Evaluation of protective effect of cyclodextrin glucanotransferase-treated Gastrodia elata Blume extract on ultraviolet B-induced premature skin aging

Purpose: To investigate the protective effect of Gastrodia elata Blume (G. elata, GE) and cyclodextrin glucanotransferase (CGTase) enzyme-treated G. elata extract (EGE) against premature skin aging using ultraviolet B (UVB)-exposed normal human dermal fibroblasts (NHDFs).Methods: The extract was characterized by liquid chromatography with tandem mass spectrometry (LC-MS/MS), ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC–QToF–MS) and nuclear magnetic resonance spectroscopy (NMR). The expression of matrix  metalloproteinases (MMP-1,3), interleukin-6 (IL-6), transforming growth factor (TGF-β1) and procollagen type I was assayed using ELISA kits. Safety evaluation of EGE’s dietary administration and topical application was performed by in vivo acute oral toxicity and local lymph node tests.Results: Lower MMP-1 and IL-6 and higher procollagen type I and TGF-β1 levels were observed after treatment with EGE than with GE, indicating that EGE was more effective than GE in treating UVBinduced photoaging. With respect to phenolic composition, EGE had lower 4-hydroxybenzaldehyde (4- HBA) level and higher α-gastrodin level than GE. In UVB-irradiated NHDFs, α-gastrodin exhibited higher anti-aging activity than 4-HBA and β-gastrodin based on the expression of MMP-1, MMP-3, and procollagen type I. The in vivo data indicate that EGE was safe at concentrations of up to 2000 mg/kg for dietary administration and 0.1 % for topical application.Conclusion: EGE protects UVB-induced photoaged human skin better than GE owing to its higher α- gastrodin content. Thus, EGE may be potentially useful agent in anti-aging cosmetic products.Keywords: Gastrodia elata, α-Gastrodin, Anti-aging, CGTase, Ultraviolet B (UVB) irradiation, Matrix metalloproteinase, Procollagen, Normal human dermal fibroblast

Comparison between Matched Related and Alternative Donors of Allogeneic Hematopoietic Stem Cells Transplanted into Adult Patients with Acquired Aplastic Anemia: Multivariate and Propensity Score-Matched Analysis

We retrospectively compared the outcomes of 225 patients with adult acquired aplastic anemia (AA) who underwent allogeneic hematopoietic stem cell transplantation (alloHSCT) from matched related donors (MRDs), and those treated by alloHSCT from alternative donors (ADs). Univariate and multivariate analyses of factors associated with survival were performed. Multivariate analysis showed that age at alloHSCT of ≤31 years, MRD, successful engraftment, absence of acute graft-versus-host disease (aGVHD), and platelet engraftment at ≤21 days, were independent predictors of longer survival. In addition, time to aGVHD and cumulative nonrelapse mortality (NRM) were better in MRD than in AD recipients. Using propensity score matching (PSM), we performed a case-control study comparing 25 patients in each group who underwent alloHSCT from MRDs and ADs. Pretransplantation clinical factors were well balanced in either group. Median survival time was similar, and no statistically significant difference in transplantation outcomes was apparent when MRD and AD recipients were compared. In conclusion, our results suggest that alloHSCT from an AD should be considered earlier in adult patients with AA who do not have an MRD

Dynamic relocalization of NHERF1 mediates chemotactic migration of ovarian cancer cells toward lysophosphatidic acid stimulation

NHERF1/EBP50 (Na+/H+ exchanger regulating factor 1; Ezrin-binding phosphoprotein of 50 kDa) organizes stable protein complexes beneath the apical membrane of polar epithelial cells. By contrast, in cancer cells without any fixed polarity, NHERF1 often localizes in the cytoplasm. The regulation of cytoplasmic NHERF1 and its role in cancer progression remain unclear. In this study, we found that, upon lysophosphatidic acid (LPA) stimulation, cytoplasmic NHERF1 rapidly translocated to the plasma membrane, and subsequently to cortical protrusion structures, of ovarian cancer cells. This movement depended on direct binding of NHERF1 to C-terminally phosphorylated ERM proteins (cpERMs). Moreover, NHERF1 depletion downregulated cpERMs and further impaired cpERM-dependent remodeling of the cell cortex, suggesting reciprocal regulation between these proteins. The LPA-induced protein complex was highly enriched in migratory pseudopodia, whose formation was impaired by overexpression of NHERF1 truncation mutants. Consistent with this, NHERF1 depletion in various types of cancer cells abolished chemotactic cell migration toward a LPA gradient. Taken together, our findings suggest that the high dynamics of cytosolic NHERF1 provide cancer cells with a means of controlling chemotactic migration. This capacity is likely to be essential for ovarian cancer progression in tumor microenvironments containing LPA

Loan relation with foreign banks and information asymmetry: evidence from earnings management by local firms in Korea

Previous research shows that foreign (domestic) banks rely more on ‘hard’ (‘soft’) information in their lending decisions and such approaches may give a motivation for the local firms to improve their credit scores by, for example, overstating earnings. The paper develops a theoretical model and empirically tests the model using actual data from Korea. The model predicts that local firms tend to overstate earnings when they increase the loan relations with foreign banks and the proportion of local firms with earnings management increases as the recoverability of foreign banks decreases (increases) in the higher (lower) level of recoverability. The results of the empirical test are consistent with the predictions by the model. We also discuss the implications of the study and an extension for the future study

Evaluating blockchain technology adoption in multi-tier supply chains from an institutional entrepreneurship theory perspective

The presence of Blockchain Technology (BCT) and its potential supply chain (SC) and logistic benefits—such as improved resilience or lessened disruption—have motivated organizations to consider BCT investment. BCT is a relatively novel technology and requires multiple participants to meet its greatest potential. Its novelty is a barrier to its adoption and can be dependent on an innovator or leader to convince others to invest in or adopt BCT. Using an institutional entrepreneurship theoretical lens, we determine who should lead the investment (adoption) or whether a collaborative investment is best. Insights into this context can be garnered using a sequential game-theoretic model where lead investment roles switch amongst parties. Four scenarios are evaluated and consider full, partial, or no collaboration between SC entities. Within this framework, the two-stage process of adopting and implementing BCT is introduced to separate cost and demand channels. During the adoption stage, a reduction in marginal production cost via positive net benefits of BCT-based transportation and logistics is considered in the cost channel. In the implementation stage, the optimal level of BCT investment is determined, increasing shipping quality for consumers in transportation and logistics and reducing supply chain disruption simultaneously, which is considered in the demand channel. The results show that greater disruption likelihood will boost retail prices, intermediate prices, and profits of SC entities after BCT is adopted. We also find that traditional SCs with higher disruption levels result in lower BCT investment. When a leader in the sequential game (the manufacturer in our model) is the institutional entrepreneur, collaboration in BCT investment becomes more attractive. One important—and counterintuitive—result reveals that when full SC cooperation doesn\u27t exist, the entire SC is better off when the follower (not the leader) in a sequential game leads BCT adoption. The unique role of reduction in transportation and logistics costs via the cost channel is to compensate for a lower BCT investment due to lack of competition or reinforce a higher BCT investment by counterbalancing opportunistic behaviors. © 2024 Elsevier Lt