686 research outputs found
The Minimum-Uncertainty Squeezed States for for Atoms and Photons in a Cavity
We describe a six-parameter family of the minimum-uncertainty squeezed states
for the harmonic oscillator in nonrelativistic quantum mechanics. They are
derived by the action of corresponding maximal kinematical invariance group on
the standard ground state solution. We show that the product of the variances
attains the required minimum value 1/4 only at the instances that one variance
is a minimum and the other is a maximum, when the squeezing of one of the
variances occurs. The generalized coherent states are explicitly constructed
and their Wigner function is studied. The overlap coefficients between the
squeezed, or generalized harmonic, and the Fock states are explicitly evaluated
in terms of hypergeometric functions. The corresponding photons statistics are
discussed and some applications to quantum optics, cavity quantum
electrodynamics, and superfocusing in channeling scattering are mentioned.
Explicit solutions of the Heisenberg equations for radiation field operators
with squeezing are found.Comment: 27 pages, no figures, 174 references J. Phys. B: At. Mol. Opt. Phys.,
Special Issue celebrating the 20th anniversary of quantum state engineering
(R. Blatt, A. Lvovsky, and G. Milburn, Guest Editors), May 201
A 3D Map of the Human Genome at Kilobase Resolution Reveals Principles of Chromatin Looping
SummaryWe use in situ Hi-C to probe the 3D architecture of genomes, constructing haploid and diploid maps of nine cell types. The densest, in human lymphoblastoid cells, contains 4.9 billion contacts, achieving 1 kb resolution. We find that genomes are partitioned into contact domains (median length, 185 kb), which are associated with distinct patterns of histone marks and segregate into six subcompartments. We identify ∼10,000 loops. These loops frequently link promoters and enhancers, correlate with gene activation, and show conservation across cell types and species. Loop anchors typically occur at domain boundaries and bind CTCF. CTCF sites at loop anchors occur predominantly (>90%) in a convergent orientation, with the asymmetric motifs “facing” one another. The inactive X chromosome splits into two massive domains and contains large loops anchored at CTCF-binding repeats.PaperFlic
Spin- and energy relaxation of hot electrons at GaAs surfaces
The mechanisms for spin relaxation in semiconductors are reviewed, and the
mechanism prevalent in p-doped semiconductors, namely spin relaxation due to
the electron-hole exchange interaction, is presented in some depth. It is shown
that the solution of Boltzmann-type kinetic equations allows one to obtain
quantitative results for spin relaxation in semiconductors that go beyond the
original Bir-Aronov-Pikus relaxation-rate approximation. Experimental results
using surface sensitive two-photon photoemission techniques show that the spin
relaxation-time of electrons in p-doped GaAs at a semiconductor/metal surface
is several times longer than the corresponding bulk spin relaxation-times. A
theoretical explanation of these results in terms of the reduced density of
holes in the band-bending region at the surface is presented.Comment: 33 pages, 12 figures; earlier submission replaced by corrected and
expanded version; eps figures now included in the tex
Principles of meiotic chromosome assembly revealed in S. cerevisiae
During meiotic prophase, chromosomes organise into a series of chromatin loops emanating from a proteinaceous axis, but the mechanisms of assembly remain unclear. Here we use Saccharomyces cerevisiae to explore how this elaborate three-dimensional chromosome organisation is linked to genomic sequence. As cells enter meiosis, we observe that strong cohesin-dependent grid-like Hi-C interaction patterns emerge, reminiscent of mammalian interphase organisation, but with distinct regulation. Meiotic patterns agree with simulations of loop extrusion with growth limited by barriers, in which a heterogeneous population of expanding loops develop along the chromosome. Importantly, CTCF, the factor that imposes similar features in mammalian interphase, is absent in S. cerevisiae, suggesting alternative mechanisms of barrier formation. While grid-like interactions emerge independently of meiotic chromosome synapsis, synapsis itself generates additional compaction that matures differentially according to telomere proximity and chromosome size. Collectively, our results elucidate fundamental principles of chromosome assembly and demonstrate the essential role of cohesin within this evolutionarily conserved process
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A comparison of shearing strengths of glued joints at various grain directions as determined by four methods of test
Information reviewed and reaffirmed March 1956
Fluorescent Lifetime Trajectories of a Single Fluorophore Reveal Reaction Intermediates During Transcription Initiation
Integrating transposable elements in the 3D genome
Chromosome organisation is increasingly recognised as an essential component of genome regulation, cell fate and cell health. Within the realm of transposable elements (TEs) however, the spatial information of how genomes are folded is still only rarely integrated in experimental studies or accounted for in modelling. Whilst polymer physics is recognised as an important tool to understand the mechanisms of genome folding, in this commentary we discuss its potential applicability to aspects of TE biology. Based on recent works on the relationship between genome organisation and TE integration, we argue that existing polymer models may be extended to create a predictive framework for the study of TE integration patterns. We suggest that these models may offer orthogonal and generic insights into the integration profiles (or "topography") of TEs across organisms. In addition, we provide simple polymer physics arguments and preliminary molecular dynamics simulations of TEs inserting into heterogeneously flexible polymers. By considering this simple model, we show how polymer folding and local flexibility may generically affect TE integration patterns. The preliminary discussion reported in this commentary is aimed to lay the foundations for a large-scale analysis of TE integration dynamics and topography as a function of the three-dimensional host genome
Chromatin extrusion explains key features of loop and domain formation in wild-type and engineered genomes
We recently used in situ Hi-C to create kilobase-resolution 3D maps of mammalian genomes. Here, we combine these maps with new Hi-C, microscopy, and genome-editing experiments to study the physical structure of chromatin fibers, domains, and loops. We find that the observed contact domains are inconsistent with the equilibrium state for an ordinary condensed polymer. Combining Hi-C data and novel mathematical theorems, we show that contact domains are also not consistent with a fractal globule. Instead, we use physical simulations to study two models of genome folding. In one, intermonomer attraction during polymer condensation leads to formation of an anisotropic “tension globule.” In the other, CCCTC-binding factor (CTCF) and cohesin act together to extrude unknotted loops during interphase. Both models are consistent with the observed contact domains and with the observation that contact domains tend to form inside loops. However, the extrusion model explains a far wider array of observations, such as why loops tend not to overlap and why the CTCF-binding motifs at pairs of loop anchors lie in the convergent orientation. Finally, we perform 13 genome-editing experiments examining the effect of altering CTCF-binding sites on chromatin folding. The convergent rule correctly predicts the affected loops in every case. Moreover, the extrusion model accurately predicts in silico the 3D maps resulting from each experiment using only the location of CTCF-binding sites in the WT. Thus, we show that it is possible to disrupt, restore, and move loops and domains using targeted mutations as small as a single base pair.National Science Foundation (U.S.) (Grant PHY-1427654)National Institutes of Health (U.S.) (New Innovator Award 1DP2OD008540-01)Cancer Prevention and Research Institute of Texas (Scholar Award R1304)Baylor College of Medicine (McNair Medical Institute Scholar Award)Presidential Early Career Award for Scientists and Engineer
Effectiveness of surface coatings containing silver ions in bacterial decontamination in a recovery unit
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