Certification of damage tolerant composite structure

A reliability based certification testing methodology for impact damage tolerant composite structure was developed. Cocured, adhesively bonded, and impact damaged composite static strength and fatigue life data were statistically analyzed to determine the influence of test parameters on the data scatter. The impact damage resistance and damage tolerance of various structural configurations were characterized through the analysis of an industry wide database of impact test results. Realistic impact damage certification requirements were proposed based on actual fleet aircraft data. The capabilities of available impact damage analysis methods were determined through correlation with experimental data. Probabilistic methods were developed to estimate the reliability of impact damaged composite structures

Out of plane analysis for composite structures

Simple two dimensional analysis techniques were developed to aid in the design of strong joints for integrally stiffened/bonded composite structures subjected to out of plane loads. It was found that most out of plane failures were due to induced stresses arising from rapid changes in load path direction or geometry, induced stresses due to changes in geometry caused by buckling, or direct stresses produced by fuel pressure or bearing loads. While the analysis techniques were developed to address a great variety of out of plane loading conditions, they were primarily derived to address the conditions described above. The methods were developed and verified using existing element test data. The methods were demonstrated using the data from a test failure of a high strain wingbox that was designed, built, and tested under a previous program. Subsequently, a set of design guidelines were assembled to assist in the design of safe, strong integral composite structures using the analysis techniques developed

Proceedings of the Third Caldwell Conference, St. Catherines Island, Georgia, May 9-11, 2008

341 p. : ill. (some col.), maps (some col.) ; 26 cm. "Issued June 23, 2010." Includes bibliographical references (p. 303-341).The late Archaic of the American Southeast is typically described as a time of population growth, innovative developments in subsistence strategies, and increased social complexity. Although it is difficult to generalize, many early Woodland communities are characterized as relatively small scale, fairly mobile foragers organized into unranked or minimally ranked lineages and clans. Early Woodland groups also seem to be more socially isolated than their late Archaic predecessors, with a decline in regional exchange networks. The papers in this volume were presented at a conference entitled "What Happened in the Late Archaic?" which was co-sponsored by the American Museum of Natural History and the St. Catherines Island Foundation and held on St. Catherines Island (Georgia), May 9-11, 2008. The Third Caldwell Conference invited the participants to engage the appropriate archaeological data from the American Southeast, specifically addressing the nature of change during the late Archaic-early Woodland transition. This volume consists of a dozen substantive papers, followed by three discussant contributions. TABLE OF CONTENTS: Trend, tradition, and transition at the end of the Archaic / Tristram R. Kidder -- "Nothing but the river's flood" : late Archaic diaspora or disengagement in the lower Mississippi Valley and southeastern North America / Jon L. Gibson -- The two rings of St. Catherines Island : some preliminary results from the St. Catherines and McQueen shell rings / Matthew C. Sanger and David Hurst Thomas -- Two late Archaic period shell rings, St. Simon's Island, Georgia / Rochelle A. Marrinan -- The Archaic above Choctawhatchee Bay : hydrodynamics, adaptation, and abandonment / Rebecca Saunders -- Prehistoric landscapes of complexity : Archaic and Woodland period shell works, shell rings, and tree islands of the Everglades, South Florida / Margo Schwadron -- Shell rings and other settlement features as indicators of cultural continuity between the late Archaic and Woodland periods of coastal Florida / Michael Russo -- "What happened to the southeastern Archaic?" : a perspective from St. Catherines Island / David Hurst Thomas -- Leaving the rings : shell ring abandonment and the end of the late Archaic / Matthew C. Sanger -- The rhythms of space-time and the making of monuments and places during the Archaic / Victor D. Thompson -- Getting from the late Archaic to early Woodland in three middle valleys (those being the Savannah, St. Johns, and Tennessee) / Kenneth E. Sassaman -- Late Archaic? : what the hell happened to the middle Archaic? / Joe Saunders -- Thoughts on the late Archaic-early Woodland transition on the Georgia and South Carolina coasts / Chester B. DePratter -- Mounds, middens, and rapid climate change during the Archaic-Woodland transition in the southeastern United States / William H. Marquardt -- The end of the southeastern Archaic : regional interaction and archaeological interpretation / David G. Anderson

Genome-Scale Reconstruction and Analysis of the Pseudomonas putida KT2440 Metabolic Network Facilitates Applications in Biotechnology

