2 research outputs found
Optimization of Media Change Intervals through Hydrogels Using Mathematical Models
Three-dimensional cell culture in engineered hydrogels
is increasingly
used in tissue engineering and regenerative medicine. The transfer
of nutrients, gases, and waste materials through these hydrogels is
of utmost importance for cell viability and response, yet the translation
of diffusion coefficients into practical guidelines is not well established.
Here, we combined mathematical modeling, fluorescent recovery after
photobleaching, and hydrogel diffusion experiments on cell culture
inserts to provide a multiscale practical approach for diffusion.
We observed a dampening effect of the hydrogel that slowed the response
to concentration changes and the creation of a diffusion gradient
in the hydrogel by media refreshment. Our designed model combined
with measurements provides a practical point of reference for diffusion
coefficients in real-world culture conditions, enabling more informed
choices on hydrogel culture conditions. This model can be improved
in the future to simulate more complicated intrinsic hydrogel properties
and study the effects of secondary interactions on the diffusion of
analytes through the hydrogel
Optimization of Media Change Intervals through Hydrogels Using Mathematical Models
Three-dimensional cell culture in engineered hydrogels
is increasingly
used in tissue engineering and regenerative medicine. The transfer
of nutrients, gases, and waste materials through these hydrogels is
of utmost importance for cell viability and response, yet the translation
of diffusion coefficients into practical guidelines is not well established.
Here, we combined mathematical modeling, fluorescent recovery after
photobleaching, and hydrogel diffusion experiments on cell culture
inserts to provide a multiscale practical approach for diffusion.
We observed a dampening effect of the hydrogel that slowed the response
to concentration changes and the creation of a diffusion gradient
in the hydrogel by media refreshment. Our designed model combined
with measurements provides a practical point of reference for diffusion
coefficients in real-world culture conditions, enabling more informed
choices on hydrogel culture conditions. This model can be improved
in the future to simulate more complicated intrinsic hydrogel properties
and study the effects of secondary interactions on the diffusion of
analytes through the hydrogel