2,310 research outputs found
Mosquito-borne parasites in the Great Plains: Searching for vectors of nematodes and avian malaria parasites
Vector-borne diseases in the United States have recently increased as a result of the changing nature of vectors, hosts, reservoirs, parasite/pathogens, and the ecological and environmental conditions. While most focus has been on mosquito-borne pathogens affecting humans, little is known regarding parasites of companion animal, livestock and wildlife and their potential mosquito hosts in the United States. This study assessed the prevalence of mature infections of Dirofilaria immitis and avian malaria parasites (Haemosporida) within urban mosquito (Diptera, Culicidae) communities in Oklahoma. 2,620 pools consisting of 12,686 mosquitoes from 13 species collected over two summers were tested for the presence of filarioid and haemosporidian DNA. Dirofilaria immitis-infected mosquitoes were detected only in Aedes albopictus (MIR=0.18-0.22) and Culex pipiens complex (MIR=0.12) collected in cities in central and southern Oklahoma. Two other filarioid nematode species with 91-92% similarity with Onchocerca spp. and Mansonella spp. were also detected. Haemosporidian DNA was detected in 13 mosquito pools (0.9% of pools tested) from seven mosquito species out of 13 species tested. Plasmodium DNA in four species (Cx. coronator, Cx. pipiens complex, Cx. tarsalis, and Psorophora columbiae) had high homology with published sequences of avian Plasmodium species while DNA in four other species (Cx. nigripalpus, Ps. columbiae, Anopheles quadrimaculatus, and An. punctipennis) were closely related to Plasmodium species from deer. One pool of Cx. tarsalis was positive with a 100% sequence identity of Haemoproteus sacharovi. This study provides a baseline concerning the diversity of parasites in different mosquito species present in the southern Great Plains. These studies provide important information for understanding the factors of transmission involving the mosquito community, potential hosts, and different mosquito-borne parasites in this important region involved in livestock management and wildlife conservation.Peer reviewedEntomology and Plant Patholog
Landscape and anthropogenic factors associated with adult Aedes aegypti and Aedes albopictus in small cities in the southern Great Plains
As mosquito-borne diseases are a growing human health concern in the United States, the distribution and potential arbovirus risk from container-breeding Aedes mosquitoes is understudied in the southern Great Plains. The aim of the study was to assess landscape and anthropogenic factors associated with encountering adult container-breeding mosquitoes in small cities in southern Oklahoma. Collections were carried out over a 10 week period from June to August 2017 along two geographical transects, each consisting of three cities, equally distant from the Red River/Texas border. Mosquitoes were collected weekly using two trap types along with data for 13 landscape, vegetation, and anthropogenic variables. After five rounds of collection, 6628 female mosquitoes were collected over 2110 trap-nights involving 242 commercial or residential sites in six cities. Of the mosquitoes collected, 80% consisted of container-breeding species: Aedes albopictus (72%), Culex pipiens complex (16%) and Aedes aegypti (8%). Regionally, Aedes aegypti was more likely present in cities closest to the Texas border while Ae. albopictus was spread throughout the region. In general, Ae. aegypti and Ae. albopictus were significantly more present in sites featuring no or low vegetation and residential sites. Variables associated with Ae. albopictus presence and abundance varied between cities and highlighted the urban nature of the species. The study highlighted the distribution of Ae. aegypti geographically and within the urban context, indicated potential habitat preferences of container-breeding mosquito species in small towns, and demonstrated the usefulness of Gravid Aedes traps (GAT) traps for monitoring Aedes populations in urban habitats in small cities.Peer reviewedEntomology and Plant Patholog
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Non-Histaminergic Itch Mediators Elevated in the Skin of a Porcine Model of Scabies and of Human Scabies Patients
Genotype-by-environment interactions govern fitness changes associated with adaptive mutations in two-component response systems
Introduction: Two-component response systems (TCRS) are the main mechanism by which prokaryotes acclimate to changing environments. These systems are composed of a membrane bound histidine kinase (HK) that senses external signals and a response regulator (RR) that activates transcription of response genes. Despite their known role in acclimation, little is known about the role TCRS play in environmental adaptation. Several experimental evolution studies have shown the acquisition of mutations in TCRS during adaptation, therefore here we set out to characterize the adaptive mechanism resulting from these mutations and evaluate whether single nucleotide changes in one gene could induce variable genotype-by-environment (GxE) interactions.Methods: To do this, we assessed fitness changes and differential gene expression for four adaptive mutations in cusS, the gene that encodes the HK CusS, acquired by Escherichia coli during silver adaptation.Results: Fitness assays showed that as the environment changed, each mutant displayed a unique fitness profile with greatest fitness in the original selection environment. RNAseq then indicated that, in ± silver nitrate, each mutant induces a primary response that upregulates cusS, its RR cusR, and constitutively expresses the target response genes cusCFBA. This then induces a secondary response via differential expression of genes regulated by the CusR through TCRS crosstalk. Finally, each mutant undergoes fitness tuning through unique tertiary responses that result in gene expression patterns specific for the genotype, the environment and optimized for the original selection conditions.Discussion: This three-step response shows that different mutations in a single gene leads to individualized phenotypes governed by unique GxE interactions that not only contribute to transcriptional divergence but also to phenotypic plasticity
Quantum-circuit design for efficient simulations of many-body quantum dynamics
We construct an efficient autonomous quantum-circuit design algorithm for
creating efficient quantum circuits to simulate Hamiltonian many-body quantum
dynamics for arbitrary input states. The resultant quantum circuits have
optimal space complexity and employ a sequence of gates that is close to
optimal with respect to time complexity. We also devise an algorithm that
exploits commutativity to optimize the circuits for parallel execution. As
examples, we show how our autonomous algorithm constructs circuits for
simulating the dynamics of Kitaev's honeycomb model and the
Bardeen-Cooper-Schrieffer model of superconductivity. Furthermore we provide
numerical evidence that the rigorously proven upper bounds for the simulation
error here and in previous work may sometimes overestimate the error by orders
of magnitude compared to the best achievable performance for some
physics-inspired simulations.Comment: 20 Pages, 6 figure
Delivering alcohol identification and brief advice (IBA) in housing settings: a step too far or opening doors?
