Effects of high-intensity training with one versus three changes of direction on youth female basketball players’ performance

To compare the effects of high-intensity interval training (HIT) with one versus three changes of direction (COD) on young (age, 17.2±1.1 years) female basketball players’ performance, six weeks of regular basketball training (control period) was followed by six weeks of high-intensity training added to regular training , two times per week, with a random allocation of athletes to either HIT with one (HIT-COD1; n=6) or three COD (HIT-COD3; n=6). Before and after the control and HIT-COD training periods athletes performed repeatedsprint ability test (RSA), modified agility T-test (MAT), V-cut, triple standing dominant (TS-D) and nondominant (TS-ND) jump, TS-D and TS-ND with COD tests, and 30-15 Intermittent Fitness Test (30-15IFT). With the exception of a substantial improvement in V-cut in both groups, no substantial changes occurred during the control period. Both HIT-COD training programs improved V-cut, although only HIT-COD3 substantially improved RSA mean time (RSAm), MAT and the final speed reached in 30-15IFT (VIFT). The between-group comparison revealed greater improvements in RSAm and VIFT in HIT-COD3 than in HITCOD1. In conclusion, supplementation of basketball training with HIT-COD drills adds improvements to young female basketball player’s performance, especially when 3 COD are incorporated into HIT

Child Head Circumference and Placental MFSD2a Expression Are Associated to the Level of MFSD2a in Maternal Blood During Pregnancy

Gestational diabetes mellitus (GDM) is a world-wide health challenge, which prevalence is expected to increase in parallel to the epidemic of obesity. Children born from GDM mothers have lower levels of docosahexaenoic acid (DHA) in cord blood, which might influence their neurodevelopment. Recently, the membrane transporter Major Family Super Domain 2a (MFSD2a) was associated with the selective transportation of DHA as lysophospholipids. The expression of the DHA membrane transporter MFSD2a is lower in GDM placentas, which could affect materno-fetal DHA transport. Humans with homozygous inactivating mutations in the MFSD2a gene present severe microcephaly and intellectual impairments. Herein, we intended to identify early blood biomarkers that may be of use during pregnancy to monitor the offspring development and the adequate nutritional interventions, such as nutritional supplementation, that may be selected to improve it. We evaluated MFSD2a expression in maternal blood at the third trimester of pregnancy, and its potential relationship with the expression of placental MFSD2a at delivery and child outcomes. Three groups of pregnant women were recruited: 25 controls, 23 GDM with dietary treatment, and 20 GDM with insulin treatment. Maternal and neonatal anthropometric and biochemical parameters were evaluated. MFSD2a was analyzed in placenta, blood and serum. MFSD2a protein expression in maternal blood was significantly lower in GDM groups and correlated with placental MFSD2a and Z-score neonatal head circumference during the first 6 months of life. The cord/maternal serum ratio of DHA, a solid indicator of materno-fetal DHA transport, was reduced in GDM groups and correlated with MFSD2a in maternal blood at the third trimester and in placenta at delivery. This indicates that altered MFSD2a levels in maternal blood during pregnancy might influence placental nutrient transport and fetal neurodevelopment. Furthermore, MFSD2a levels in maternal blood on the third trimester were inversely correlated to DHA in maternal serum lyso-PL. Thus, the level of MFSD2a in maternal blood could be used as a potential biomarker for the early detection of disturbances of MFSD2a expression during pregnancy and the subsequent consequences for the neurodevelopment of the child, as well as it may help to choose the optimal treatment approach for the affected subjects

Determination of nitrophenols in environmental samples using stir bar sorptive extraction coupled to thermal desorption gas chromatography-mass spectrometry

