Maximum Likelihood tree generated from amino acid sequences of vertebrate ferritins.

<p>The various ferritin sequences are clustered according to chain types, with heavy(H)-chains forming an orthologous group to the middle (M)/light(L)-chains. Ferritin sequences from <i>S. salar</i> (H1, H2, M1, M2, M3) characterised in this study are highlighted in green, while previously reported sequences <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103729#pone.0103729-Andersen1" target="_blank">[13]</a> are indicated with (*). Values at nodes indicate the Maximum-Likelihood bootstrap percentages (1000 replications). The scale bar represents the estimated number of substitutions per site.</p

Fold changes in the expression of the ferritin H- and M-chain isoforms (H, M1, M2, M3) in <i>S. salar</i> muscle cell culture stimulated with IL-1β after A. 6 hour, B. 24 hours and C. 48 hours.

<p>Bars represent standard errors mean (± SEM, n = 4) and asterisks indicate significant differences (p<0.05, <i>t</i>-test).</p

List of PCR primers used in this study.

<p>List of PCR primers used in this study.</p

Tissue distribution of the ferritin H- and M-chain isoforms (H, M1, M2, M3) in <i>S. salar</i>.

<p>The relative expression of H-chain was measured as the total expression of the H1 and H2 isoforms. The relative expression of each isoform was normalised to the averaged expression of <i>ef1α</i> and <i>βact</i>. Bars represent standard errors mean (± SEM, n = 4).</p

Comparison of the gene organisation of H1, H2, M1, M2 and M3 from <i>S. salar</i> with ferritin sequences from other vertebrates.

<p>Exons and introns are represented respectively by the blue boxes and black lines. Numbers below and above the boxes respectively indicate exon sizes and intron sizes. The length of exons and introns is drawn to scale except for intron sizes exceeding 1500<b>//</b>.</p

Nucleotide sequences of the Atlantic salmon ferritin cDNAs A) Nucleotide sequences of the Atlantic salmon ferritin cDNA clones H1, H2, M1, M2 and M3.

<p>Identical nucleotide residues are indicated by periods, while substituted residues are shown. The start and termination codons are underlined. B) Amino acid sequences based on the predicted ORFs of H1, H2, M1, M2 and M3. Identical amino acid residues are indicated by periods and substituted residues are shown. The conserved ferroxidase centres and nucleation sites are respectively indicated by <b>*</b> and boxes.</p

Transcriptional modulation of trout <i>gpx1</i>, <i>trxr3</i>, and <i>selp</i> isoforms in blood cells (A) and muscle (B) from rainbow trout fed a control diet and three diets enriched with different concentrations (0.5, 4 and 8 g Kg^-1) of Sel-Plex for 2, 6 and 10 weeks.

<p>The expression of gene transcripts was quantified by <i>q</i>PCR and normalized against the geometric mean of three housekeeping genes (<i>ef-1α</i>, <i>drpII</i>, <i>hprt</i>) from the same sample, and then used for statistical analysis. A fold change, calculated as the average expression level of stimulated samples divided by that of the controls, is presented. The results represent the mean + SEM from six fish. The letters above the columns indicate values statistically significant from the controls (<i>p<</i>0.05), with different letters indicating significant differences between the treatments.</p

Se concentration in the feed pellets of the four different diets used for this feeding trial.

<p>The results represent the mean and SEM of four independent measurements.</p><p>Se concentration in the feed pellets of the four different diets used for this feeding trial.</p

Growth performance of rainbow trout given various levels of organic supplemental Se for 10 weeks.

<p>The results represent the mean <b>±</b> SEM from six fish per diet at each time point. On the graph the significance of the difference in body weight between the diets groups at each time point is indicated, whilst in the table underneath the changes in body weight for each diet group across the different time points is reported. Values not statically different are indicated by “n.s.”, whereas values significantly different (<i>p<</i>0.05) are indicated by different letters.</p

Transcriptional modulation of trout <i>gpx1</i>, <i>trxr3</i>, and <i>selp</i> isoforms in liver (A) and kidney (B) from rainbow trout fed a control diet and three diets enriched with different concentrations (0.5, 4 and 8 g Kg^-1) of Sel-Plex for 2, 6 and 10 weeks.

<p>The expression of gene transcripts was quantified by <i>q</i>PCR and normalized against the geometric mean of three housekeeping genes (<i>ef-1α</i>, <i>drpII</i>, <i>hprt</i>) from the same sample, and then used for statistical analysis. A fold change, calculated as the average expression level of stimulated samples divided by that of the controls, is presented. The results represent the mean + SEM from six fish. The letters above the columns indicate values statistically significant from the controls (<i>p<</i>0.05), with different letters indicating significant differences between the treatments.</p