Criteria for tumor resectability.

<p>Intraoperative decision and percentages of surgical (non-) resectability in PDAC.</p

Questionnaire consisting of twelve question blocks with a total of 34 parameters.

<p>Questionnaire consisting of twelve question blocks with a total of 34 parameters.</p

Factorial analysis by anti-image-matrix (A) and Varimax-matrix rotation (B).

<p>Sufficient suitability of microRNA expression and etiological parameters with coefficients >0.5 (^a suitability criteria). Rotated matrix of components shows a high factorial load by combining microRNA expression and HCC. Factor annotations of initial variables are in bold.</p

Diagnostic performance of microRNA-141 and microRNA-200a.

<p>Receiver operating characteristic (ROC) curve of microRNA-141 (A) and microRNA-200a (B) in three groups (Healthy <i>vs</i>. HCC; Healthy <i>vs</i>. Cirrhosis; HCC <i>vs</i>. Cirrhosis). Area under the curve (AUC) values are presented by the estimate with 95% confidence interval.</p

The ΔCt expression level of the five circulating microRNA-200 family members.

<p>ΔCt levels are inversely proportional to the amount of target microRNA in the sample. Asterisks indicate to a significant difference of <i>p</i>< 0.05, respectively.</p

MicroRNA profiling.

<p>Procedure and results of miR expression profiling by GeneChip microarray and qRT-PCR validation in parental and gemcitabine resistant PANC-1 and MIA-PaCa-2 cell clones.</p

Establishment of chemoresistant PDAC cell clones.

<p>Generation of chemoresistant PDAC cell clones by repeated pulsatile treatment over 3 days with constant sublethal concentrations of 0.4μM (A) or 0.06μM (D) gemcitabine followed by recovery-periods and quantification of cell viability by MTT assay as well as apoptosis assay in parental vs. chemoresistant PANC-1 (A, B, C) and MIA-PaCa-2 (D, E, F) cell clones.</p

Interaction of mutant TP53 and microRNA signaling pathways in chemoresistant pancreatic cancer.

<p>Target analysis was performed by Ingenuity Pathway Analysis and DIANA-mirPath.</p

Protein target expression.

<p>Protein expression of MRP-1, mutant p53 (mt p53 R273H and mt p53 R248W), CDK1, Bcl-2, and actin (loading control) in parental (PANC-1, MIA-PaCa-2) and gemcitabine resistant PDAC cell clones (PANC-1-GR, MIA-PaCa-2-GR) by Western blot.</p

In-silico target analysis.

<p>Overview of selected and highly predicted gene targets of dysregulated miRs in chemoresistant PDAC cell clones. (N.A., not available)</p