Urinary catechol amine levels, gastric secretion and specific psychological factors in ulcer and non-ulcer patients

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/32382/1/0000457.pd

Visualization and Analysis of mRNA Molecules Using Fluorescence <em>In Situ</em> Hybridization in <em>Saccharomyces cerevisiae</em>

The Fluorescence in situ Hybridization (FISH) method allows one to detect nucleic acids in the native cellular environment. Here we provide a protocol for using FISH to quantify the number of mRNAs in single yeast cells. Cells can be grown in any condition of interest and then fixed and made permeable. Subsequently, multiple single-stranded deoxyoligonucleotides conjugated to fluorescent dyes are used to label and visualize mRNAs. Diffraction-limited fluorescence from single mRNA molecules is quantified using a spot-detection algorithm to identify and count the number of mRNAs per cell. While the more standard quantification methods of northern blots, RT-PCR and gene expression microarrays provide information on average mRNAs in the bulk population, FISH facilitates both the counting and localization of these mRNAs in single cells at single-molecule resolution

Common and divergent features of galactose-1-phosphate and fructose-1-phosphate toxicity in yeast.

Metabolic dysregulation leading to sugar-phosphate accumulation is toxic in organisms ranging from bacteria to humans. By comparing two models of sugar-phosphate toxicity in Saccharomyces cerevisiae, we demonstrate that toxicity occurs, at least in part, through multiple, isomer-specific mechanisms, rather than a single general mechanism