Calcein release profile of the liposome with the formulations of varied phospholipid compositions.

<p>Phospholipid (DPPC/DSPC = 100-X/X):DSPE-PEG:cholesterol:SA-V6 = 55∶2∶10∶0.55 (X = 0, 25, 50, 75, and 100). Samples were measured after 5 min incubation at desired temperatures from 25 to 55°C by fluorometry at Ex. 493 nm/Em. 513 nm. Data is shown as mean ± S.D. (n = 3).</p

List of all simulations.

<p>List of all simulations.</p

DOX accumulation in the tumor at 1, 6, and 12 hr after i.v. injection combined with preheating.

<p>*, <i>p</i><0.005, #, <i>p</i><0.001, significant difference compared to e-TSL without preheating and free DOX with preheating.</p

DOX release profile of the liposome with the formulations.

<p>DPPC:DSPE-PEG:cholesterol = 55∶2∶15 in the presence/absence of SA-V3 (molar ratio∶0.41). The amounts of DOX release were measured after 5 min incubation at desired temperature from 25 to 55°C by fluorometry at Ex. 490 nm/Em. 615 nm. Data is shown as mean ± S.D. (n = 3).</p

Schematic diagram of e-TSL and the chemical structure of ELP-lipid conjugates.

<p>Schematic diagram of e-TSL and the chemical structure of ELP-lipid conjugates.</p

The protocol of the drug accumulation study with e-TSL-DOX and free DOX, administered under mild hyperthermia.

<p>The protocol of the drug accumulation study with e-TSL-DOX and free DOX, administered under mild hyperthermia.</p

Cryo-TEM images with varying amounts of DOX in the liposome.

<p>DPPC:DSPE-PEG:cholesterol:SA-V3 = 55∶2∶15∶0.41, with (A) liposome only, (B) 300 µg DOX/mL and (C) 500 µg DOX/mL.</p

Cryo-TEM images of the liposome at (A) 25°C, (B) 37°C, and (C) 42°C.

<p>Liposomes were composed of the formulation DPPC:DSPE-PEG:cholesterol:SA-V3 = 55∶2∶15∶0.41 and heat-treated for 5 min at indicated temperature in advance and followed by general cryo-TEM measurement.</p

List of ELP-lipid conjugates.

<p>List of ELP-lipid conjugates.</p

Average lateral diffusion coefficients (<i>D</i>) of DPPC lipids.

<p><i>D</i> was calculated from the slopes of the mean-square displacements in the xy-plane (the direction perpendicular to the bilayer normal), for the simulation systems with different ELP lengths (SA-V1, -V2, and V3) as functions of the molar ratio of (A) ELP and (B) cholesterol.</p