The Effect of Streptomycin on Freezing Rainbow Trout (Oncorhynchus mykiss) Sperm

Mature male and female rainbow trout (Oncorhynchus mykiss) were used to study the effect of different concentrations of streptomycin in a cryopreservation solution on the sperm motility, motility duration, and fertilization rate of rainbow trout. 300mM glucose and 10% DMSO containing diluent was used as a cryopreservation solution. Three different concentrations (3600, 7200 and 10800 μg/ml) of streptomycin were added to the diluent. Our study showed that various concentrations of streptomycin in cryopreservation solution caused a significant reduction in the percentage and duration of rainbow trout spermatozoa motility after freezing-thawing. The percentage of egg fertilization also decreased. When streptomycin-containing groups were compared with each other, a statistical difference was found (p<0.05). However, no statistical difference was observed in terms of the percentage of eyed-embryos

The Effect of Streptomycin on Freezing Rainbow Trout (Oncorhynchus mykiss) Sperm

Mature male and female rainbow trout (Oncorhynchus mykiss) were used to study the effect of different concentrations of streptomycin in a cryopreservation solution on the sperm motility, motility duration, and fertilization rate of rainbow trout. 300mM glucose and 10% DMSO containing diluent was used as a cryopreservation solution. Three different concentrations (3600, 7200 and 10800 &mu;g/ml) of streptomycin were added to the diluent. Our study showed that various concentrations of streptomycin in cryopreservation solution caused a significant reduction in the percentage and duration of rainbow trout spermatozoa motility after freezing-thawing. The percentage of egg fertilization also decreased. When streptomycin-containing groups were compared with each other, a statistical difference was found (p&lt;0.05). However, no statistical difference was observed in terms of the percentage of eyed-embryos

Effects of Different Doses of Taurine in the Glucose-Based Extender During Cryopreservation of Rainbow Trout ( Oncorhynchus Mykiss

This study aimed to investigate the effects of different doses of taurine added to glucose extender Semen was collected from rainbow trout (Oncorhynchus mykiss) by abdominal massage and diluted (1:2) with 300 mM glucose (G) extender containing different taurine doses (50, 75 and 100 mM). The control group extender did not contain taurine. After dilution and dosing, samples were filled to 0.5 ml straws, frozen in nitrogen vapour and stored in liquid nitrogen. The comparison of the results obtained for the 50 mM taurine group and the control group showed no significant difference between the post-thawing motility percentages and motility duration of sperm, and only a very low statistically difference for eyed-embryo percentages (P < 0.05)