This paper presents a procedure for the determination of seven nitrophenols (NPs) in water and soil samples using stir bar sorptive extraction (SBSE) coupled to gas chromatography with mass spectrometry (GC-MS) by means of a thermal desorption unit (TDU). Microwave assisted extraction (MAE) is proposed to release the NPs from the soil matrices into an aqueous phase, prior to their acetylation. The different variables affecting the preconcentration efficiency of SBSE, during both the adsorption and the thermal desorption steps, are studied. As regards the analytical characteristics of the method, the accuracy was measured through recovery studies, re covery percentages in all cases being in the 79–120% range, as well as by analyzing a certified reference ma terial. The precision was evaluated in terms of relative standard deviation, which provided values lower than 15% for both repeatability and reproducibility. The limits of detection were between 0.001 and 0.031 µg L−1 for water and 0.020–0.107 ng g−1 for soil samples. When environmental samples of different origins were analyzed, contents in the 0.01–1.0 µg L−1 and 0.7–40 ng g−1 ranges were obtained for waters and soils, respectively

Playful activities and board games for the Games Library and Information Science-UCM site

Los dispositivos electrónicos y los desarrollos tecnológicos se han convertido en activos para captar la atención del estudiante y fomentar su el interés. Por eso, la formación trata de utilizar las tecnologías para favorecer el aprendizaje de determinadas competencias o habilidades requeridas para el alumnado. El objetivo de este proyecto es desarrollar actividades educativas y lúdicas de carácter web que sirvan de entretenimiento y que permitan comprender y memorizar conceptos y materias de las distintas materias del área de la Biblioteconomía y la Documentación. Para la implementación de las actividades se utilizarán tecnologías web de HTML5 y web responsible basadas en aplicaciones y contenido enriquecido. Las actividades lúdicas se pondrán a disposición del alumnado en un espacio web que constituirá un portal de actividades lúdicas sobre la Biblioteconomía y la Documentación. Los juegos serán multilingües y orientados a la accesibilidad web y la integración de los perfiles de diversidad. Asimismo, se recogerán las herramientas de propuestas de juegos de mesa adaptados al área. El resultado por tanto son diferentes tipos de actividades lúdicas disponibles en un sitio web, organizadas por materias y recuperables por conceptos. La principal aportación de este proyecto es que supone un método de aprendizaje de refuerzo de forma amena y divertida con sesiones cortas y realizando actividades adaptadas al usuario. Adicionalmente destaca la difusión de titulaciones asociadas como los másteres y el grado de la Facultad de Ciencias de la Documentación y de la Universidad Complutense. Por último, la propuesta es tecnológicamente escalable tanto por las materias transversales como por la capacidad de incorporación de materias y actividades.Electronic devices and technological developments have become assets to capture students' attention and encourage their interest. Therefore, training tries to use technologies to promote the learning of certain competencies or skills required for students. The objective of this project is to develop educational and ludic activities of a web nature that serve as entertainment and that allow the understanding and memorization of concepts and subjects of the different areas of Library and Information Science and Documentation. For the implementation of the activities, HTML5 and web responsible technologies based on rich applications and content will be used. The playful activities will be made available to students in a web space that will constitute a portal of playful activities on Library and Information Science. The games will be multilingual and oriented to web accessibility and the integration of diversity profiles. It will also include the tools for proposing board games adapted to the field. The result is therefore different types of recreational activities available on a website, organized by subject and retrievable by concepts. The main contribution of this project is that it is a method of learning reinforcement in a pleasant and fun way with short sessions and performing activities adapted to the user. Additionally, the dissemination of associated degrees such as the masters and the degree of the Faculty of Documentation Sciences and the Complutense University stands out. Finally, the proposal is technologically scalable both for the transversal subjects and for the capacity to incorporate subjects and activities.Depto. de Biblioteconomía y DocumentaciónFac. de Ciencias de la DocumentaciónFALSEVicerrectorado de Calidad de la Universidad Complutense de Madridsubmitte

Milk fat globule membrane plus milk fat increase docosahexaenoicacid availability in infant formulas

©. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/ This document is the Published, version of a Published Work that appeared in final form in [European Journal of Nutrition]. To access the final edited and published work see[https://doi.org/10.1007/s00394-022-03024-5]Purpose Milk fat globule membrane (MFGM) has components with emulsifer properties that could afect the provision of substrates to the brain. We evaluated the efects of MFGM plus milk fat addition to infant formulas on docosahexaenoic acid (DHA) availability and gut development. Methods In Experiment 1, suckling piglets were divided into 3 groups: Group L1 (n=8): fed with a vegetal fat formula with palm oil; L2 (n=8): canola oil formula and L3 (n=8): milk fat+canola oil+1% Lacprodan (3% MFGM of total protein content). In Experiment 2, Group L4 (n=7): fed with canola oil+1% Lacprodan (3% MFGM) and Group L5 (n=5): milk fat+canola oil+2% Lacprodan (6% MFGM). All formulas contained 0.2% DHA and 0.2% arachidonic acid. Results In Experiment 1, DHA was similar among the groups in both total fatty acids and plasma phospholipids (PL). However, 3% MFGM (L3) increased signifcantly the proportion of DHA and LC-PUFA n-3 in liver total fatty acids, jejunum, and also in jejunum PL respect to the other formulas. There were no changes in gut histology, cell proliferation, apoptosis, or brain DHA content. In Experiment 2, higher MFGM dose was used. Then, higher DHA was not only found in peripheral tissues of 6% MFGM (L5) piglets but also in plasma PL, while a similar trend was observed in cortex PL (p=0.123). Conclusion In conclusion, MFGM plus milk fat may increase DHA availability of infant formulas which could contribute to their benefcial health efects

Development of an Information and Documentation Games Portal-UCM

El proyecto “Desarrollo de un Portal de Juegos Información y Documentación-UCM” es continuación anteriores proyectos que trabajaban de uno de los aspectos del proyecto “Desarrollo de un entorno de aprendizaje virtual aplicado a la búsqueda y gestión de información iniciado en del año 2016. El objetivo principal de este proyecto ha sido desarrollar un portal web de juegos y actividades lúdicas para que los alumnos de la UCM y usuarios internautas interesados. El sitio web se dirige al objetivo de entretener aprendiendo y favoreciendo memorizar de un modo lúdico los contenidos de diferentes temas asignaturas del Grado de Información y Documentación mediante el uso de dispositivos móviles.The project "Development of an Information and Documentation Games Portal-UCM" is a continuation of previous projects that worked on one of the aspects of the project "Development of a virtual learning environment applied to the search and management of information started in 2016. The main objective of this project has been to develop a web portal for games and recreational activities for UCM students and interested Internet users. The website is aimed at the objective of entertaining by learning and favoring memorizing in a playful way the contents of different subjects of the Information and Documentation Degree through the use of mobile devices.Depto. de Biblioteconomía y DocumentaciónFac. de Ciencias de la DocumentaciónFALSEVicerrectorado de Calidad de la Universidad Complutense de Madridsubmitte

Roles of active site residues in LodA, a cysteine tryptophylquinone dependent ε-lysine oxidase

Site-directed mutagenesis identified residues in the substrate channel of LodA that play multiple roles in regulating K(m) values of substrates, k(cat) and the extent of biosynthesis of the protein-derived cysteine tryptophylquinone (CTQ) cofactor. Mutations of Cys448 increase K(m) values for lysine and O(2), with the larger effect on K(lysine). Tyr211 resides within a mobile loop and is seen in the crystal structure of LodA to form a hydrogen bond with Lys530 that appears to stabilize its position in the channel. Y211F LodA had reduced levels of CTQ but near normal levels of k(cat). K530A and K530R variants exhibited diminished levels of CTQ but significantly increased k(cat). The Y211F, K530A and K530R mutations each caused large increases in the K(m) values for lysine and O(2). These effects of the mutations of Tyr211 and Lys530 suggest that when these residues are hydrogen-bonded they may form a gate that controls entry and exit of substrates and products from the active site. Y211A and Y211E variants had the highest level of CTQ but exhibited no activity. These results highlight the different evolutionary factors that must be considered for enzymes which possess protein-derived cofactors, in which the catalytic cofactor must be generated by posttranslational modifications

Child Head Circumference and Placental MFSD2a Expression Are Associated to the Level of MFSD2a in Maternal Blood During Pregnancy

©. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by /4.0/ This document is the Published Manuscript version of a Published Work that appeared in final form in [Frontiers in Endocrinology]. To access the final edited and published work see[10.3389/fendo.2020.00038