MOLECULAR ASSESSMENT OF GENETIC DIVERSITY IN INDIAN ACCESSIONS OF ALOE VERA USING SSR MARKER

Objective: In this study Aloe vera (L.) Burm. f. collected from 12 states covering all the different agro-climatic zones of India were investigated for its genetic diversity analysis by using SSR marker assay.Methods: Total genomic DNA was isolated from young leaf samples using CTAB method. Twenty primers were selected which were used for Asparagus officinalis L a related species of A. vera and others were developed from available Aloe vera plant sequences with the help of primer 3 software. Similarity matrices and dendrogram were constructed by using NTSys software to show a phenetic representation of the genetic relationship. Polymorphic Information Content (PIC), the effective multiplex ratio (EMR) and Marker Index (MI) were calculated for the assessment of genetic diversity.Results: The neighbor-joining tree based on all SSR fragments of twelve Aloe vera germplasm accessions grouped into three major clusters. The similarity value ranged from 46 % to 100 %. The highest 100 % similarity was noted between Haryana and Uttar Pradesh accessions followed by 93% similarity between Haryana and Punjab accessions with Rajasthan. Minimum similarity was noted between Gujarat and Kerala accessions.Conclusion: This study revealed the rich genetic diversity among Aloe vera accessions from different agro-climatic zones of India. It is also concluded that SSR marker analysis can be a useful tool for the assessment of genetic diversity of the medicinal plants.Â

OVERLAPPED CLUSTERING APPROACH FOR MAXIMIZING THE SERVICE RELIABILITY OF HETEROGENEOUS DISTRIBUTED COMPUTING SYSTEMS

ABSTRACT For distributed computing system (DCS) where server nodes can fail permanently with nonzero probability, the reliability of the system can be defined as the probability that the system run the entire tasks successfully assign on it before all the nodes fail. In heterogeneous distributed system where various nodes of the system have different characteristics, reliability of the system is highly dependent on the tasks allocation strategies. So, this paper presents a rigorous framework for efficient tasks allocation in heterogeneous distributed environment, with the goal of maximizing the system reliability. Reliability of the system is characterized in the presence of communication uncertainties and topological changes due to node's failure. Node failure has adverse effects on the system reliability. Thus, one possible way to improve reliability is to make the communication among the tasks as local as possible. For this, an overlapped clustering approach has been used. Further, we calculate the reliability of each node of the DCS to determine the actual capabilities of each node. Here, our purpose is to assign the more costly task to more reliable node of the DCS. Then we utilize the load balancing policies for handling the node's failure effect as well as maximizing the service reliability of the DCS. A numeric example is presented to illustrate the importance of incorporating overlapping cluster and load balancing on the reliability study

Assessment of various cognitive domains in patient with obsessive-compulsive disorder

Background: OCD is often chronic disorder and causes impairment of functional efficiency. Various researchers have found that cognitive dysfunction frequently present even when mild symptoms are present. This study aimed to investigate various domains of cognitive function in patients with OCD using cross sectional design. In this study we have tried to find out the various domains of cognitive function in OCD and their relation with clinical severities. We also compared the cognitive function of patients with OCD and their control. Methods: Total 60 subjects (30 in case group and 30 in control group) were taken for study. The study subjects (OCD group) were taken from psychiatric OPD and control group from general populations. The subjects of both the groups were screened as per inclusion and exclusion criteria and the Y-BOCS, ACE –III scales were applied. Results: In the case group, 96.70% had a normal ACE-III score and 3.30% had an inconclusive score, while in the control group 100% had a normal score at ACE-III scale, on further scoring. 60% patients have moderate and 33.3% have severe score in the YBOCS scale. We have found the significant decline in total mean ACE-III scores between case and control group (p value 0.000) and decline in memory domain with severity of illness was also significant (p value 0.037). Conclusions: Decline in cognition domain of memory in patient with OCD, was significant in comparison to control group

Physico-chemical analysis of selected ground water samples of Agra city, India

The aim of present study was carried out to assess the status of the groundwater in Agra city .The range of physicochemical parameters like pH (7.2-7.7), EC (1580-5200 mmhos), TDS (1020-4950 mg/l), Turbidity (1.1-31.4 NTU), Total Alkalinity (330-525 mg/l), Total hardness 240-1425 mg/l), Chloride (295-1140 mg/l), Calcium (72-436 mg/l), Magnesium 14.6-151.2 mg/l), Sodium (126.5-1254.9 mg/l) and Potassium (1.9-60.6 mg/l) were found to be higher than the natural background level of groundwater. This indicates the groundwater pollution in selected water samples from 12 sampling sites from Feb. to May 2011 of Agra city. The results considered that the groundwater of the study area in general cannot be considered as good quality

On the Effective throughput of Shadowed Beaulieu-Xie fading channel

Given the imperative for advanced wireless networks in the next generation and the rise of real-time applications within wireless communication, there is a notable focus on investigating data rate performance across various fading scenarios. This research delved into analyzing the effective throughput of the shadowed Beaulieu-Xie (SBX) composite fading channel using the PDF-based approach. To get the simplified relationship between the performance parameter and channel parameters, the low-SNR and the high-SNR approximation of the effective rate are also provided. The proposed formulations are evaluated for different values of system parameters to study their impact on the effective throughput. Also, the impact of the delay parameter on the EC is investigated. Monte-Carlo simulations are used to verify the facticity of the deduced equations.Comment: 18 page