A cornerstone of biotechnology is the use of microorganisms for the efficient production of chemicals and the elimination of harmful waste. Pseudomonas putida is an archetype of such microbes due to its metabolic versatility, stress resistance, amenability to genetic modifications, and vast potential for environmental and industrial applications. To address both the elucidation of the metabolic wiring in P. putida and its uses in biocatalysis, in particular for the production of non-growth-related biochemicals, we developed and present here a genome-scale constraint-based model of the metabolism of P. putida KT2440. Network reconstruction and flux balance analysis (FBA) enabled definition of the structure of the metabolic network, identification of knowledge gaps, and pin-pointing of essential metabolic functions, facilitating thereby the refinement of gene annotations. FBA and flux variability analysis were used to analyze the properties, potential, and limits of the model. These analyses allowed identification, under various conditions, of key features of metabolism such as growth yield, resource distribution, network robustness, and gene essentiality. The model was validated with data from continuous cell cultures, high-throughput phenotyping data, 13C-measurement of internal flux distributions, and specifically generated knock-out mutants. Auxotrophy was correctly predicted in 75% of the cases. These systematic analyses revealed that the metabolic network structure is the main factor determining the accuracy of predictions, whereas biomass composition has negligible influence. Finally, we drew on the model to devise metabolic engineering strategies to improve production of polyhydroxyalkanoates, a class of biotechnologically useful compounds whose synthesis is not coupled to cell survival. The solidly validated model yields valuable insights into genotype–phenotype relationships and provides a sound framework to explore this versatile bacterium and to capitalize on its vast biotechnological potential

The Concise Guide to PHARMACOLOGY 2023/24: G protein-coupled receptors.

peer reviewedThe Concise Guide to PHARMACOLOGY 2023/24 is the sixth in this series of biennial publications. The Concise Guide provides concise overviews, mostly in tabular format, of the key properties of approximately 1800 drug targets, and about 6000 interactions with about 3900 ligands. There is an emphasis on selective pharmacology (where available), plus links to the open access knowledgebase source of drug targets and their ligands (https://www.guidetopharmacology.org), which provides more detailed views of target and ligand properties. Although the Concise Guide constitutes almost 500 pages, the material presented is substantially reduced compared to information and links presented on the website. It provides a permanent, citable, point-in-time record that will survive database updates. The full contents of this section can be found at http://onlinelibrary.wiley.com/doi/bph.16177. G protein-coupled receptors are one of the six major pharmacological targets into which the Guide is divided, with the others being: ion channels, nuclear hormone receptors, catalytic receptors, enzymes and transporters. These are presented with nomenclature guidance and summary information on the best available pharmacological tools, alongside key references and suggestions for further reading. The landscape format of the Concise Guide is designed to facilitate comparison of related targets from material contemporary to mid-2023, and supersedes data presented in the 2021/22, 2019/20, 2017/18, 2015/16 and 2013/14 Concise Guides and previous Guides to Receptors and Channels. It is produced in close conjunction with the Nomenclature and Standards Committee of the International Union of Basic and Clinical Pharmacology (NC-IUPHAR), therefore, providing official IUPHAR classification and nomenclature for human drug targets, where appropriate

THE CONCISE GUIDE TO PHARMACOLOGY 2021/22: G protein-coupled receptors.

The Concise Guide to PHARMACOLOGY 2021/22 is the fifth in this series of biennial publications. The Concise Guide provides concise overviews, mostly in tabular format, of the key properties of nearly 1900 human drug targets with an emphasis on selective pharmacology (where available), plus links to the open access knowledgebase source of drug targets and their ligands (www.guidetopharmacology.org), which provides more detailed views of target and ligand properties. Although the Concise Guide constitutes over 500 pages, the material presented is substantially reduced compared to information and links presented on the website. It provides a permanent, citable, point-in-time record that will survive database updates. The full contents of this section can be found at http://onlinelibrary.wiley.com/doi/bph.15538. G protein-coupled receptors are one of the six major pharmacological targets into which the Guide is divided, with the others being: ion channels, nuclear hormone receptors, catalytic receptors, enzymes and transporters. These are presented with nomenclature guidance and summary information on the best available pharmacological tools, alongside key references and suggestions for further reading. The landscape format of the Concise Guide is designed to facilitate comparison of related targets from material contemporary to mid-2021, and supersedes data presented in the 2019/20, 2017/18, 2015/16 and 2013/14 Concise Guides and previous Guides to Receptors and Channels. It is produced in close conjunction with the Nomenclature and Standards Committee of the International Union of Basic and Clinical Pharmacology (NC-IUPHAR), therefore, providing official IUPHAR classification and nomenclature for human drug targets, where appropriate