7 research outputs found
Genome-wide analysis of the H3K27me3 epigenome and transcriptome in brassica rapa
Background
Genome-wide maps of histone modifications have been obtained for several plant species. However, most studies focus on model systems and do not enforce FAIR data management principles. Here we study the H3K27me3 epigenome and associated transcriptome of Brassica rapa, an important vegetable cultivated worldwide.
Findings
We performed H3K27me3 chromatin immunoprecipitation followed by high-throughput sequencing and transcriptomic analysis by 3′-end RNA sequencing from B. rapa leaves and inflorescences. To analyze these data we developed a Reproducible Epigenomic Analysis pipeline using Galaxy and Jupyter, packaged into Docker images to facilitate transparency and reuse. We found that H3K27me3 covers roughly one-third of all B. rapa protein-coding genes and its presence correlates with low transcript levels. The comparative analysis between leaves and inflorescences suggested that the expression of various floral regulatory genes during development depends on H3K27me3. To demonstrate the importance of H3K27me3 for B. rapa development, we characterized a mutant line deficient in the H3K27 methyltransferase activity. We found that braA.clf mutant plants presented pleiotropic alterations, e.g., curly leaves due to increased expression and reduced H3K27me3 levels at AGAMOUS-like loci.
Conclusions
We characterized the epigenetic mark H3K27me3 at genome-wide levels and provide genetic evidence for its relevance in B. rapa development. Our work reveals the epigenomic landscape of H3K27me3 in B. rapa and provides novel genomics datasets and bioinformatics analytical resources. We anticipate that this work will lead the way to further epigenomic studies in the complex genome of Brassica crops
Genome-wide analysis of the H3K27me3 epigenome and transcriptome in brassica rapa
Background
Genome-wide maps of histone modifications have been obtained for several plant species. However, most studies focus on model systems and do not enforce FAIR data management principles. Here we study the H3K27me3 epigenome and associated transcriptome of Brassica rapa, an important vegetable cultivated worldwide.
Findings
We performed H3K27me3 chromatin immunoprecipitation followed by high-throughput sequencing and transcriptomic analysis by 3′-end RNA sequencing from B. rapa leaves and inflorescences. To analyze these data we developed a Reproducible Epigenomic Analysis pipeline using Galaxy and Jupyter, packaged into Docker images to facilitate transparency and reuse. We found that H3K27me3 covers roughly one-third of all B. rapa protein-coding genes and its presence correlates with low transcript levels. The comparative analysis between leaves and inflorescences suggested that the expression of various floral regulatory genes during development depends on H3K27me3. To demonstrate the importance of H3K27me3 for B. rapa development, we characterized a mutant line deficient in the H3K27 methyltransferase activity. We found that braA.clf mutant plants presented pleiotropic alterations, e.g., curly leaves due to increased expression and reduced H3K27me3 levels at AGAMOUS-like loci.
Conclusions
We characterized the epigenetic mark H3K27me3 at genome-wide levels and provide genetic evidence for its relevance in B. rapa development. Our work reveals the epigenomic landscape of H3K27me3 in B. rapa and provides novel genomics datasets and bioinformatics analytical resources. We anticipate that this work will lead the way to further epigenomic studies in the complex genome of Brassica crops
Midbrain microglia mediate a specific immunosuppressive response under inflammatory conditions
Background: Inflammation is a critical process for the progression of neuronal death in neurodegenerative
disorders. Microglia play a central role in neuroinflammation and may affect neuron vulnerability. Next generation
sequencing has shown the molecular heterogeneity of microglial cells; however, the variability in their response to
pathological inputs remains unknown.
Methods: To determine the effect of an inflammatory stimulus on microglial cells, lipopolysaccharide (LPS) was
administered peripherally to mice and the inflammatory status of the cortex, hippocampus, midbrain, and striatum
was assessed. Microglial activation and interaction with the immune system were analyzed in single cell
suspensions obtained from the different brain regions by fluorescence-activated cell sorting, next generation RNA
sequencing, real-time PCR, and immunohistochemical techniques. Antigen-presenting properties of microglia were
evaluated by the ability of isolated cells to induce a clonal expansion of CD4+ T cells purified from OT-II transgenic
mice.
