SP-Sephadex equilibrium chromatography of bradykinin and related peptides: Application to trypsin-treated human plasma

An analytical method is deseribed for the separation of bradykinin, Lys-bradykinin, and Met-Lys-bradykinin by equilibrium chromatography on SP-Sephadex C-25 eluted in 0.02 Tris-HCl buffer, pH 8.10, 0.12 NaCl. A second elution buffer, 0.02 Tris-HCl buffer, pH 7.70, 0.06 NaCl, serves as a second parameter for the identification of bradykinin and also separates the hormone from plasma bradykinin-potentiating peptides. Ten to one-hundred nanomoles of each peptide can be recovered in high yields, identified by elution position, and measured by bioassay with the isolated guinea pig ileum. The identification of bradykinin as the peptide released by trypsin acting on acid-denatured plasma is documented as an illustration of the method

Pancreas Burkitt primary lymphoma in pediatric age

We present an the case of an eight-year-old Portuguese boy with no history of traveling, admitted with non-specific abdominal pain. An analytic study revealed high levels of lipase and amylase. Ultrasound, abdominal computerized tomography (CT), and an abdominal magnetic resonance imaging (MRI), were performed. The imaging findings are suggestive of a pancreatic tumor which is an extremely rare entity in children. A biopsy was performed by opened surgery and identified a Burkitt lymphoma.info:eu-repo/semantics/publishedVersio

Enzymatic production of fructose fatty acid ester using lipases from C. antarctica and porcine pancreatic

The aim of this work was to produce fructose fatty acid ester by enzymatic esterification of a fatty acid (oleic acid or linoleic acid) with fructose, using lipases (CALB) from Candida antarctica type B and porcine pancreas. The esterification reaction was conducted at 150 rpm and 40 °C during 72 hours. Equimolar (0.5 mmol) amounts of fructose and fatty acid were mixed with 0.6 ml of ethanol and sodium sulfate anhydrous (0.1 g) was added for the adsorption of the water generated during the reaction. In all experiments, 22.5 mg of lipase were used. A control experiment was performed using the same conditions except for the addition of lipase. Samples were analyzed by thin layer chromatography (TLC), using silica gel plates. The plates were placed in an iodine chamber to develop fatty acid and sugar ester spots. According to the literature an Rf of 0.5 is expected for the fructose fatty acid ester using chloroform/ hexane (1:1, v/ v) as eluting solvent. Four different reaction schemes were studied in this work namely, sample 1 (oleic acid, fructose, C. antarctica lipase, sodium sulfate and ethanol), sample 2 (oleic acid, fructose, porcine pancreatic lipase, sodium sulfate and ethanol), sample 3 (linoleic acid, fructose, C. antarctica lipase, sodium sulfate and ethanol) and sample 4 (linoleic acid, fructose, porcine pancreatic lipase, sodium sulfate and ethanol). From the TLC assays, the formation of fructose ester was observed for samples 1, 2 and 3. Additionally, the yield of esterification was determined by calculating the amount of residual fatty acid in the reaction mixture, which was determined using a volumetric method, as described elsewhere. Esterification yields of 74.3, 41.4, 63.5 and 11.2 % (v/ v) were determined for samples 1 to 4, respectively. Therefore, it was possible to conclude that the lipase obtained from C. antarctica had a better performance than the one obtained from porcine pancreas. Furthermore, the reaction schemes that used oleic acid conducted to higher yields of fructose ester production. These results point out that the enzymatic production of fructose esters is worthwhile and suggest the need for further research

Seleção e avaliação de matrizes de guaranazeiro para produção de sementes (Paulinia cupana var. Sorbilis Mart. Duke).

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Exotic looped trajectories via quantum marking

We provide an analytical and theoretical study of exotic looped trajectories (ELTs) in a double-slit interferometer with quantum marking. We use an excited Rydberg-like atom and which-way detectors such as superconducting cavities, just as in the Scully-Englert-Walther interferometer. We indicate appropriate conditions on the atomic beam or superconducting cavities so that we determine an interference pattern and fringe visibility exclusive from the ELTs. We quantitatively describe our results for Rubidium atoms and propose this framework as an alternative scheme to the double-slit experiment modified to interfere only these exotic trajectories.Comment: 10 pages, 5 figure

Incidence and Health Related Quality of Life of Opioid-Induced Constipation in Chronic Noncancer Pain Patients: A Prospective Multicentre Cohort Study

High rates of opioid use for chronic noncancer pain (CNCP) have been reported worldwide, despite its association with adverse events, inappropriate use, and limited analgesic effect. Opioid-induced constipation (OIC) is the most prevalent and disabling adverse effect associated with opioid therapy. Our aim was to assess the incidence, health related quality of life (HRQOL), and disability in OIC patients.This work was supported by intramural funds from the Chair on Pain Medicine of the Faculty of Medicine, University of Porto, and by projects “Porto Neurosciences and Neurologic Disease Research Initiative at i3S” (NORTE-01-0145-FEDER-000008) and “NanoSTIMA” (NORTE-01-0145-FEDER-000016), which are financed by the North Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, and through the European Regional Development Fund (ERDF).info:eu-repo/semantics/publishedVersio

Coupling and induced depinning of magnetic domain walls in adjacent spin valve nanotracks

The magnetostatic interaction between magnetic domain walls (DWs) in adjacent nanotracks has been shown to produce strong inter-DW coupling and mutual pinning. In this paper, we have used electrical measurements of adjacent spin-valve nanotracks to follow the positions of interacting DWs. We show that the magnetostatic interaction between DWs causes not only mutual pinning, as observed till now, but that a travelling DW can also induce the depinning of DWs in near-by tracks. These effects may have great implications for some proposed high density magnetic devices (e.g. racetrack memory, DW logic circuits, or DW-based MRAM).Comment: The following article has been accepted by the Journal of Applied Physic