Identity ranges (%) of the 3 ORFs both at amino acid (bold) and nucleotide level between species in the PARV4-like viruses^a.

a<p>Eh-BtPV-1 (<i>Eidolon helvum Bat Parvovirus</i> 1), PARV4-g1/3 (<i>Human Parvovirus 4</i> genotypes 1, NC_007018, 2, DQ873391, 3, EU874248), ChimpPTV (<i>Chimpanzee PARV4</i>, HQ113143), PHoV (<i>Porcine Hokovirus</i>, EU200673), BHoV (<i>Bovine Hokovirus</i>, EU200670).</p

Three types of Aj-BtPV-1.

<p>The phylogenetic tree was based on a 740 nt fragment (nucleotides 3820–4561) of Aj-BtPV identified in <i>Artibeus jamaicensis</i> and <i>Artibeus lituratus</i> (#174) bats from Panama, showing the 3 different viral types (panel A). Identity values (in percentage) within and between the 3 types are shown in panel B.</p

Primers used in this study for screening, sequencing and quantifying Eh-BtPV-1 and Aj-BtPV-1.

<p>Primers used in this study for screening, sequencing and quantifying Eh-BtPV-1 and Aj-BtPV-1.</p

Detection of Eh-BtPV-1 in different body compartments of 7 <i>Eidolon helvum</i> bats.^a

a<p>Viral concentrations are indicated as copies/ml in serum and copies/g of tissue obtained from different organs; NEG = negative; NA = not available. The boldface viral concentrations indicate organs with a concentration at least 10 fold higher than serum.</p

Genome organization of Eh-BtPV-1 and of representative members of the PARV4-like genus.

<p>Indicated are the main ORFs (gray, coding frame indicated in parenthesis) and the PLA₂ motif position (purple). On the top the identity within the alignment is shown. Accession numbers: PHoV, EU200673; BHoV, EU200670; ChimpPTV, HQ113143; PARV4, AY622943. Figure was made with Geneious v5.1 software <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029140#pone.0029140-Drummond1" target="_blank">[52]</a>.</p

Recombinant HA1-proteins included in the protein microarray.

<p>Proteins were obtained from Immune Technology Corp. (NY, USA) or Sino Biological Inc. (Beijing, China).</p><p>^ahighly pathogenic</p><p>^blow pathogenic</p><p>Recombinant HA1-proteins included in the protein microarray.</p

Titers of individual Ghanaian bats plotted against all recombinant proteins included on the microarray.

<p>Horizontal bars represent geometric mean titers per antigen including a 95% confidence interval. Sera below fluorescence values of 31.268 (half of the fluorescence spectrum) were regarded as negative and were assigned a titer of 20 (half of the starting dilution of 1:40). Sera above 40 (dashed line) were regarded as positive. Arrows indicate antigens grouping within the same phylogenetic cluster.</p