SDF-1β increases the number of surviving BMSCs following exposure to H₂O₂.

<p>Cell number of trypan blue-stained A) Tet-Off-SDF-1β BMSCs and B) Tet-Off-EV control BMSCs after vehicle control or H₂O₂ treatment. SDF-1β significantly increased the number of surviving cells (trypan blue negative) and decreased the number of dying cells (trypan blue positive) in response to H₂O₂ treatment compared to Dox-suppressed and Tet-Off-EV controls (6 h, ±100 ng/ml Dox, ±1.0 mM H₂O₂, ***p<0.0001, −Dox; H₂O₂ vs. +Dox; H₂O₂, n = 3, 3 independent experiments).</p

SDF-1β preserves BMSC nuclear morphology following exposure to H₂O₂.

<p>Representative fluorescence micrographs of Hoechst 33342-stained A) Tet-Off-SDF-1β BMSCs and B) Tet-Off-EV control BMSCs after vehicle control or H₂O₂ treatment. Overexpression of SDF-1β in Tet-Off-SDF-1β BMSCs allows for a greater number of surviving cells and cells with preserved nuclear morphology after H₂O₂ treatment compared to Dox-suppressed and Tet-Off-EV controls (6 h, ±100 ng/ml Dox, ±1.0 mM H₂O₂, 20×, 40×, bar 100 µm, n = 3, 3 independent experiments).</p

SDF-1β does not affect BMSC proliferation.

<p>Colorimetric quantification of DMSO-solubilized MTT formazan at 540 nm showed no differences in proliferation of Tet-Off-SDF-1β compared to Dox-suppressed and Tet-Off-EV controls (1,3, and 7 d, ±100 ng/ml Dox, n = 6, 3 independent experiments).</p

SDF-1β preserves BMSC morphology following exposure to H₂O₂.

<p>Representative phase contrast micrographs of A) Tet-Off-SDF-1β BMSCs and B) Tet-Off-EV control BMSCs after vehicle control or H₂O₂ treatment. Overexpression of SDF-1β in Tet-Off-SDF-1β BMSCs allows for a greater number of cells with preserved morphology after H₂O₂ treatment relative to Dox-suppressed and Tet-Off-EV controls (6 h, ±100 ng/ml Dox, ±1.0 mM H₂O₂, 20×, 40×, bar 100 µm, n = 3, 3 independent experiments).</p