Weevil_COI_data_Arlequin

The mitochondrial COI data of the camellia weevil in the Arlequin format

Camellia_SSR_data_GenAlEx

The microsatellite data of the Japanese camellia in the GenAlEx format

Expression profiles of DE sRNAs.

<p>Rows show sRNAs, and columns show samples. The color indicates the expression level of sRNAs (magenta indicates high, white indicates intermediate, and blue indicates low).</p

Morphology_Climate_data

Study sites, Morphological data and climatic conditions of the study sites

Comprehensive analysis of small RNAs expressed in developing male strobili of <i>Cryptomeria japonica</i>

<div><p>Deep sequencing of small RNAs (sRNAs) in developing male strobili of second-generation offspring originating from a nuclear genic male sterile tree of <i>Cryptomeria japonica</i> were performed to characterize sRNA populations in the male strobili at early pollen developmental stages. Comparing to sequences of microRNA (miRNA) families of plant species and sRNAs expressed in the reproductive organs of representative vascular plants, 37 conserved miRNA families were detected, of which eight were ubiquitously expressed in the reproductive organs of land plant species. In contrast, miR1083 was common in male reproductive organs of gymnosperm species but absent in angiosperm species. In addition to conserved miRNAs, 199 novel miRNAs candidates were predicted. The expression patterns of the obtained sRNAs were further investigated to detect the differentially expressed (DE) sRNAs between genic male sterile and fertile individuals. A total of 969 DE sRNAs were obtained and only three known miRNA families were included among them. These results suggest that both conserved and species-specific sRNAs contribute to the development of male strobili in <i>C</i>. <i>japonica</i>.</p></div

Migrate_infile_Weevil

The weevil mt COI data for the Migrate-n analysis

The small RNAs (sRNAs) related to the predicted repeat sequences of <i>Cryptomeria japonica</i>.

<p>The small RNAs (sRNAs) related to the predicted repeat sequences of <i>Cryptomeria japonica</i>.</p

Migrate_infile_Camellia

The camellia microsatellite data for the Migrate-n analysis

The numbers of DE sRNAs at each sampling date.

<p>The numbers of DE sRNAs at each sampling date.</p

Length distribution of <i>Cryptomeria japonica</i> small RNAs (sRNAs).

<p>Frequency of each size class is shown as a percentage of the total number of retained_sRNAs (black bars) and all unique sequences (white bars).</p