Detection of one <i>B. malayi</i> L3 added to pools of uninfected mosquitoes.

*<p>All samples were run in triplicate and average Ct values are shown.</p><p>Dashed lines indicate that the TC8100 RNA was not detected in the sample.</p

Specificity Testing of the <i>Brugia</i> L3 Detection Assay.

*<p>All samples were run in triplicate and average Ct values are shown.</p><p><i>BmL3</i> = <i>B. malayi L3</i>, <i>DiL3</i> = <i>D. immitis L3</i>, <i>WbL3</i> = <i>W. bancrofti L3</i>, <i>BpL3</i> = <i>B. pahangi L3 tph-1</i> is a constitutive target that is transcribed in all stages of <i>Brugia and Wuchereria</i>.</p><p>TC8100 is an L3-activated transcript that is only present in <i>Brugia</i> L3.</p><p>PBM = days post blood meal.</p><p>Dashed lines indicate that the RNA target was not detected in the sample.</p

cDNA Library PCR Screening of Gene Candidates.

<p>Gene Identifier = TIGR Cluster Number (TC) available at <a href="http://compbio.dfci.harvard.edu/tgi/cgi-bin/tgi/gimain.plgudbb_malayi" target="_blank">http://compbio.dfci.harvard.edu/tgi/cgi-bin/tgi/gimain.plgudbb_malayi</a>.</p><p>+ = PCR product detected, blank = no PCR product detected, and NT = not tested.</p><p><i>B. malayi</i> cDNA library abbreviations: BmMf = microfilarial stage, BmL2 = L2 stage, BmL3 = vector-derived L3 stage, BmL3d6 = L3 larvae collected 6 days post mammalian infection, BmL4 = mammalian derived L4 stage.</p

qRT-PCR Detection of the TC8100 Expression Profile Across a Two-Week Mosquito Time Course.

<p># dPBM = number of days post blood meal (after mosquitoes were fed on infected blood).</p>*<p>ratio of infected mosquitoes to total pool size.</p><p>No RNA indicates a faulty RNA Extraction as detected by spectrophotometry.</p><p>Neg <i>tph-1</i> indicates no parasite RNA was extracted in that mosquito pool.</p><p>“-” indicates no TC8100 RNA was detected in that sample.</p><p>Ct value = cycle threshold value (product amplification detected to cross the threshold).</p

Detection of a single infective mosquito in mosquito pools.

*<p>All samples were run in triplicate and average Ct values are shown.</p><p>Dashed lines indicate samples where no <i>B. malayi</i> RNA was detected.</p

Filariasis infection parameters by gender in Usino villages just prior to the first round of mass drug administration.

<p>Abbreviations: Mf, microfilaremia; CFA, circulating filarial antigenemia; Bm14, antibodies to recombinant filarial antigen Bm14.</p><p>Data shown are the number of positive tests and the total number of tests performed (% positive). Numbers in the first column refer to age in years.</p

Filarial antigenemia and Bm14 antibody rates in subjects with microfilaremia before and after mass drug administration (MDA).

<p>Data shown are the number of subjects with positive tests and the number of Mf-positive subjects tested (% positive) before the first round of MDA and approximately 12 months after each round of MDA.</p><p>Abbreviations: Mf, microfilaremia; CFA, circulating filarial antigenemia; Bm14, IgG₄ antibodies to recombinant filarial antigen Bm14.</p

Filariasis infection parameters by age in Usino villages just prior to the first round of mass drug administration.

<p>Abbreviations: Mf, microfilaremia; CFA, circulating filarial antigenemia; Bm14, antibodies to recombinant filarial antigen Bm14.</p><p>Data shown are the number of positive tests and the total number of tests performed (% positive).</p

Mosquito collections and filarial DNA rates in mosquitoes before and after mass drug administration (MDA).

<p>Filarial DNA was detected in mosquitoes by PCR, and rates (maximum likelihood with 95% CI) were estimated by PoolScreen. The filarial DNA rate in mosquitoes collected in the MDA-3 survey (6 to 9 months after the third round of MDA) represented a 92.3% decrease from the rate in mosquitoes collected during the pre-MDA-B survey (just prior to the first round of MDA).</p

Mass drug administration (MDA) compliance rates in Usino, Papua New Guinea.

<p>Mass drug administration (MDA) compliance rates in Usino, Papua New Guinea.</p