Single Stage Management of Anorectal Malformation in Male Neonates: Experience of a Tertiary Care Centre

Introduction: Anorectal malformation [ARM] can be treated either by staged procedures or by a single stage procedure. In the present study we have performed single stage surgery of ARM in male neonates. Materials and Methods: Retrospective review of cases of ARM operated over 4 years at Indira Gandhi Institute of Medical Sciences, Patna, and Bihar from January 2015 to December 2018 were undertaken. In this study we have compared the results of single stage repair of Anorectal malformation with that of staged procedures performed earlier. Fifty four patients of Anorectal malformation were operated by single stage procedure (category 1) this was compared with staged procedures (category 2). Forty three patients underwent primary PSARP (posterior sagittal anorectoplasty) and the remaining 11 primary APP (abdomino-perineal pull through) procedure. The result of these single stage procedures were compared with that of staged procedures (category 2) in which a total of 39 cases were operated from 2012 to 2014. Result: Mean post-operative hospital stay in category 1 was 11 days and 32 days in category 2. In category 1, 20 patients were analysed. In which PSARP procedure was done in 15 and in 5 patients APP procedure was performed. The Rest of the patients are in follow up. Kelly score was good to fair in 17 patients (85%) and poor in 3 (15%) patients. In category 2 it was good to fair in 28 (71.7%) cases and poor in 11 cases (28%). Conclusion: Single stage surgical repair of anorectal malformation in male neonates is reliable and can be safely performed. Early results are encouraging; however, a long term follow-up is required to get any definite conclusion

Association of serum beta-hCG and urine albumin-creatinine ratio with hypertensive disorders during pregnancy

Background: Preeclampsia with or without severe symptoms, chronic hypertension with or without superimposed pre-eclampsia/eclampsia, gestational hypertension, HELLP syndrome, or eclampsia all pose a significant risk of morbidity for both the mother and the unborn child. The aim of this study was to investigate if the albumin-to-creatinine ratio in urine spots and the serum beta-hCG level correlate with the hypertensive illness of pregnancy.” Method: “The current inquiry was conducted during the months of October 2020 and August 2022 at the obstetrics and gynaecology department of Subharti medical college in Meerut, Uttar Pradesh. The study was not open to women who were more than 20 weeks pregnant, had gestational diabetes mellitus, had more than one pregnancy, had chronic hypertension, chronic renal disease, chronic liver disease, cardiac disease, systemic lupus erythematosus, or haematological illnesses. We measured the levels of serum beta hCG and the urine albumin-creatinine ratio, and we compared them between the groups.” Results:  After ensuring that each participant had given their informed permission, the trial comprised a total of 200 patients. In the hypertensive group of the participants in the study, 31% were between the ages of 21 and 35, and 29% were less than 20 years old. With increased beta hCG and UACR, there was a higher incidence of hypertensive retinopathy, acute renal failure, DIC, and PPH among the patients, as well as the poorest fetal outcomes. (p<0.05) Conclusions: The presence of a substantial relationship between hypertensive diseases and raised levels of beta-hCG during pregnancy, as well as a greater ratio of urine albumin to creatinine.  There is a considerable increase in the incidence of fetal growth retardation, preterm, and mortality occurring within the uterus among mothers who have higher levels of beta-hCG and urine ACR

Comparison of Magnesium Sulphate and Dexmedetomidine for Attenuation of Stress Response in Patients undergoing Laparoscopic Cholecystectomy under General Anaesthesia by Measuring Biochemical Markers of Stress Response: A Prospective Randomized Study

Background: Laryngoscopy, endotracheal intubation, pneumoperitoneum creation and extubation is stressful event marked by hemodynamic changes during laparoscopic procedures. This increases secretion of many biochemical stress markers for example, cortisol levels, TNF-alpha levels, CRP levels, blood Sugar levels. Aim of the study was to compare magnesium sulphate and dexmedetomidine for attenuation of stress response in patients undergoing laparoscopic cholecystectomy under general anaesthesia by measuring biochemical markers of stress response. Methods: 60 patients, age between 18 to 60 years of either sex, who were undergoing Laparoscopic cholecystectomy randomized into two groups of 30 patients each by computer generated random number. Group M- received magnesium sulphate 50 mg/kg and group D -  received dexmedetomidine 1 µg/kg. Results: The demographic data were comparable in both groups. Cortisol levels rise in both the groups but significantly more in group M than group D at 30 minute (p-value < 0.001) and 4 hours (p-value < 0.001). CRP levels rise in both the groups but significantly more in group M than group D at 30 min (p-value 0.013) and 4 hours (p-value 0.020). Blood sugar levels rise in both the groups but significantly more in group M than group D at 30 min, 4 hours and 24 hours (p-value <0.001). TNF-alpha levels rise in both the groups but significantly more in group M than group D at 30 min (p-value 0.005) and 4 hours (p-value 0.007). Conclusion: We conclude that biochemical stress marker levels (Cortisol levels, TNF-alpha, levels, CRP levels, Blood Sugar levels) were more increased in the magnesium sulphate group compared with the dexmedetomidine group. Heart rate and Mean arterial pressure   were higher in the Magnesium sulphate group than the Dexmedetomidine group. Dexmedetomidine is better than magnesium sulphate in attenuating the stress of surgery in patients undergoing laparoscopic cholecystectomy