Nicotiana benthamiana protein, NbPCIP1, interacting with Potato virus X coat protein plays a role as susceptible factor for viral infection

AbstractThe interactions of viral coat protein (CP) and host factors play an important role in viral replication and/or host defense mechanism. In this study, we constructed Nicotiana benthamiana cDNA library to find host factors interacting with Potato virus X (PVX) CP. Using yeast two-hybrid assay, we screened 3.3×106 independent yeast transformants from N. benthamiana cDNA library and identified six positive clones. One positive clone, named PVX CP-interacting protein 1 (NbPCIP1), is a plant-specific protein with homologue in N. tabacum (GenBank accession no. AB04049). We confirmed the PVX CP–NbPCIP1 interaction using yeast-two hybrid assay in yeast, protein–protein binding assay in vitro, and bimolecular fluorescent complementation assay in planta. Quantitative real-time RT-PCR analysis showed that the mRNA level of NbPCIP1 increased in PVX-infected N. benthamiana plants as compared to that of healthy plants. The green fluorescent protein (sGFP)-fused NbPCIP1 (NbPCIP1-sGFP) was localized in ER or ER-associated granular-like structure of cells. When we co-express NbPCIP1-sGFP and red fluorescent protein (RFP)-fused PVX CP (PVX CP-RFP), which were introduced by transiently expressing these proteins in N. benthamiana protoplasts and epidermal cells, however, we observed the co-localization of these proteins in the inclusion body-like complex in areas surrounding nucleus. Transient over-expression and transgene silencing of NbPCIP1 assay analysis indicated that NbPCIP1 plays a critical role in viral replication during PVX infection in host plant

An Evaluation of Relative Damage to the Powertrain System in Tracked Vehicles

The objective of this study was to improve the reliability of the endurance test for the powertrain system of military tracked vehicles. The measurement system that measures the driving duty applied to the powertrain system caused by mobility on roads consists of eight analog channels and two pulse channels, including the propeller shaft output torques for the left and right sides. The data obtained from this measurement system can be used to introduce a new technology that produces the output torque of a torque converter and that can be applied to analyze the revolution counting for the endurance and road mobility in the front unit and represent the relative fatigue damages analysis technique and its results according to the driven roads through a cumulative fatigue method

Endogenous Private Leadership under Subsidy Policy on the Social Enterprises

We investigate a mixed oligopoly model in which private enterprises compete with social enterprises under government subsidy policy, and examine the endogenous choice of private leadership. We show that private leadership is socially desirable, but the numbers of private and social enterprises affect endogenous choices and welfare consequences. We also show that the role of government in choosing the optimal subsidy will be significant when there are more than one private enterprises but its number is smaller than that of the social enterprises

Subsidization Policy on the Social Enterprise for the Underprivileged

We formulate an oligopoly model in which social enterprise for the underprivileged competes with private enterprises under government subsidization, and examine the market role of private leadership. We show that Stackelberg private leadership is better from the viewpoint of total social welfare, while Cournot followership is better when the social provisions for the underprivileged are emphasized. We also find that both cost inefficiency and the number of private enterprises affect the profitability and welfare consequences. We then investigate the rationing policy on the production of social enterprise and show that output rationing is superior to market share rationing not only for the social concerns of the underprivileged but also for total social welfare, even though it is less attractive than subsidy policy. Finally, we find that there is a strategic over-incentive to pursue social activities under government subsidization

Endogenous Private Leadership under Subsidy Policy on the Social Enterprises

We investigate a mixed oligopoly model in which private enterprises compete with social enterprises under government subsidy policy, and examine the endogenous choice of private leadership. We show that private leadership is socially desirable, but the numbers of private and social enterprises affect endogenous choices and welfare consequences. We also show that the role of government in choosing the optimal subsidy will be significant when there are more than one private enterprises but its number is smaller than that of the social enterprises

Vertically aligned InGaN nanowires with engineered axial In composition for highly efficient visible light emission.

We report on the fabrication of novel InGaN nanowires (NWs) with improved crystalline quality and high radiative efficiency for applications as nanoscale visible light emitters. Pristine InGaN NWs grown under a uniform In/Ga molar flow ratio (UIF) exhibited multi-peak white-like emission and a high density of dislocation-like defects. A phase separation and broad emission with non-uniform luminescent clusters were also observed for a single UIF NW investigated by spatially resolved cathodoluminescence. Hence, we proposed a simple approach based on engineering the axial In content by increasing the In/Ga molar flow ratio at the end of NW growth. This new approach yielded samples with a high luminescence intensity, a narrow emission spectrum, and enhanced crystalline quality. Using time-resolved photoluminescence spectroscopy, the UIF NWs exhibited a long radiative recombination time (τr) and low internal quantum efficiency (IQE) due to strong exciton localization and carrier trapping in defect states. In contrast, NWs with engineered In content demonstrated three times higher IQE and a much shorter τr due to mitigated In fluctuation and improved crystal quality

Asynchronous Power Management Scheme for Wireless Ad-hoc Networks

Abstract-In wireless ad-hoc networks, power-saving of the terminals is very important issue since they operate depending on limited battery-power. To reduce the power consumption of the terminals, the terminals are allowed to enter into the LowPower Mode(LPM) in many wireless networks. However, owing to no clock synchronization of information transmission in adhoc networks, it occurs the problem that the terminals don't know when the neighbor terminal in the LPM becomes awake. In our paper, we proposed the novel power management scheme using the SLEEP TABLE to solve this problem. The simulation results show that the proposed scheme outperforms 802.ll LPM and the conventional scheme in terms of power saving, and the power consumption of each terminal is distributed