Within the UK, there is a drive to encourage the delivery of alcohol screening (or identification) and brief advice (IBA) in a range of contexts beyond primary care and hospitals where the evidence is strongest. However, the evidence base for effectiveness in non-health contexts is not currently established. This paper considers the case of housing provided by social landlords, drawing on two research studies which were conducted concurrently. One study examined the feasibility of delivering alcohol IBA in housing settings and the other the role of training in delivering IBA in non-health contexts including housing. This paper draws mainly on the qualitative data collected for both studies to examine the appropriateness and feasibility of delivering IBA in a range of social housing settings by the housing workforce. Findings suggest that while it is feasible to deliver IBA in housing settings, there are similar challenges and barriers to those already identified in relation to primary care. These include issues around role inadequacy, role legitimacy and the lack of support to work with people with alcohol problems. Results indicate that the potential may lie in focusing training efforts on specific roles to deliver IBA rather than it being expected of all staff
Sustained antigen availability during germinal center initiation enhances antibody responses to vaccination
Natural infections expose the immune system to escalating antigen and inflammation over days to weeks, whereas nonlive vaccines are single bolus events. We explored whether the immune system responds optimally to antigen kinetics most similar to replicating infections, rather than a bolus dose. Using HIV antigens, we found that administering a given total dose of antigen and adjuvant over 1–2 wk through repeated injections or osmotic pumps enhanced humoral responses, with exponentially increasing (exp-inc) dosing profiles eliciting >10-fold increases in antibody production relative to bolus vaccination post prime. Computational modeling of the germinal center response suggested that antigen availability as higher-affinity antibodies evolve enhances antigen capture in lymph nodes. Consistent with these predictions, we found that exp-inc dosing led to prolonged antigen retention in lymph nodes and increased Tfh cell and germinal center B-cell numbers. Thus, regulating the antigen and adjuvant kinetics may enable increased vaccine potency.National Institute of Allergy and Infectious Diseases (U.S.) (Awards UM1AI100663)National Institute of Allergy and Infectious Diseases (U.S.) (Awards AI110657
Spin half fermions with mass dimension one: theory, phenomenology, and dark matter
We provide the first details on the unexpected theoretical discovery of a
spin-one-half matter field with mass dimension one. It is based upon a complete
set of dual-helicity eigenspinors of the charge conjugation operator. Due to
its unusual properties with respect to charge conjugation and parity, it
belongs to a non-standard Wigner class. Consequently, the theory exhibits
non-locality with (CPT)^2 = - I. We briefly discuss its relevance to the
cosmological `horizon problem'. Because the introduced fermionic field is
endowed with mass dimension one, it can carry a quartic self-interaction. Its
dominant interaction with known forms of matter is via Higgs, and with gravity.
This aspect leads us to contemplate the new fermion as a prime dark matter
candidate. Taking this suggestion seriously we study a supernova-like explosion
of a galactic-mass dark matter cloud to set limits on the mass of the new
particle and present a calculation on relic abundance to constrain the relevant
cross-section. The analysis favours light mass (roughly 20 MeV) and relevant
cross-section of about 2 pb. Similarities and differences with the WIMP and
mirror matter proposals for dark matter are enumerated. In a critique of the
theory we bare a hint on non-commutative aspects of spacetime, and
energy-momentum space.Comment: 78 pages [Changes: referee-suggested improvements, additional
important references, and better readability
A Nonlocal Metric Formulation of MOND
We study a class of nonlocal, but causal, covariant and conserved field
equations for the metric. Although nonlocal, these equations do not seem to
possess extra graviton solutions in weak field perturbation theory. Indeed, the
equations reduce to those of general relativity when the Ricci scalar vanishes
throughout spacetime. When a static matter source is present we show how these
equations can be adjusted to reproduce Milgrom's Modified Newtonian Dynamics in
the weak field regime, while reducing to general relativity for strong fields.
We compute the angular deflection of light in the weak field regime and
demonstrate that it is the same as for general relativity, resulting in far too
little lensing if no dark matter is present. We also study the field equations
for a general Robertson-Walker geometry. An interesting feature of our
equations is that they become conformally invariant in the MOND limit.Comment: 22 pages, LaTeX 2 epsilon, no figure
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