Can histopathological evidence of ankylosis of the tooth be helpful in the prevention of a medicolegal case in case of iatrogenic fracture of the mandible during dental extraction where preoperative radiographs have not been taken?

A fracture of the mandible is a rare complication that can occur during a dental extraction being carried out under local/general anesthesia. It is always advisable to take a pre-operative radiograph of the tooth to be considered for extraction to study the root configuration and make a surgical plan for the case. Most of the dental extractions carried out without taking a preoperative radiograph are completed uneventfully. However, in rare cases, complications can arise owing to local anatomical variations and lead to medicolegal issues and litigation. This article discusses the management of a case of iatrogenic fracture of the mandible during dental extraction, wherein a pre-operative radiograph was not taken; however, following extraction of the tooth and management of the fracture mandible, the histopathology report of the extracted tooth was suggestive of ankylosis of the tooth

Challenges with the use of Xpert HPV as a screening tool for oral HPV among people living with HIV (PLHIV): experiences from Pune, India

Abstract Background People living with HIV (PLHIV) are at higher risk for human papillomavirus (HPV)-related oropharyngeal cancers compared to the general population. Xpert HPV test is a polymerase chain reaction (PCR) assay capable of rapid HPV detection. Performing the assay requires minimal intervention by laboratory personnel. Its use could improve oropharyngeal cancer screening among PLHIV living in low-and middle-income countries (LMICs) with limited diagnostic capacities. However, Xpert HPV performance for oral samples has not been evaluated. Here, we describe our experience with Xpert HPV and compare its results with traditional PCR, for oral samples. Methods Oral samples from 429 PLHIV receiving care at a tertiary care hospital affiliated antiretroviral therapy center in Pune, India were used. Samples were collected either after a 30s oral rinse and gargle (n = 335) or in combination with cytobrush scraping of the oral mucosa (n = 91). Unsuccessful tests were those that generated an invalid or error result on Xpert HPV. Successful tests were those that generated a positive or negative result. Kappa statistic was used to compare concordance between Xpert HPV and traditional real-time PCR results. Results There were 29.8% (n = 127) unsuccessful tests, of which 78.7% (n = 100) were invalid and 21.3% (n = 27) were error results. Adding cytobrush scraping to oral rinse as a collection procedure did not significantly reduce the proportion of unsuccessful tests (p = 0.9). For successful tests, HPV positivity on Xpert was 0.3% (n = 1/299). Kappa statistic was 0.11, indicating poor agreement between Xpert HPV and traditional PCR results. Conclusions Presently, Xpert HPV appears to have limited use for oral HPV detection among PLHIV using oral samples. More research to improve the diagnostic capabilities of Xpert HPV for oral samples among PLHIV is needed

Additional file 1 of Challenges with the use of Xpert HPV as a screening tool for oral HPV among people living with HIV (PLHIV): experiences from Pune, India

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