Results: Under steady-state conditions, the midbrain presented a high immune-alert state characterized by the
presence of two unique microglial subpopulations, one expressing the major histocompatibility complex class II
(MHC-II) and acting as antigen-presenting cells and another expressing the toll-like receptor 4 (TLR4), and by the
presence of a higher proportion of infiltrating CD4+ T cells. This state was not detected in the cortex, hippocampus,
or striatum. Systemic LPS administration induced a general increase in classic pro-inflammatory cytokines, in coinhibitory programmed death ligand 1 (PD-L1), and in cytotoxic T lymphocyte antigen 4 (CTLA-4) receptors, as well
as a decrease in infiltrating effector T cells in all brain regions. Interestingly, a specific immune-suppressive response
was observed in the midbrain which was characterized by the downregulation of MHC-II microglial expression, the
upregulation of the anti-inflammatory cytokines IL10 and TGFβ, and the increase in infiltrating regulatory T cells.
Conclusions: These data show that the midbrain presents a high immune-alert state under steady-state conditions
that elicits a specific immune-suppressive response when exposed to an inflammatory stimulus. This specific
inflammatory tone and response may have an impact in neuronal viabilit
Midbrain microglia mediate a specific immunosuppressive response under inflammatory conditions
Background: Inflammation is a critical process for the progression of neuronal death in neurodegenerative
disorders. Microglia play a central role in neuroinflammation and may affect neuron vulnerability. Next generation
sequencing has shown the molecular heterogeneity of microglial cells; however, the variability in their response to
pathological inputs remains unknown.
Methods: To determine the effect of an inflammatory stimulus on microglial cells, lipopolysaccharide (LPS) was
administered peripherally to mice and the inflammatory status of the cortex, hippocampus, midbrain, and striatum
was assessed. Microglial activation and interaction with the immune system were analyzed in single cell
suspensions obtained from the different brain regions by fluorescence-activated cell sorting, next generation RNA
sequencing, real-time PCR, and immunohistochemical techniques. Antigen-presenting properties of microglia were
evaluated by the ability of isolated cells to induce a clonal expansion of CD4+ T cells purified from OT-II transgenic
mice.
Results: Under steady-state conditions, the midbrain presented a high immune-alert state characterized by the
presence of two unique microglial subpopulations, one expressing the major histocompatibility complex class II
(MHC-II) and acting as antigen-presenting cells and another expressing the toll-like receptor 4 (TLR4), and by the
presence of a higher proportion of infiltrating CD4+ T cells. This state was not detected in the cortex, hippocampus,
or striatum. Systemic LPS administration induced a general increase in classic pro-inflammatory cytokines, in coinhibitory programmed death ligand 1 (PD-L1), and in cytotoxic T lymphocyte antigen 4 (CTLA-4) receptors, as well
as a decrease in infiltrating effector T cells in all brain regions. Interestingly, a specific immune-suppressive response
was observed in the midbrain which was characterized by the downregulation of MHC-II microglial expression, the
upregulation of the anti-inflammatory cytokines IL10 and TGFβ, and the increase in infiltrating regulatory T cells.
