Distribution of the correct results of laboratories participating to the EQAs for RVF antibodies detection (IgG and IgM).

<p>Group 1 includes laboratories, which correctly classified 15 out of the 15 tested samples, Group 2 represents the laboratory failing to one test result. For IgG EQA group 1 includes the laboratories: #1, #2, #3, #4, #5, #6, #7a, #8, #9, #9a, #9b, #10, while in group 2 #7. For IgM EQA group 1 includes #1, #2, #4, #5, #6, #7, #7a, #8, #9, #9a, #9b, #10 and group 2 #3.</p

Results of the EQA for RVF IgM antibodies detection.

<p>Wild rum: wild ruminants (<i>Antidorcasmarsupialis</i>). Pos: RVF seropositive status; Neg: RVF seronegative status. + /—: samples identified as positive or negative by the participants. FP: false positive</p><p>^<i>†</i>IDvet: ID. Screen RVF IgM Capture</p><p>^<i>§</i><i>In-house assay</i>: test based on crude cell lysate as antigen</p><p>* Laboratory providing multiple datasets</p><p>Results of the EQA for RVF IgM antibodies detection.</p

EQA for RVFV virus genome detection, RNA extraction and RT PCR instruments and methods.

<p>na = not available information</p><p>* Laboratory providing multiple datasets</p><p>**Friedrich-Loeffler-Institut (in-house) using primer sequences published by Bird et al. (2007)</p><p>EQA for RVFV virus genome detection, RNA extraction and RT PCR instruments and methods.</p

Results of the EQA for RVF IgG antibodies detection.

<p>Wild rum: wild ruminants (<i>Antidorcas marsupialis</i>). Pos: RVF seropositive status; Neg: RVF seronegative status. + /—: samples identified as positive or negative by the participants. FP: false positive result.</p><p>^<i>(†)</i>IDvet: ID.Screen RVF competition multi- species kit.</p><p>^<i>(‡)</i>Ingenasa: Ingezim FVR DR 13-FVR.K0.</p><p>^<i>§</i><i>in house assay</i>: test based on crude cell lysate as antigen.</p><p>^{<i>§ §</i>}<i>in house assay</i>: test based on recombinant N protein as antigen.</p><p>* Laboratory providing multiple datasets</p><p>Results of the EQA for RVF IgG antibodies detection.</p

Results of the EQA for RVFV virus genome detection.

<p>Neg: RVFV seronegative bovine serum. + / -: samples identified as positive or negative by the participants. FP: false positive result. FN: false negative result. Inc: Inconclusive results.</p><p>* Laboratory providing multiple datasets</p><p>Results of the EQA for RVFV virus genome detection.</p

Results of the EQA for RVFV virus genome detection, cycle threshold (Ct) values.

<p>Neg: RVFV seronegative bovine serum. No Ct: result above the threshold.</p><p>* Laboratory providing multiple datasets</p><p>Results of the EQA for RVFV virus genome detection, cycle threshold (Ct) values.</p

Distribution of the correct results of laboratories participating to EQA for RVFV genome detection.

<p>Group 1 represents laboratories which correctly classified 15 out of the 15 tested samples (#1a, #7, #8, #9, #10). Group 2 represents the laboratory failing to one test result (#1). Group 3 represents the laboratory, which misidentified 5 test results (#2).</p