Identification of mouse myoepithelial markers in C57BL/6J MMTV-PyMT mammary gland tissue.

<p>Sections of formalin-fixed, paraffin-embedded C57BL/6J MMTV-PyMT mammary gland tissue were stained with Ki67 and a panel of myoepithelial markers and visualized with DAB (brown staining). All sections were counterstained with hematoxylin. Tissue sections containing normal ducts, hyperplastic, MIN and invasive regions (determined by a pathologist) were compared to determine the clearest myoepithelial markers for mouse tissue. Scale bar represents 50 μm.</p

Scoring SMMHC and CK14 expression to distinguish MIN from invasive lesions in C57BL/6J MMTV-PyMT mice.

<p>The expression of SMMHC and CK14 in myoepithelial cells were scored and averaged from independent areas from five mice. Two-sided Fisher’s exact test, p = 0.0031 (**p<0.01) <i>n</i> = 5 mice.</p

Expression of myoepithelial markers and Ki67 in C57BL/6J MMTV-PyMT mice.

<p>Expression of myoepithelial markers and Ki67 in C57BL/6J MMTV-PyMT mice.</p

Myoepithelial markers on mouse mammary gland tissue.

<p>Sections of formalin-fixed, paraffin-embedded Balb/c and C57BL/6J mammary gland tissue were stained with a panel of myoepithelial markers, smooth muscle actin (SMA), smooth muscle myosin heavy chain (SMMHC) and cytokeratin 14 (CK14) and visualized with DAB (brown staining). All sections were counterstained with hematoxylin. Scale bar represents 50 μm.</p

Scoring matrix to distinguish different tumour stages in C57BL/6J MMTV-PyMT mice.

<p>Scoring matrix to distinguish different tumour stages in C57BL/6J MMTV-PyMT mice.</p