Comparative analysis of viral genome detection via real-time RT-PCR. Mean C_q values from duplicate runs.

<p>RABV: <i>Rabies virus</i>; ;EBLV: <i>European Bat Lyssavirus</i>;neg.: negative control; −: negative result; #: cross-reactivitiy with other <i>Lyssavirus</i> species; ?: doubtful result;</p>*<p>doubtful result was retested; i.h.: in-house assay; dilution series (0), (I), (II), (III); 10⁰, 10⁻¹, 10⁻², 10⁻³; mod.: assay modified; r: RABV-specific detection; e1: EBLV-1 specific; e1+2: EBLV-1+−2 specific; r13, r14, r13/14: R13, R14, duplex R13/14 assay by <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058372#pone.0058372-Hoffmann1" target="_blank">[17]</a>; <i>fn</i>: false negative results; <b>exp</b>: expected negative results from previous publication; ts: two-step systems; no duplicates for assays D2, D3 and M; 2) Orlowska et al., 2008 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058372#pone.0058372-Orlowska1" target="_blank">[22]</a>.</p

Mean quantification cycle (C_q) values of viral genome detection via EBLV-1 and EBLV-2 specific real-time RT-PCR.

<p>RABV: <i>Rabies virus</i>;;EBLV: <i>European Bat Lyssavirus</i>;neg.: negative control; –: not applicable; #: cross–reactivitiy with other <i>Lyssavirus</i> species; ?: inconclusive based on curve shape; dilution series (0), (I), (II), (III); 10⁰, 10⁻¹, 10⁻², 10⁻³;</p>*<p>Hoffmann and Müller personal communication; ts: two-step systems; no duplicates for assays M1 and M2; all laboratories except J1 used separate species-specific real-time PCRs to detect EBLV-1 or -2.</p