Conclusions: These data show that the midbrain presents a high immune-alert state under steady-state conditions
that elicits a specific immune-suppressive response when exposed to an inflammatory stimulus. This specific
inflammatory tone and response may have an impact in neuronal viabilit
Microglia and astrocyte activation is region-dependent in the alfa-synuclein mouse model of Parkinson's disease
Inflammation is a common feature in neurodegenerative diseases that contributes to neuronal loss. Previously, we demonstrated that the basal inflammatory tone differed between brain regions and, consequently, the reaction generated to a pro-inflammatory stimulus was different. In this study, we assessed the innate immune reaction in the midbrain and in the striatum using an experimental model of Parkinson's disease. An adeno-associated virus serotype 9 expressing the α-synuclein and mCherry genes or the mCherry gene was administered into the substantia nigra. Myeloid cells (CD11b+ ) and astrocytes (ACSA2+ ) were purified from the midbrain and striatum for bulk RNA sequencing. In the parkinsonian midbrain, CD11b+ cells presented a unique anti-inflammatory transcriptomic profile that differed from degenerative microglia signatures described in experimental models for other neurodegenerative conditions. By contrast, striatal CD11b+ cells showed a pro-inflammatory state and were similar to disease-associated microglia. In the midbrain, a prominent increase of infiltrated monocytes/macrophages was observed and, together with microglia, participated actively in the phagocytosis of dopaminergic neuronal bodies. Although striatal microglia presented a phagocytic transcriptomic profile, morphology and cell density was preserved and no active phagocytosis was detected. Interestingly, astrocytes presented a pro-inflammatory fingerprint in the midbrain and a low number of differentially displayed transcripts in the striatum. During α-synuclein-dependent degeneration, microglia and astrocytes experience context-dependent activation states with a different contribution to the inflammatory reaction. Our results point towards the relevance of selecting appropriate cell targets to design neuroprotective strategies aimed to modulate the innate immune system during the active phase of dopaminergic degeneration
Microglia and astrocyte activation is region-dependent in the alfa-synuclein mouse model of Parkinson's disease
Inflammation is a common feature in neurodegenerative diseases that contributes to neuronal loss. Previously, we demonstrated that the basal inflammatory tone differed between brain regions and, consequently, the reaction generated to a pro-inflammatory stimulus was different. In this study, we assessed the innate immune reaction in the midbrain and in the striatum using an experimental model of Parkinson's disease. An adeno-associated virus serotype 9 expressing the α-synuclein and mCherry genes or the mCherry gene was administered into the substantia nigra. Myeloid cells (CD11b+ ) and astrocytes (ACSA2+ ) were purified from the midbrain and striatum for bulk RNA sequencing. In the parkinsonian midbrain, CD11b+ cells presented a unique anti-inflammatory transcriptomic profile that differed from degenerative microglia signatures described in experimental models for other neurodegenerative conditions. By contrast, striatal CD11b+ cells showed a pro-inflammatory state and were similar to disease-associated microglia. In the midbrain, a prominent increase of infiltrated monocytes/macrophages was observed and, together with microglia, participated actively in the phagocytosis of dopaminergic neuronal bodies. Although striatal microglia presented a phagocytic transcriptomic profile, morphology and cell density was preserved and no active phagocytosis was detected. Interestingly, astrocytes presented a pro-inflammatory fingerprint in the midbrain and a low number of differentially displayed transcripts in the striatum. During α-synuclein-dependent degeneration, microglia and astrocytes experience context-dependent activation states with a different contribution to the inflammatory reaction. Our results point towards the relevance of selecting appropriate cell targets to design neuroprotective strategies aimed to modulate the innate immune system during the active phase of dopaminergic degeneration
Uncovering perturbations in human hematopoiesis associated with healthy aging and myeloid malignancies at single-cell resolution
Early hematopoiesis is a continuous process in which hematopoietic stem and progenitor cells (HSPCs) gradually differentiate toward specific lineages. Aging and myeloid malignant transformation are characterized by changes in the composition and regulation of HSPCs. In this study, we used single-cell RNA sequencing (scRNA-seq) to characterize an enriched population of human HSPCs obtained from young and elderly healthy individuals.
Based on their transcriptional profile, we identified changes in the proportions of progenitor compartments during aging, and differences in their functionality, as evidenced by gene set enrichment analysis. Trajectory inference revealed that altered gene expression dynamics accompanied cell differentiation, which could explain aging-associated changes in hematopoiesis. Next, we focused on key regulators of transcription by constructing gene regulatory networks (GRNs) and detected regulons that were specifically active in elderly individuals. Using previous findings in healthy cells as a reference, we analyzed scRNA-seq data obtained from patients with myelodysplastic syndrome (MDS) and detected specific alterations of the expression dynamics of genes involved in erythroid differentiation in all patients with MDS such as TRIB2. In addition, the comparison between transcriptional programs and GRNs regulating normal HSPCs and MDS HSPCs allowed identification of regulons that were specifically active in MDS cases such as SMAD1, HOXA6, POU2F2, and RUNX1 suggesting a role of these transcription factors (TFs) in the pathogenesis of the disease.
In summary, we demonstrate that the combination of single-cell technologies with computational analysis tools enable the study of a variety of cellular mechanisms involved in complex biological systems such as early hematopoiesis and can be used to dissect perturbed differentiation trajectories associated with perturbations such as aging and malignant transformation. Furthermore, the identification of abnormal regulatory mechanisms associated with myeloid malignancies could be exploited for personalized therapeutic approaches in individual patient