of 12,209 women who gave birth in a teaching hospital in Kenya in 2014. C-section rates were calculated by socioeconomic position (SEP), using maternal occupation (professional, small business, housewife, student) as indicator. We assessed if women had documented clinical indications according to hospital guidelines and if socioeconomic differences in C-section rates were explained by indication. Results: Indication for C-section according to hospital guidelines was more prevalent among professionals than housewives (16% vs. 9% of all births). The C-section rate was also higher among professionals than housewives (21.1% vs. 15.8% [OR 1.43; 95%CI 1.23-1.65]). This C-section rate difference was largely explained by indication (4.7 of the 5.3 percentage point difference between professionals and housewives concerned indicated C-sections, often with previous C-section as indication). Repeat C-sections were near-universal (99%). 43% of primary C-sections had no documented indication. Over-use was somewhat higher among professionals than housewives (C-section rate among women without indication: 6.6 and 5.5% respectively), which partly explained socioeconomic differences in primary C-section rate. Conclusions: Socioeconomic differences in C-section rates can be largely explained by unnecessary primary C-sections and higher supposed need due to previous C-section. Prevention of unnecessary primary C-sections and promoting safe trial of labor should be priorities in addressing C-section over-use and reducing inequalities. Tweetable abstract: Unnecessary primary C-sections and ubiquitous repeat C-sections drive overall C-section rates and C-section inequalities

The Cyclophilin-Binding Agent Sanglifehrin A Is a Dendritic Cell Chemokine and Migration Inhibitor

Sanglifehrin A (SFA) is a cyclophilin-binding immunosuppressant but the immunobiology of action is poorly understood. We and others have reported that SFA inhibits IL-12 production and antigen uptake in dendritic cells (DC) and exhibits lower activity against lymphocytes. Here we show that SFA suppresses DC chemokine production and migration. Gene expression analysis and subsequent protein level confirmation revealed that SFA suppressed CCL5, CCL17, CCL19, CXCL9 and CXCL10 expression in human monocyte-derived DC (moDC). A systems biology analysis, Onto Express, confirmed that SFA interferes with chemokine-chemokine receptor gene expression with the highest impact. Direct comparison with the related agent cyclosporine A (CsA) and dexamethasone indicated that SFA uniquely suppresses moDC chemokine expression. Competitive experiments with a 100-fold molar excess of CsA and with N-Methyl-Val-4-cyclosporin, representing a nonimmunosuppressive derivative of CsA indicated chemokine suppression through a cyclophilin-A independent pathway. Functional assays confirmed reduced migration of CD4+ Tcells and moDCs to supernatant of SFA-exposed moDCs. Vice versa, SFA-exposed moDC exhibited reduced migration against CCL19. Moreover, SFA suppressed expression of the ectoenzyme CD38 that was reported to regulate DC migration and cytokine production. These results identify SFA as a DC chemokine and migration inhibitor and provide novel insight into the immunobiology of SFA

Methods of probing the interactions between small molecules and disordered proteins

It is generally recognized that a large fraction of the human proteome is made up of proteins that remain disordered in their native states. Despite the fact that such proteins play key biological roles and are involved in many major human diseases, they still represent challenging targets for drug discovery. A major bottleneck for the identification of compounds capable of interacting with these proteins and modulating their disease-promoting behaviour is the development of effective techniques to probe such interactions. The difficulties in carrying out binding measurements have resulted in a poor understanding of the mechanisms underlying these interactions. In order to facilitate further methodological advances, here we review the most commonly used techniques to probe three types of interactions involving small molecules: (1) those that disrupt functional interactions between disordered proteins; (2) those that inhibit the aberrant aggregation of disordered proteins, and (3) those that lead to binding disordered proteins in their monomeric states. In discussing these techniques, we also point out directions for future developments.Gabriella T. Heller is supported by the Gates Cambridge Trust Scholarship. Francesco A. Aprile is supported by a Senior Research Fellowship award from the Alzheimer’s Society, UK (grant number 317, AS-SF-16-003)