2 research outputs found
Endophytic colonization and <i>in planta </i>nitrogen fixation by a diazotrophic <i>Serratia </i>sp. in rice
802-807Nitrogen fixing endophytic Serratia sp.
was isolated from rice and characterized. Re-colonization ability of Serratia
sp. in the rice seedlings as endophyte was studied under laboratory condition.
For detecting the re-colonization potential in the rice seedlings, Serratia sp.
was marked with reporter genes (egfp and Km′) using
transposon mutagenesis. The conjugants were screened for re-colonization
ability and presence of nif genes using PCR. Further, the influence of
flavonoids and growth hormones on the endophytic colonization and in planta nitrogen
fixation of Serratia was also investigated. The flavonoids, quercetin
(3ÎĽg /ml) and diadzein (2 ÎĽg/ml) significantly increased the re-colonization
ability of the endophytic Serratia, whereas the growth hormones like IAA
and NAA (5 ÎĽg/ml) reduced the endophytic colonization ability of Serratia
sp. Similarly, the in planta nitrogen fixation by Serratia sp. in
rice was significantly increased due to flavonoids. The inoculation of
endophytic diazotrophs increased the plant biomass and biochemical constituents
Bacterial Endophytes of Plants: Diversity, Invasion Mechanisms and Effects on the Host
Plant inner tissues are colonized by bacterial organisms known as endophytes. The relatively recent application of culture independent and molecular high throughput techniques allowed the description of a large diversity of endophytic bacterial taxa. These microorganisms can be found in any plant organ, including fruits and legume nodules. Some endophytic bacteria benefit the host by several mechanisms, and their application to economically important crops represents an interesting alternative to the use of agrochemicals. However, more studies are required to clearly assess their effects on the hosts (especially in co-inoculation with other beneficial bacteria) and the molecular events that lead to the interaction between plants and endophytic microorganisms. In this chapter, we focus on bacterial endophytes from legumes and non-legumes plants, analyzing their diversity and effects on the hosts. We also discuss the endophytic colonization of legume nodules, with emphasis on the endophytic bacterial diversity, the mechanisms involved in the nodule invasion and their effects on the hosts.Fil: Ibañez, Fernando Julio. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas, FisicoquĂmicas y Naturales. Departamento de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba; ArgentinaFil: Tonelli, Maria Laura. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba; Argentina. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas, FisicoquĂmicas y Naturales. Departamento de Ciencias Naturales; ArgentinaFil: Muñoz, Vanina Laura. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas, FisicoquĂmicas y Naturales. Departamento de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba; ArgentinaFil: Figueredo, MarĂa Soledad. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas, FisicoquĂmicas y Naturales. Departamento de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba; ArgentinaFil: Fabra, Adriana Isidora. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas, FisicoquĂmicas y Naturales. Departamento de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba; Argentin