Protein-Resistant Surfaces through Mild Dopamine Surface Functionalization

The synthesis and evaluation of new dopamine-based catechol anchors coupled to poly(ethylene glycol) (PEG) for surface modification of TiO2 are reported. Dopamine is modified by dimethylamine-methylene or trimethylammonium- methylene groups, and the preparation of mPEG-Glu didopamine polymer 11 is presented. All these PEG polymers allow stable adlayers on TiO2 to be generated through mild dip-and-rinse procedures, as evaluated both by variable angle spectroscopic ellipsometry and X-ray photoelectron spectroscopy. The resulting surfaces substantially reduced protein adsorption upon exposure to full human serum

Catechol functionalized polymers and method for preparing them

Described are compounds that are capable of forming adlayers, said compounds comprising at least one headgroup A, an optional linker B, a polymer C, a terminal group D and an optionally present crosslinking group R, wherein the headgroup A is a compound of the following formula wherein X is independently from each other selected from an acidic group, such as OH, SH, phosphate, phosphornate and N+R2H, Y is a group derived from a convenient coupling group, in particular NL3, or N+(L3)2, Z1, Z3, and Z4 are C or N+ Z2, Z5 and Z6 are C-L1 or N+-L, wherein L1 is H or an electron withdrawing group, L is C1-C6 alkyl, in particular C1-C4 alkyl L2 and L3 are independently selected from H or C1-C6 alkyl, preferably C1-C4 alkyl, or L2 may form together with L1 or L of Z6 a heterocycle, in particular a positively charged heterocycle, n is 0, or 1, or 2, or 3, m is 0 or 1 with the proviso that at most one of Z1 to Z6 is N+ and that in case that Z1 is N+, L2 may additionally be an ester, an amide, or a heterocycle

Enhanced bone apposition around biofunctionalized sandblasted and acid-etched titanium implant surfaces. A histomorphometric study in miniature pigs

Microrough titanium (Ti) surfaces of dental implants have demonstrated more rapid and greater bone apposition when compared with machined Ti surfaces. However, further enhancement of osteoblastic activity and bone apposition by bio-functionalizing the implant surface with a monomolecular adsorbed layer of a co-polymer - i.e., poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) and its derivatives (PLL-g-PEG/PEG-peptide) - has never been investigated. The aim of the present study was to examine early bone apposition to a modified sandblasted and acid-etched (SLA) surface coated with an Arg-Gly-Asp (RGD)-peptide-modified polymer (PLL-g-PEG/PEG-RGD) in the maxillae of miniature pigs, and to compare it with the standard SLA surface. Test and control implants had the same microrough topography (SLA), but differed in their surface chemistry (polymer coatings). The following surfaces were examined histomorphometrically: (i) control - SLA without coating; (ii) (PLL-g-PEG); (iii) (PLL-g-PEG/PEG-RDG) (RDG, Arg-Asp-Gly); and (iv) (PLL-g-PEG/PEG-RGD). At 2 weeks, RGD-coated implants demonstrated significantly higher percentages of bone-to-implant contact as compared with controls (61.68% vs. 43.62%; P < 0.001). It can be concluded that the (PLL-g-PEG/PEG-RGD) coatings may promote enhanced bone apposition during the early stages of bone regeneration